In:
Genetical Research, Hindawi Limited, Vol. 40, No. 2 ( 1982-10), p. 175-190
Abstract:
In(IL; IR)O Y348 is a pericentric inversion of linkage group I in N. crassa , with a breakpoint between fr and un-5 in the left arm and a breakpoint between ad-9 and nit-1 in the right arm. Approximate breakpoint location was found by tabulating crossovers between the rearrangement and markers in normal chromosome sequence. Inversion structure was verified by marked In O Y348 × In O Y348 crosses. Precise mapping of breakpoints was by duplication coverage. Inversions like O Y348 do not produce progeny with segmental chromosome duplications when crossed to normal sequence, but duplications were produced by crossing it to In(IL; IR)O Y323 (Barry & Leslie, 1982), another standard pericentric inversion, and to T(I → VI)NM103 (Turner, 1977), a translocation to a tip. Each of these rearrangements has a breakpoint within the inverted region of In O Y348 . Two duplications from In O Y348 × In O Y323 were converted to normal chromosome sequence by double mitotic recombination. Besides expediting mapping, the technique of intercrossing rearrangements increasingly enables us to make segmental duplications exactly tailored for studying specific included genes.
Type of Medium:
Online Resource
ISSN:
0016-6723
,
1469-5073
DOI:
10.1017/S0016672300019042
Language:
English
Publisher:
Hindawi Limited
Publication Date:
1982
detail.hit.zdb_id:
2412684-6
detail.hit.zdb_id:
1472156-9
SSG:
12
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