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  • 1
    Online Resource
    Online Resource
    Microbiology Society ; 2014
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 64, No. Pt_2 ( 2014-02-01), p. 469-474
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_2 ( 2014-02-01), p. 469-474
    Abstract: A Gram-stain-negative, strictly aerobic and heterotrophic bacterial strain, designed strain D1 T , was isolated from a recirculating mariculture system in Tianjin, China. Its taxonomic position was determined using a polyphasic approach. Cells of strain D1 T were non-flagellated short rods, 0.3–0.5 µm wide and 0.5–1.0 µm long. Growth was observed at 15–30 °C (optimum, 25 °C), at pH 5.5–9.0 (optimum, pH 6.5–7.0) and in the presence of 1–8 % (w/v) NaCl (optimum, 2–3 %). Cells contained carotenoid pigments but not flexirubin-type pigments. Strain D1 T contained MK-6 as the sole menaquinone and phosphatidylethanolamine (PE) as the sole phospholipid and four unidentified lipids. The major cellular fatty acids ( 〉 10 %) were iso-C 15 : 0 (23.2 %), iso-C 17 : 0 3-OH (15.2 %), C 16 : 1 ω7 c /C 16 : 1 ω6 c (14.3 %), iso-C 15 : 0 3-OH (13.5 %) and iso-C 15 : 1 G (10.8 %). 16S rRNA gene sequence analyses indicated that strain D1 T belonged to the family Flavobacteriaceae and showed closest phylogenetic relationship to the genus Lutibacter , with highest sequence similarity to Lutibacter aestuarii MA-My1 T (92.2 %). The DNA G+C content of strain D1 T was 35.9 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain D1 T was considered to represent a novel species in a new genus of the family Flavobacteriaceae , for which the name Wenyingzhuangia marina gen. nov., sp. nov. is proposed. The type strain of the type species is D1 T ( = CGMCC 1.12162 T  = JCM 18494 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2014
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    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 2
    In: Xenobiotica, Informa UK Limited, Vol. 42, No. 8 ( 2012-08), p. 709-718
    Type of Medium: Online Resource
    ISSN: 0049-8254 , 1366-5928
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2012
    detail.hit.zdb_id: 1485113-1
    SSG: 12
    SSG: 15,3
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  • 3
    Online Resource
    Online Resource
    Microbiology Society ; 2013
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 63, No. Pt_12 ( 2013-12-01), p. 4489-4494
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_12 ( 2013-12-01), p. 4489-4494
    Abstract: A Gram-positive, strictly aerobic and heterotrophic, non-spore-forming actinobacterium (strain B2 T ) isolated from a recirculating aquaculture system was studied for its taxonomic position. Strain B2 T formed a rudimentary substrate-mycelium that fragmented into short rod-shaped to coccoid cells (0.5 µm×0.5–2.2 µm or 0.5–1.0 µm in diameter). Colonies were yellow, smooth, circular and 1.5–2.0 mm in diameter after incubation on TSA for 3 days at 30 °C. Strain B2 T grew at 20–40 °C (optimal, 30 °C) and pH 5.5–9.5 (optimal, 6.5–7.0) and in the presence of 0–9 % (w/v) NaCl (optimal, 1 %). The predominant menaquinone of strain B2 T was MK-8(H 4 ). The cell-wall peptidoglycan of strain B2 T contained the amino acids ornithine, glutamic acid, alanine, glycine and aspartic acid. The major polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were iso-C 15 : 0 , iso-C 16 : 0 and summed feature 9. Its DNA G+C content was 68.3 mol% ( T m ). Analysis of 16S rRNA gene sequences indicated that strain B2 T was related phylogenetically to members of the genus Ornithinimicrobium with highest similarity (96.6 %) to Ornithinimicrobium kibberense DSM 17687 T , followed by Ornithinimicrobium humiphilum DSM 12362 T (96.3 %), Ornithinimicrobium pekingense LW6 T (96.2 %) and Ornithinimicrobium murale 01-Gi-040 T (94.8 %). On basis of phenotypic, chemotaxonomic and phylogenetic data, it was concluded that strain B2 T represents a novel species of the genus Ornithinimicrobium , for which the name Ornithinimicrobium tianjinense sp. nov. is proposed. The type strain is B2 T ( = CGMCC 1.12160 T  = JCM 18464 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2013
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Microbiology Society ; 2014
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 64, No. Pt_10 ( 2014-10-01), p. 3411-3416
