In:
Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 41, No. 1-2 ( 1986-2-1), p. 215-221
Abstract:
Experiments with cerulenin-inhibited cultures of 5. violaceoruber showed conversion of dihydrogranaticin (II) into granaticin (I), but not vice versa, confirming an earlier conclusion that II is the biosynthetic precursor of I. Feeding of CH 3 13 C 18 O 2 Na followed by 13 C-NMR analysis of the product by the 18 O shift method indicated the expected incorporation of 18 O at carbons 1,11 and 13 of I and showed that the oxygen of the pyran ring originates from C-3 and not from C-15. Analysis of I biosynthesized from 13 C 2 H 3 COONa by 13 C{ 1 H, 2 H} triple resonance NMR spectroscopy showed the incorporation of one atom of deuterium each at C-2 and C-4. C-16 carried a maximum of 2, not 3, atoms of deuterium. These results are discussed in terms of biosynthetic mechanisms.
Type of Medium:
Online Resource
ISSN:
1865-7125
,
0939-5075
DOI:
10.1515/znc-1986-1-232
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1986
detail.hit.zdb_id:
2078107-6
SSG:
12
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