In:
ELECTROPHORESIS, Wiley, Vol. 31, No. 9 ( 2010-05), p. 1572-1582
Abstract:
Phenytoin is a widely used anti‐seizure agent, and a good correlation is observed between its concentration in plasma and the clinical effect. We developed a selective CE with UV detection at 200 nm for analysis of free and total levels of phenytoin in human plasma based on MEKC. A sample pretreatment by liquid–liquid extraction with ethyl acetate for determination of total level of phenytoin and serum ultrafiltrate was prepared by ultrafiltration technique (ultrafiltration membrane 30 kDa; 2000 g , 10 min) for determination of free level of phenytoin and subsequent quantification by MEKC was used. MEKC was performed in Tris buffer (25 mM; pH 10.5) containing SDS (180 mM) and EG (13%) as BGE. Hydrodynamic injection for phenytoin determination (0.5 psi 5 s for total level, 2 psi 5 s for free level) was used to introduce samples. The separation voltage was set at 20 kV. Data obtained by MEKC were compared with the results by a validated HPLC method. The MEKC assay of phenytoin exhibited a very good correlation with respect to HPLC by Bland–Altman method. The equations for the Passing–Bablok regression line were as follows: for total level: MEKC=1.0143×HPLC+0.0976, r 2 =1; for free level: MEKC=1.0013×HPLC–0.0016, r 2 =1. The proposed method was applied successfully to monitor free and total levels of phentoin in 20 patients with seizures.
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.200900768
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
1475486-1
SSG:
12
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