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  • 1
    Online Resource
    Online Resource
    ART do not increase the risk of Y-chromosome microdeletion in 19 candidate genes at AZF regions
    CSIRO Publishing ; 2014
    In:  Reproduction, Fertility and Development Vol. 26, No. 6 ( 2014), p. 778-
    Details
    In: Reproduction, Fertility and Development, CSIRO Publishing, Vol. 26, No. 6 ( 2014), p. 778-
    Abstract: Y-chromosome microdeletions (YCMs) have been found at a much higher rate in infertile men than fertile controls. A specific deletion in the azoospermia factor locus (AZF) at Yq11 is significantly associated with male infertility. Whether assisted reproductive technology (ART) increases the risk of YCM in ART-derived offspring remains unclear. In this study the occurrence of YCM in 199 fathers and their 228 sons (Chinese, Han ethnicity), including 85 offspring conceived by IVF, 73 by intra-cytoplasmic sperm injection (ICSI) and 70 by natural conception, was investigated. Nineteen candidate genes related to YCM were analysed by multiplex ligation-dependent probe amplification. We identified one de novo YCM from 70 naturally-conceived offspring and none from 158 ART-conceived offspring and found no statistical significance between these two groups. There was no statistically-significant difference in the detection rate of the father’s Y-chromosome microdeletion group: IVF 10.7% (8/75), ICSI 3.2% (2/63), natural conception 8.2% (5/61). These results suggest that ART does not increase the risk of YCM in male offspring.
    Type of Medium: Online Resource
    ISSN: 1031-3613
    URL: Article
    DOI: 10.1071/RD13092
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2014
    SSG: 12
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  • 2
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    Chitosan and spermine enhance drought resistance in white clover, associated with changes in endogenous phytohormones and polyamines, and antioxidant metabolism
    CSIRO Publishing ; 2018
    In:  Functional Plant Biology Vol. 45, No. 12 ( 2018), p. 1205-
    Details
    In: Functional Plant Biology, CSIRO Publishing, Vol. 45, No. 12 ( 2018), p. 1205-
    Abstract: The interaction of chitosan and polyamines (PAs) could be involved mitigating drought stress in white clover (Trifolium repens L.). This research aimed to determine the effect of chitosan and PAs, and co-application of chitosan and PAs on improving drought tolerance associated with growth, phytohormones, polyamines and antioxidant metabolism. Plants were pretreated with or without 1 g L–1 chitosan, 0.5 mM spermine, or 1 g L–1 chitosan + 0.5 mM spermine, then subjected to drought induced by polyethylene glycol (PEG) 6000 (–0.5 MPa) in growth chambers for 14 days. Exogenous chitosan and spermine improved the level of PAs by regulating arginine decarboxylases, S-adenosyl methionine decarboxylase, copper-containing amine oxidase and polyamine oxidase activity, and expression of the genes encoding these enzymes under drought. Application of exogenous chitosan improved ABA content under normal and drought conditions. In addition, chitosan and spermine significantly enhanced the levels of cytokinin and GA, but reduced IAA levels during drought stress. Exogenous chitosan and spermine improved antioxidant defence, including enzyme activity, gene expression and the content of ascorbate and glutathione compounds, leading to a decline in superoxide anion radicals, H2O2 and malondialdehyde, effectively mitigating drought-induced oxidative damage. Other protective metabolites, such as total phenols and flavonoids, increased considerably under application of chitosan and spermine. These results suggest that chitosan-induced drought tolerance could be involved in PA metabolism, changes in endogenous phytohormones and antioxidant defence in white clover. Co-application of chitosan and spermine was more effective than either chitosan or spermine alone in mitigating drought stress.
    Type of Medium: Online Resource
    ISSN: 1445-4408
    URL: Article
    DOI: 10.1071/FP18012
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2018
    SSG: 12
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  • 3
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    Andrographolide disrupts meiotic maturation by blocking cytoskeletal reorganisation and decreases the fertilisation potential of mouse oocytes
    CSIRO Publishing ; 2017
    In:  Reproduction, Fertility and Development Vol. 29, No. 12 ( 2017), p. 2336-
    Details
    In: Reproduction, Fertility and Development, CSIRO Publishing, Vol. 29, No. 12 ( 2017), p. 2336-
    Abstract: Andrographolide (AG) is a diterpenoid lactone isolated from the stem and leaves of Andrographis paniculata Nees that is used for the effective treatment of infectious diseases in Asian countries. Previous studies have reported adverse effects of AG on female fertility in rodents; however, the underlying mechanisms are unknown. The aim of the present study was to investigate the effects of AG on the IVM of mouse oocytes and their fertilisation potential. Immature oocytes incubated for 6, 14 or 24 h in medium containing 5, 10 or 20 μM AG showed time- and dose-dependent decreases in maturation rates compared with the control group. Immunostaining revealed that AG exposure disrupted spindle organisation and migration, as well as actin cap formation and cytokinesis. Furthermore, most oocytes exposed to 20 μM AG underwent apoptosis, and the few oocytes exposed to 5 or 10 μM AG that reached MII exhibited lower fertilisation rates after intracytoplasmic sperm injection. The findings of the present study suggest that AG may disrupt mouse oocyte meiotic maturation by blocking cytoskeletal reorganisation, and may thus have an adverse effect on female fertility.