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_10 ( 2014-10-01), p. 3411-3416
    Abstract: A Gram-stain-negative bacterium, strain TS-T11 T , was isolated from Tuosu lake, a saline lake (salinity 5.4 %, w/v) in the Qaidam basin, Qinghai province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain TS-T11 T were non-spore-forming rods, 0.6–0.8 µm wide and 0.8–2.2 µm long, and motile by means of a single polar flagellum. Strain TS-T11 T was strictly heterotrophic and aerobic. Cells were positive for catalase and oxidase. Growth was observed in the presence of 0.5–11.0 % (w/v) NaCl (optimum 4.0–6.0 %), at 4–40 °C (optimum 30–35 °C) and at pH 6.0–10.5 (optimum pH 7.5–8.5). Strain TS-T11 T contained iso-C 15 : 0 , iso-C 17 : 0 and iso-C 17 : 1 ω9 c as the predominant fatty acids ( 〉 10 %). The major respiratory quinone was Q-8. The polar lipids consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and nine uncharacterized phospholipids. The G+C content of genomic DNA was 46.8 mol% ( T m ). Phylogenetic trees based on 16S rRNA gene sequences showed that strain TS-T11 T was associated with the genus Idiomarina , and showed highest 16S rRNA gene sequence similarity to Idiomarina aestuarii KYW314 T (97.4 %) and Idiomarina salinarum ISL-52 T (97.4 %). DNA–DNA relatedness of strain TS-T11 T to I. aestuarii JCM 16344 T and I. salinarum DSM 21900 T was 22.2±2.4 and 11.5±1.6 %, respectively. Based on the data presented above, it was concluded that strain TS-T11 T represents a novel species of the genus Idiomarina , for which the name Idiomarina planktonica sp. nov. is proposed. The type strain is TS-T11 T ( = CGMCC 1.12458 T  = JCM 19263 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2014
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 5
    In: Parasites & Vectors, Springer Science and Business Media LLC, Vol. 5, No. 1 ( 2012-12)
    Type of Medium: Online Resource
    ISSN: 1756-3305
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2012
    detail.hit.zdb_id: 2409480-8
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  • 6
    Online Resource
    Online Resource
    Microbiology Society ; 2014
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 64, No. Pt_8 ( 2014-08-01), p. 2812-2818
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_8 ( 2014-08-01), p. 2812-2818
    Abstract: A Gram-staining-negative bacterium, strain DSG-S4-2 T , was isolated from Dasugan Lake, a saline lake (salinity 3.1 %, w/v) in Qaidam basin, Qinghai, China and its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S4-2 T were non-spore-forming rods, 0.5–0.8 µm wide and 1.2–3.8 µm long and motile by means of a single polar flagellum. Strain DSG-S4-2 T was strictly heterotrophic and aerobic, catalase-positive and oxidase-negative. PufLM and coxL genes were present, bacteriochlorophyll a (BChl a ) and a carotenoid pigment were produced. Growth was observed in the presence of 0–8.0 % (w/v) NaCl (optimum, 1.0–2.0 %), at 20–40 °C (optimum, 35 °C) and pH 6.5–10.5 (optimum, pH 7.5–8.0). Strain DSG-S4-2 T contained Q-10 as the sole respiratory quinone. The polar lipids contained two aminolipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, sulphoquinovosyldiacylglyceride, phosphatidylcholine and some unknown phospholipids, like the other members of the genus Roseibium . The predominant fatty acid ( 〉 70 %) was summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ). The DNA G+C content was 61.4 mol% (determined from melting temperature). Phylogenetic trees (neighbour-joining, maximum-likelihood and maximum-parsimony) based on 16S rRNA gene sequences showed that strain DSG-S4-2 T was associated with the members of the genus Roseibium , with highest 16S rRNA gene sequence similarity to Roseibium denhamense OCh 254 T (96.3 %) and Roseibium hamelinense OCh 368 T (96.3 %). Based on the data presented above, it is concluded that strain DSG-S4-2 T represents a novel species of the genus Roseibium , for which the name Roseibium aquae sp. nov. is proposed. The type strain is DSG-S4-2 T ( = CGMCC 1.12426 T  = JCM 19310 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2014
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Microbiology Society ; 2010
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 60, No. 2 ( 2010-02-01), p. 417-421
