In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 97, No. 24 ( 2000-11-21), p. 13015-13020
Abstract:
The SWI/SNF family of chromatin-remodeling complexes facilitates
gene expression by helping transcription factors gain access to their targets in chromatin. SWI/SNF and Rsc are distinctive members of this
family from yeast. They have similar protein components and catalytic activities but differ in biological function. Rsc is required for cell
cycle progression through mitosis, whereas SWI/SNF is not. Human complexes of this family have also been identified, which have often
been considered related to yeast SWI/SNF. However, all human subunits identified to date are equally similar to components of both SWI/SNF
and Rsc, leaving open the possibility that some or all of the human complexes are rather related to Rsc. Here, we present evidence that the
previously identified human SWI/SNF-B complex is indeed of the Rsc type. It contains six components conserved in both Rsc and SWI/SNF.
Importantly, it has a unique subunit, BAF180, that harbors a distinctive set of structural motifs characteristic of three components
of Rsc. Of the two mammalian ATPases known to be related to those in the yeast complexes, human SWI/SNF-B contains only the homolog that
functions like Rsc during cell growth. Immunofluorescence studies with a BAF180 antibody revealed that SWI/SNF-B localizes at the
kinetochores of chromosomes during mitosis. Our data suggest that SWI/SNF-B and Rsc represent a novel subfamily of chromatin-remodeling
complexes conserved from yeast to human, and could participate in cell division at kinetochores of mitotic chromosomes.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.240208597
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2000
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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