    Type of Medium: Online Resource
    ISSN: 1031-3613
    URL: Article
    DOI: 10.1071/RD16343
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2017
    SSG: 12
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  • 4
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    In-situ sampling of available calcium using diffusive gradients in thin-films technique based on benzo-crown ether-functionalised silica as the binding agent
    CSIRO Publishing ; 2018
    In:  Environmental Chemistry Vol. 15, No. 4 ( 2018), p. 205-
    Details
    In: Environmental Chemistry, CSIRO Publishing, Vol. 15, No. 4 ( 2018), p. 205-
    Abstract: Environmental contextLow availability of calcium (Ca2+) in soils is one of the major factors in Ca2+ deficiency of plants and physiological plant disorders. A device based on functionalised silica was developed for in-situ measurement of the available Ca2+ in soils. Application of the proposed device to measure available Ca2+ may help to develop and improve agricultural practices. AbstractCalcium is an ion of particular interest due to its importance in plant nutrition and soil structure. A novel device of diffusion gradients in thin-films (DGT) based on the benzo-crown ether-functionalised silica (BCES) as the binding agent and the polyethersulfone (PES) membrane as diffusive layer (BCES-DGT) was developed for in-situ sampling of available calcium (Ca2+) in freshwater and soil samples. The performance characteristics of the BCES-DGT device were assessed. The BCES was prepared using the sol-gel process and characterised by Fourier transform infrared spectroscopy, scanning electron microscopy, thermal gravimetric analysis and N2 adsorption–desorption. Results evinced that BCES was obtained successfully with a rough wrinkled surface and good specific surface area of 111.3 m2 g−1. The diffusion coefficient of Ca2+ ions in PES membrane was found to be 1.23 × 10−6 cm2 s−1 at 25 °C and was independent of pH in the range of 3–10 and ionic strength (as pNaCl) from 1 to 3. The high binding capacity of BCES binding gel for Ca2+ ions was determined to be 9822.4 ± 452.9 μg Ca2+/disk and was conducive to the deployment of long-term or high concentration. The BCES-DGT device can accurately measure the concentrations of Ca2+ over wide ranges of ionic strengths (1–3 as pNaCl) and pH (5–10). There was no significant interference on the uptake of Ca2+ by the BCES-DGT device at the tolerance limits up to 500 for Mg2+, Li+, HCO3−, H2PO4−, NO3− and SO42−, 250 for Sr2+, 2000 for K+ and 50 for fulvic acid. The results from BCES-DGT device were in excellent agreement with those measured directly using ion selective electrode in several water and soil samples. Field application in river water indicated that a good agreement was obtained between BCES-DGT value and mean grab sample measurements of Ca2+ and that the relative standard deviation of BCES-DGT measurement (4.7 %) was superior to that of grab sample measurements (13.5 %), suggesting that BCES-DGT was reliable for in-situ sampling and measurement of available Ca2+ with good accuracy and precision.
    Type of Medium: Online Resource
    ISSN: 1448-2517
    URL: Article
    DOI: 10.1071/EN17228
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2018
    detail.hit.zdb_id: 2150372-2
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  • 5
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    Syntheses, Crystal Structures, and Properties of Six Coordination Complexes Based on a Newly Designed Mercapto-thiadiazole Ligand
    CSIRO Publishing ; 2014
    In:  Australian Journal of Chemistry Vol. 67, No. 2 ( 2014), p. 241-
    Details
    In: Australian Journal of Chemistry, CSIRO Publishing, Vol. 67, No. 2 ( 2014), p. 241-
    Abstract: Six coordination complexes were solvothermally synthesised: a 3D framework [Cd(tmtt)2]n (1), 2D architectures [Zn(tmtt)2] n (2) and [Pb(tmtt)2]n (3), 1D chain structures [Ni(tmtt)2·(H2O)2] n (4) and [Co(tmtt)2·(H2O)2]n (5), and a mononuclear structure [Hg(tmtt)2] (6). The complexes, based on self-assembly of different metal ions with a newly designed mercapto-thiadiazole ligand tmttH (tmttH = 5-[(1H-1,2,4-triazol-1-yl)methyl]-1,3,4-thiadiazole-2(3H)-thione), were characterised by single crystal X-ray diffraction analyses. Crystal structure analyses reveal that complex 1 exhibits a four-fold interpenetrating 3D framework with {64.82} topology based on two kinds of right-handed single-helical chains, 2 displays a bilayer structure, 3 presents a crown-shaped network, 4 and 5 show 1D double–helical chain structures, and 6 is a mononuclear structure. Moreover, the thermal stabilities of crystalline samples 1–6 have been investigated, and the luminescent properties of complexes 1, 2, 3, 6, and the free ligand have been studied. The results of photoluminescent measurements illustrate that 2 and 3 may serve as excellent candidates for potential photoactive materials.