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 60, No. 2 ( 2010-02-01), p. 417-421
    Abstract: A Gram-stain-negative, heterotrophic, aerobic, non-spore-forming and non-motile bacterial strain, designated LM5 T , was isolated from activated sludge from a sequencing batch reactor for the treatment of effluents contaminated by malachite green. The taxonomy of strain LM5 T was studied by phenotypic and phylogenetic methods. Strain LM5 T formed orange colonies on R2A and YP plates. Cells were rods, 0.4–0.6 μm in diameter and 0.8–1.2 μm in length. Growth occurred at 10–35 °C (optimum, 20–25 °C), at pH 5.5–9.5 (optimum, pH 6.5–7.5) and in the presence of 0–2 % (w/v) NaCl (optimum, 0.5 %). Oxidase and catalase activities were present. Flexirubin-type pigments were present, but extracellular glycans were absent. MK-6 was the major respiratory quinone. The major fatty acids were iso-C 15 : 0 (28.3 %) and iso-C 17 : 1 ω 9 c (13.8 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LM5 T was a member of the genus Flavobacterium with highest sequence similarity to Flavobacterium soli DS-6 T (93.2 %) and Flavobacterium lindanitolerans IP-10 T (92.9 %). Together with F. lindanitolerans IP-10 T , strain LM5 T formed a distinct lineage in the phylogenetic tree. The DNA G+C content was 52±0.6 mol% (HPLC), which is significantly higher than that of other species of the genus Flavobacterium (30–41 mol%). Based on phylogenetic and phenotypic evidence, strain LM5 T is considered to represent a novel species of the genus Flavobacterium , for which the name Flavobacterium caeni sp. nov. is proposed; the type strain is LM5 T (=CGMCC 1.7031 T =NBRC 104239 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2010
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Microbiology Society ; 2013
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 63, No. Pt_4 ( 2013-04-01), p. 1557-1561
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_4 ( 2013-04-01), p. 1557-1561
    Abstract: A Gram-staining-negative, non-endospore-forming, non-flagellated, non-motile and rod-shaped bacterium, designated strain LM2-5 T , was isolated from activated sludge in a sequencing batch reactor used for the treatment of triphenylmethane dye effluent. The taxonomy of strain LM2-5 T was studied by phenotypic, chemotaxonomic and phylogenetic methods. Strain LM2-5 T was aerobic, heterotrophic and positive for oxidase but negative for catalase activity. It grew at 16–37 °C (optimum 25 °C) and at pH 5.0–8.5 (optimum between pH 6.5 and pH 7.0). NaCl was not obligatory for growth but was tolerated at concentrations up to 2.0 % (w/v) NaCl. The novel strain formed yellow colonies on trypticase soy agar. Cells of strain LM2-5 T were rods that measured 0.3–0.5 µm in width and 3.0–5.0 µm in length. The predominant respiratory quinone was Q-8. The major fatty acids were iso-C 15 : 0 and iso-C 17 : 1 ω9 c . The genomic DNA G+C content was 66.7 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, strain LM2-5 T clustered with members of the genus Dokdonella and appeared most closely related to Dokdonella koreensis DS-123 T (96.4 % sequence similarity), Dokdonella fugitiva A3 T (96.1 %), Dokdonella soli KIS28-6 T (95.7 %) and Dokdonella ginsengisoli Gsoil 191 T (95.7 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain LM2-5 T was considered to represent a novel species of the genus Dokdonella , for which the name Dokdonella immobilis is proposed. The type strain is LM2-5 T ( = CGMCC 1.7659 T  = JCM 15763 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2013
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 9
    In: Parasites & Vectors, Springer Science and Business Media LLC, Vol. 4, No. 1 ( 2011-12)
    Type of Medium: Online Resource
    ISSN: 1756-3305
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2011
    detail.hit.zdb_id: 2409480-8
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  • 10
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2011
    In:  Plant and Soil Vol. 346, No. 1-2 ( 2011-9), p. 189-199
    In: Plant and Soil, Springer Science and Business Media LLC, Vol. 346, No. 1-2 ( 2011-9), p. 189-199
    Type of Medium: Online Resource
    ISSN: 0032-079X , 1573-5036
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2011
    detail.hit.zdb_id: 1478535-3
    detail.hit.zdb_id: 208908-7
    SSG: 12
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