In:
Acta Crystallographica Section D Biological Crystallography, International Union of Crystallography (IUCr), Vol. 61, No. 6 ( 2005-06-01), p. 737-743
Abstract:
A previous study showed that the diffraction from cubic crystals of an icosahedral virus, cowpea mosaic virus (CPMV), was dramatically improved under elevated hydrostatic pressure. This use of pressure may have a significant impact on structural biology if it is found to be generally applicable. There were two types of cubic crystals assigned in either an I 23 or P 23 space group. They show the same rhombic dodecahedral morphology at atmospheric pressure. The crystals assigned to the I 23 space group diffracted X-rays to higher resolution than those with P 23 space group. The assignment of P 23 space group was owing to the presence of reflections with indices of h + k + l = (2 n + 1) (odd reflections), which are forbidden in space group I 23. Analysis of the odd reflections from the P 23 crystals at atmospheric pressure showed that they can originate from a rotational disorder in the I 23 crystals. The odd reflections were eliminated with the application of 3.5 kbar of pressure, which transformed the crystals from the apparently primitive cell to the body-centered I 23 space group with dramatic improvement in diffraction. A mechanistic model is proposed to describe the induction of order by rectifying the imperfection, which is consistent with the experimental data.
Type of Medium:
Online Resource
ISSN:
0907-4449
DOI:
10.1107/S0907444905000053
Language:
Unknown
Publisher:
International Union of Crystallography (IUCr)
Publication Date:
2005
detail.hit.zdb_id:
2968623-4
SSG:
12
SSG:
13
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