    Type of Medium: Online Resource
    ISSN: 0004-9425
    URL: Article
    DOI: 10.1071/CH13417
    RVK:
    VA 2470
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2014
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  • 6
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    Magnetic Fe3O4 Nanoparticles as New, Efficient, and Reusable Catalysts for the Synthesis of Quinoxalines in Water
    CSIRO Publishing ; 2010
    In:  Australian Journal of Chemistry Vol. 63, No. 8 ( 2010), p. 1290-
    Details
    In: Australian Journal of Chemistry, CSIRO Publishing, Vol. 63, No. 8 ( 2010), p. 1290-
    Abstract: A novel, environmentally friendly procedure has been developed for the synthesis of quinoxaline derivatives in the presence of magnetic Fe3O4 nanoparticles. The reaction between 1,2-diamines and 1,2-dicarbonyl compounds was carried out in water to afford quinoxaline derivatives in high yield. The catalyst can be recovered by the use of an external magnet and reused for five cycles with almost consistent activity.
    Type of Medium: Online Resource
    ISSN: 0004-9425
    URL: Article
    DOI: 10.1071/CH09532
    RVK:
    VA 2470
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2010
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  • 7
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    Nutritional effects pre-weaning on growth performance, carcass traits and meat quality of pigs
    CSIRO Publishing ; 2020
    In:  Animal Production Science Vol. 60, No. 7 ( 2020), p. 978-
    Details
    In: Animal Production Science, CSIRO Publishing, Vol. 60, No. 7 ( 2020), p. 978-
    Abstract: The objective of the present study was to investigate the effect of nutritional restriction during the suckling period on growth performance, carcass traits and meat quality of fattening pigs. A one-way experimental design was used. In total, 24 male pigs of normal birthweight (1.54 ± 0.05 kg) were randomly allocated at 7 days of age to three groups: control, fed ad libitum; 30% nutritional restriction (Re30%); and 60% nutritional restriction (Re60%). The Re30% and Re60% groups showed decreased average daily gain, average daily feed intake and feed conversion ratio from Day 7 to Day 28. After Day 28, differences in these parameters were observed only in the Re60% group relative to the control. With regard to hormone levels, the Re60% group showed decreased serum concentration of insulin-like growth factor-1 at Day 28 and increased serum concentration of growth hormone at Day 147. Furthermore, the Re60% group had decreased carcass weight, ham weight and dressing percentage, and increased carcass lean percentage relative to the control, as well as lower cross-sectional area and myofibre diameter of muscle. The Re60% group had lower levels of myosin heavy chain (MyHC) IIx and MyHC IIb mRNA and a higher percentage of MyHC I and MyHC IIa mRNA in longissimus dorsi muscle than the Re30% group. In conclusion, nutritional restriction during the suckling period decreased weaning weight, with post-weaning growth performance, carcass traits and myofibre type affected in the Re60% group rather than Re30% group.
    Type of Medium: Online Resource
    ISSN: 1836-0939
    URL: Article
    DOI: 10.1071/AN18368
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2020
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  • 8
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    Transcriptome profiling of peanut (Arachis hypogaea) gynophores in gravitropic response
    CSIRO Publishing ; 2013
    In:  Functional Plant Biology Vol. 40, No. 12 ( 2013), p. 1249-
    Details
    In: Functional Plant Biology, CSIRO Publishing, Vol. 40, No. 12 ( 2013), p. 1249-
    Abstract: Peanut (Arachis hypogaea L.) produces flowers aerially, but the fruit develops underground. This process is mediated by the gynophore, which always grows vertically downwards. The genetic basis underlying gravitropic bending of gynophores is not well understood. To identify genes related to gynophore gravitropism, gene expression profiles of gynophores cultured in vitro with tip pointing upward (gravitropic stimulation sample) and downward (control) at both 6 and 12 h were compared through a high-density peanut microarray. After gravitropic stimulation, there were 174 differentially expressed genes, including 91 upregulated and 83 downregulated genes at 6 h, and 491 differentially expressed genes including 129 upregulated and 362 downregulated genes at 12 h. The differentially expressed genes identified were assigned to 24 functional categories. Twenty pathways including carbon fixation, aminoacyl-tRNA biosynthesis, pentose phosphate pathway, starch and sucrose metabolism were identified. The quantitative real-time PCR analysis was performed for validation of microarray results. Our study paves the way to better understand the molecular mechanisms underlying the peanut gynophore gravitropism.
