In:
Genes to Cells, Wiley, Vol. 21, No. 11 ( 2016-11), p. 1150-1163
Abstract:
Serum endonucleases are essential for degrading the chromatin released from dead cells and preventing autoimmune diseases such as systemic lupus erythematosus. Serum DN ase I is known as the major endonuclease, but recently, another endonuclease, DN ase γ/ DN ase I‐like 3, gained attention. However, the precise role of each endonuclease, especially that of DN ase γ, remains unclear. In this study, we distinguished the activities of DN ase γ from those of DN ase I in mouse serum and concluded that both cooperated in degrading DNA during necrosis: DN ase γ functions as the primary chromatolytic activity, causing internucleosomal DNA fragmentation, and DN ase I as the secondary one, causing random DNA digestion for its complete degradation. These results were confirmed by two in vivo experimental mouse models, in which necrosis was induced, acetaminophen‐induced hepatic injury and streptozotocin‐induced β‐cell necrosis models. We also determined that DN ase γ functions as a backup endonuclease for caspase‐activated DN ase ( CAD ) in the secondary necrosis phase after γ‐ray‐induced apoptosis in vivo .
Type of Medium:
Online Resource
ISSN:
1356-9597
,
1365-2443
DOI:
10.1111/gtc.2016.21.issue-11
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2020308-1
SSG:
12
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