In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_8 ( 2014-08-01), p. 2812-2818
Abstract:
A Gram-staining-negative bacterium, strain DSG-S4-2 T , was isolated from Dasugan Lake, a saline lake (salinity 3.1 %, w/v) in Qaidam basin, Qinghai, China and its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S4-2 T were non-spore-forming rods, 0.5–0.8 µm wide and 1.2–3.8 µm long and motile by means of a single polar flagellum. Strain DSG-S4-2 T was strictly heterotrophic and aerobic, catalase-positive and oxidase-negative. PufLM and coxL genes were present, bacteriochlorophyll a (BChl a ) and a carotenoid pigment were produced. Growth was observed in the presence of 0–8.0 % (w/v) NaCl (optimum, 1.0–2.0 %), at 20–40 °C (optimum, 35 °C) and pH 6.5–10.5 (optimum, pH 7.5–8.0). Strain DSG-S4-2 T contained Q-10 as the sole respiratory quinone. The polar lipids contained two aminolipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, sulphoquinovosyldiacylglyceride, phosphatidylcholine and some unknown phospholipids, like the other members of the genus Roseibium . The predominant fatty acid ( 〉 70 %) was summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ). The DNA G+C content was 61.4 mol% (determined from melting temperature). Phylogenetic trees (neighbour-joining, maximum-likelihood and maximum-parsimony) based on 16S rRNA gene sequences showed that strain DSG-S4-2 T was associated with the members of the genus Roseibium , with highest 16S rRNA gene sequence similarity to Roseibium denhamense OCh 254 T (96.3 %) and Roseibium hamelinense OCh 368 T (96.3 %). Based on the data presented above, it is concluded that strain DSG-S4-2 T represents a novel species of the genus Roseibium , for which the name Roseibium aquae sp. nov. is proposed. The type strain is DSG-S4-2 T ( = CGMCC 1.12426 T = JCM 19310 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.065508-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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