In:
Phycological Research, Wiley, Vol. 60, No. 4 ( 2012-10), p. 316-325
Abstract:
Red tides of C hattonella spp. have caused continuous damage to J apanese aquaculture, however, the life cycle of this organism remains incompletely understood. To further investigate this matter, we assessed genotypes at 14 microsatellite markers in three varieties of C hattonella marina , viz., C . marina var. antiqua , C . marina var. marina , and C . marina var. ovata , to establish whether C hattonella undergoes asexual diploidization or sexual reproduction. After genotyping 287 strains of C . marina , all but one of these strains was shown to be heterozygous for at least some loci, and thus, in the diploid state, suggesting that C hattonella strains undergo sexual reproduction. In addition, we performed single‐cell amplification on ‘small cells’ that are derived from vegetative cells under dark and low‐nutrient conditions. The results indicated the existence of two types of small cells. The ‘ S mall cell T ype 1’ was found to be heterozygous, genotypically equivalent to the vegetative cells, and is therefore diploid. These small cells may change to resting cells (cysts) directly. The ‘ S mall cell T ype 2’ was homozygous at all analyzed loci, suggesting that these small cells are haploid and may be derived by meiosis. As fusion between small cells has previously been observed, the ‘ S mall cell T ype 2’ may be the gamete of C hattonella . We present a construct of the full life cycle of C hattonella marina based on our own and previous results.
Type of Medium:
Online Resource
ISSN:
1322-0829
,
1440-1835
DOI:
10.1111/pre.2012.60.issue-4
DOI:
10.1111/j.1440-1835.2012.00662.x
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
2020835-2
detail.hit.zdb_id:
1312995-8
SSG:
12
Permalink