In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 61, No. 12 ( 2011-12-01), p. 2901-2906
Abstract:
A Gram-stain-negative, motile bacterium with two lateral flagella, designated strain B201 T , was isolated from beach sand from the Taean coast in South Korea. Cells were ovoid rods and positive for catalase and oxidase. Growth of strain B201 T was observed between 15 and 45 °C (optimum, 30 °C) and between pH 5.0 and 9.0 (optimum, pH 6.0–7.5). Strain B201 T contained ubiquinone Q-8 as the major isoprenoid quinone, but MK-6 was also present as a minor quinone. The major fatty acids of strain B201 T were C 17 : 0 cyclo, C 16 : 0 , summed feature 2 (iso-C 16 : 1 I/C 14 : 0 3-OH and/or C 12 : 0 ALDE), C 12 : 0 and C 19 : 0 cyclo ω8 c . The major cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and three aminolipids. The genomic DNA G+C content was 53.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a phyletic lineage with Pusillimonas ginsengisoli DCY25 T within the genus Pusillimonas . Strain B201 T was most closely related to P. ginsengisoli DCY25 T and Pusillimonas soli MJ07 T with similarities of 98.6 and 97.5 %, respectively. However, DNA–DNA relatedness values of strain B201 T with P. ginsengisoli DCY25 T and P. soli MJ07 T were 30.2±5.4 and 4.9±1.8 %, respectively. On the basis of chemotaxonomic data and molecular properties, strain B201 T represents a novel species of the genus Pusillimonas , for which the name Pusillimonas harenae sp. nov. is proposed; the type strain is B201 T ( = KACC 14927 T = JCM 16917 T ). An emended description of the genus Pusillimonas is given.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.029892-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2011
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
Permalink