In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 71, No. 3 ( 2019-06-06)
Abstract:
Two strains (NLN63 T and NLN82) of Gram-stain-negative, oxidase- and catalase-positive, bacilli-shaped organisms were isolated from the faecal samples of two separate Rattus norvegicus in Baisha county of Hainan Province, Southern PR China. Phylogenetic analysis based on the near full-length 16S rRNA sequences revealed that strain NLN63 T belongs to the genus Pelistega , having maximum similarity to Pelistega suis CCUG 64465 T (97.1 %), Pelistega europaea CCUG 39967 T (96.2 %) and Pelistega indica DSM 27484 T (96.2 %), respectively. The phylogenomic tree built on 553 core genes from genomes of 20 species in the genus Pelistega and other adjacent genera further confirmed that strains NLN63 T and NLN82 form a distinct subline and exhibit specific phylogenetic affinity with P. europaea CCUG 39967 T . In digital DNA–DNA hybridization analyses, strain NLN63 T showed low estimated DNA reassociation values (21.4–22.6 %) with the type strains of the species in the genus Pelistega . The DNA G+C contents of strains NLN63 T and NLN82 were 37.3 and 37.1 mol%, respectively. Strain NLN63 T had a unique MALDI-TOF MS profile, contained Q-8 as the major quinone and C 16 : 0 , summed feature 8 (C 18 : 1 ω 7 c /C 18 : 1 ω 6 c or both) and summed feature 3 (C 16 : 1 ω 7 c /C 16 : 1 ω 6 c or both) as the dominant fatty acids. Based upon these polyphasic characterization data obtained from the present study, a novel species of the genus Pelistega , Pelistega ratti sp. nov., is proposed with NLN63 T (=GDMCC 1.1697 T =JCM 33788 T ) as the type strain.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004733
Language:
English
Publisher:
Microbiology Society
Publication Date:
2019
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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