In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 60, No. 9 ( 2010-09-01), p. 2231-2235
Abstract:
An obligately anaerobic, Gram-positive, spore-forming bacterial strain, designated SL206 T , was isolated from pear orchard soils. Strain SL206 T cells were straight or slightly curved rods, with motility by peritrichate flagella. Cell walls contained meso -diaminopimelic acid; wall sugars were glucose, rhamnose and mannose. The major fatty acids were C 16 : 0 , C 18 : 1 ω 9 c and summed feature 10 (containing C 18 : 1 ω 11 c /9 t /6 t ). API 20A reactions were negative for oxidase, catalase and acid production from l -rhamnose, sucrose, trehalose, d -xylose, melezitose, salicin and d -sorbitol, and positive for acid production from d -glucose, sucrose, maltose, d -mannose and raffinose. Glucose was fermented to acetate, butyrate, CO 2 , H 2 and ethanol in culture. The G + C content of the genomic DNA was 31.1 mol%. Based on comparative 16S rRNA gene sequence analysis, the isolate belonged to the genus Clostridium and formed a clade with Clostridium pasteurianum . The species most closely related to strain SL206 T were C. pasteurianum (98.6 % similarity) and Clostridium acidisoli (97.8 % similarity). In DNA–DNA relatedness studies, the isolate had 59.5 % relatedness with C. pasteurianum and thus represented a unique species. On the basis of these studies, strain SL206 T (=KCTC 5449 T =JCM 14858 T ) is proposed to represent the type strain of a novel species, Clostridium arbusti sp. nov.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.013953-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2010
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
Permalink