    Type of Medium: Online Resource
    ISSN: 1445-4408
    URL: Article
    DOI: 10.1071/FP13075
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2013
    SSG: 12
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  • 9
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    Online Resource
    Synthesis, Structures, and Properties of Polynuclear Silver(I) Complexes Containing Tetra-Phosphine Ligand with Ag⋅⋅⋅C Interactions
    CSIRO Publishing ; 2016
    In:  Australian Journal of Chemistry Vol. 69, No. 3 ( 2016), p. 336-
    Details
    In: Australian Journal of Chemistry, CSIRO Publishing, Vol. 69, No. 3 ( 2016), p. 336-
    Abstract: Reaction of AgNO3 and N,N,N′,N′-tetrakis((diphenylphosphino)methyl)benzene-1,4-diamine (pbaa) with sodium N-ethyldithiocarbamate (Na(Etdtc)) in CH3CN/toluene and CH3CN/DMF solvents produced two Ag4S4-based coordination complexes [Ag4(pbaa)(µ-κ1S,κ2S-Etdtc)4] (1) and [Ag4(pbaa)(µ-κ1S,κ2S-Etdtc)2(µ-κ1S,κ1S-Etdtc)2] (2). Structural analysis shows that the Ag4S4 cores in 1 are interconnected by one pbaa ligand in a tetradentate mode and four Etdtc– anions in a µ-κ1S,κ2S mode to form a three-layer conformation, whereas the Ag4S4 cores in 2 are linked by ligands pbaa (the tetradentate mode) and Etdtc– (the µ-κ1S,κ1S and µ-κ1S,κ2S modes) to yield the other type of three-layer conformation. In addition, in different solvent systems, the Ag atoms also form different types of weak Ag···C interactions with Ag···C distances of 3.297–3.344 Å in 1 and 3.237–3.416 Å in 2. The emission spectrum of complex 1 in DMF solution displays a broad orange–red emission peak at 518 nm, which may be assigned to the ligand-to-metal charge transfer transition.
    Type of Medium: Online Resource
    ISSN: 0004-9425
    URL: Article
    DOI: 10.1071/CH15413
    RVK:
    VA 2470
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2016
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  • 10
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    Transcriptome identification of the resistance-associated genes (RAGs) to Aspergillus flavus infection in pre-harvested peanut (Arachis hypogaea)
    CSIRO Publishing ; 2013
    In:  Functional Plant Biology Vol. 40, No. 3 ( 2013), p. 292-
    Details
    In: Functional Plant Biology, CSIRO Publishing, Vol. 40, No. 3 ( 2013), p. 292-
    Abstract: Pre-harvest aflatoxin contamination caused by Aspergillus favus is a major concern in peanut. However, little is known about the resistance mechanism, so the incorporation of resistance into cultivars with commercially-acceptable genetic background has been slowed. To identify resistance-associated genes potentially underlying the resistance mechanism, we compared transcriptome profiles in resistant and susceptible peanut genotypes under three different treatments: well watered, drought stress and both A. flavus and drought stress using a customised NimbleGen microarray representing 36 158 unigenes. Results showed that the profile of differentially expressed genes (DEGs) displayed a similar pattern of distribution among the functional classes between resistant and susceptible peanuts in response to drought stress. Under A. flavus infection with drought stress, a total of 490 unigenes involved in 26 pathways were differentially expressed in the resistant genotype YJ1 uniquely responding to A. flavus infection, in which 96 DEGs were related to eight pathways: oxidation reduction, proteolysis metabolism, coenzyme A biosynthesis, defence response, signalling, oligopeptide transport, transmembrane transport and carbohydrate biosynthesis/metabolism. Pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database showed that eight networks were significantly associated with resistance to A. flavus infection in resistant genotype YJ1 compared with susceptible Yueyou7. To validate microarray analysis, 15 genes were randomly selected for real-time RT–PCR analysis. The results provided in this study may enhance our understanding of the pre-harvest peanut–A. flavus interaction and facilitate to develop aflatoxin resistant peanut lines in future breeding programs.
    Type of Medium: Online Resource
    ISSN: 1445-4408
    URL: Article
    DOI: 10.1071/FP12143
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2013
    SSG: 12
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