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  • Canadian Science Publishing  (4)
  • Biodiversity Research  (4)
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  • Canadian Science Publishing  (4)
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  • Biodiversity Research  (4)
  • 1
    Online Resource
    Online Resource
    Preliminary interspecific genetic maps of the 〈i〉Populus〈/i〉 genome constructed from RAPD markers
    Canadian Science Publishing ; 2001
    In:  Genome Vol. 44, No. 4 ( 2001), p. 602-609
    Details
    In: Genome, Canadian Science Publishing, Vol. 44, No. 4 ( 2001), p. 602-609
    Type of Medium: Online Resource
    ISSN: 1480-3321 , 0831-2796
    URL: Article
    DOI: 10.1139/gen-44-4-602
    Language: Unknown
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 2020635-5
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Mapping epigenetic quantitative trait loci (QTL) altering a developmental trajectory
    Canadian Science Publishing ; 2002
    In:  Genome Vol. 45, No. 1 ( 2002-02-01), p. 28-33
    Details
    In: Genome, Canadian Science Publishing, Vol. 45, No. 1 ( 2002-02-01), p. 28-33
    Abstract: Genetic variation in a quantitative trait that changes with age is important to both evolutionary biologists and breeders. A traditional analysis of the dynamics of genetic variation is based on the genetic variancecovariance matrix among different ages estimated from a quantitative genetic model. Such an analysis, however, cannot reveal the mechanistic basis of the genetic variation for a growth trait during ontogeny. Age-specific genetic variance at time t conditional on the causal genetic effect at time t 1 implies the generation of episodes of new genetic variation arising during the interval t 1 to t. In the present paper, the conditional genetic variance estimated from Zhu's (1995) conditional model was partitioned into its underlying individual quantitative trait loci (QTL) using molecular markers in an F 2 progeny of poplars (Populus trichocarpa and Populus deltoides). These QTL, defined as epigenetic QTL, govern the alterations of growth trajectory in a population. Three epigenetic QTL were detected to contribute significantly to variation in growth trajectory during the period from the establishment year to the subsequent year in the field. It is suggested that the activation and expression of epigenetic QTL are influenced by the developmental status of trees and the environment in which they are grown.Key words: epigenetic modification, development, marker, poplar, QTL.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g01-118
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2002
    detail.hit.zdb_id: 2020635-5
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Preliminary interspecific genetic maps of the Populus genome constructed from RAPD markers
    Canadian Science Publishing ; 2001
    In:  Genome Vol. 44, No. 4 ( 2001-08-01), p. 602-609
    Details
    In: Genome, Canadian Science Publishing, Vol. 44, No. 4 ( 2001-08-01), p. 602-609
    Abstract: We have constructed RAPD-based linkage maps for an interspecific cross between two species of the genus Populus (P. adenopoda and P. alba), based on a double pseudo-test-cross strategy. Of a total of 360 polymorphic fragments scored, 290 showed a test-cross configuration, corresponding to DNA polymorphisms heterozygous in one parent and null in the other. In the female parent, P. adenopoda, 82 markers were grouped in 19 different linkage groups (553 cM), whereas in the male parent P. alba, 197 markers established a much more complete framework map with an observed genome length of 2300 cM covering 87% of the total P. alba genome. The larger number of test-cross markers detected for the P. alba parent than for the P. adenopoda parent might be due to a higher level of heterozygosity in the former than in the latter. In this study, we detected only a small percentage (2%) of the intercross dominant markers heterozygous in both parents and segregating 3:1 in the progeny. The further focus in this mapping study should be on the identification of more intercross markers, to align the two parent-specific maps into a consensus map for mapping important genes causing species differentiation during long evolutionary divergences.Key words: interspecific hybrids, linkage map, poplar, pseudo-testcross mapping strategy.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g01-054
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 2020635-5
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Molecular linkage maps of the Populus genome
    Canadian Science Publishing ; 2002
    In:  Genome Vol. 45, No. 3 ( 2002-06-01), p. 541-555
    Details
    In: Genome, Canadian Science Publishing, Vol. 45, No. 3 ( 2002-06-01), p. 541-555
    Abstract: We report molecular genetic linkage maps for an interspecific hybrid population of Populus, a model system in forest-tree biology. The hybrids were produced by crosses between P. deltoides (mother) and P. euramericana (father), which is a natural hybrid of P. deltoides (grandmother) and P. nigra (grandfather). Linkage analysis from 93 of the 450 backcross progeny grown in the field for 15 years was performed using random amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs), and inter-simple sequence repeats (ISSRs). Of a total of 839 polymorphic markers identified, 560 (67%) were testcross markers heterozygous in one parent but null in the other (segregating 1:1), 206 (25%) were intercross dominant markers heterozygous in both parents (segregating 3:1), and the remaining 73 (9%) were 19 non-parental RAPD markers (segregating 1:1) and 54 codominant AFLP markers (segregating 1:1:1:1). A mixed set of the testcross markers, non-parental RAPD markers, and codominant AFLP markers was used to construct two linkage maps, one based on the P. deltoides (D) genome and the other based on P. euramericana (E). The two maps showed nearly complete coverage of the genome, spanning 3801 and 3452 cM, respectively. The availability of non-parental RAPD and codominant AFLP markers as orthologous genes allowed for a direct comparison of the rate of meiotic recombination between the two different parental species. Generally, the rate of meiotic recombination was greater for males than females in our interspecific poplar hybrids. The confounded effect of sexes and species causes the mean recombination distance of orthologous markers to be 11% longer for the father (P. euramericana; interspecific hybrid) than for the mother (P. deltoides; pure species). The linkage maps constructed and the interspecific poplar hybrid population in which clonal replicates for individual genotypes are available present a comprehensive foundation for future genomic studies and quantitative trait locus (QTL) identification.Key words: AFLP, Genetic map, poplar, RAPD, SSR.
    Type of Medium: Online Resource
    ISSN: 0831-2796 , 1480-3321
    URL: Article
    DOI: 10.1139/g02-013
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2002
    detail.hit.zdb_id: 2020635-5
    SSG: 12
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