In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_6 ( 2015-06-01), p. 1889-1894
Abstract:
A Gram-staining-negative, strictly heterotrophic and aerobic bacterium, strain TS-T44 T , was isolated from a saline lake, Tuosu Lake in Qaidam basin, Qinghai province, China. Its taxonomic position was investigated using a polyphasic approach. Cells of strain TS-T44 T were non-endospore-forming, non-motile rods, 0.8–1.2 μm wide and 1.2–3.0 μm long. Catalase- and oxidase-positive. Growth occurred in the presence of up to 8 % (w/v) NaCl (optimum, 3.0 %) and at 15–35 °C (optimum, 25 °C) and pH 7.0–10.0 (optimum, pH 7.5–8.5). C 18 : 1 ω7 c was the predominant fatty acid. The major respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and an unknown lipid. The DNA G+C content was 65.5 mol% [determined from the melting temperature ( T m)]. Phylogenetic trees based on 16S rRNA gene sequences showed that strain TS-T44 T was associated with the genus Marivita and showed highest sequence similarity to Marivita cryptomonadis CL-SK44 T (97.7 %), Marivita litorea CL-JM1 T (97.5 %) and Marivita geojedonensis DPG-138 T (97.3 %), and 〈 97 % to other species. DNA–DNA relatedness of strain TS-T44 T to M. cryptomonadis JCM 15447 T , M. litorea JCM 15446 T and M. geojedonensis KCTC 23882 T was 23 ± 3 %, 33 ± 4 % and 35 ± 2 %, respectively. Based on the data presented, it is concluded that strain TS-T44 T represents a novel species of the genus Marivita , for which the name Marivita lacus sp. nov. is proposed. The type strain is TS-T44 T ( = CGMCC 1.12478 T = JCM 19516 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.000195
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
Permalink