In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 2 ( 2020-02-01), p. 766-772
Abstract:
A novel actinomycete, designated strain NEAU-Ht49 T , was isolated from sea sand sampled in Sanya and characterized by using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-Ht49 T was most closely related to Actinomadura rhizosphaerae SDA37 T (98.8 %), Actinomadura logoneensis NEAU-G17 T (98.6 %), Actinomadura oligospora ATCC 43269 T (98.6 %) and Actinomadura gamaensis NEAU-Gz5 T (98.6 %). The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain NEAU-Ht49 T formed a cluster with A. rhizosphaerae SDA37 T , A. logoneensis NEAU-G17 T , A. oligospora ATCC 43269 T , A. gamaensis NEAU-Gz5 T and Actinomadura rupiterrae CS5-AC15 T (96.4 %). Meso-diaminopimelic acid was detected in its cell walls and glucose, madurose, mannose and ribose were detected in whole-cell hydrolysate. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside and two unidentified lipids. The majoy menaquinone was MK-10(H 6 ) and the minor menaquinones were MK-9(H 4 ) and MK-9(H 8 ). The major fatty acids were C 16 : 0 , C 18 : 1 ω9 c , 10-methyl C 18 : 0 and iso-C 16 : 0 . Moreover, morphological and chemotaxonomic characteristics of properties of strain NEAU-Ht49 T also confirmed the affiliation of the isolate to the genus Actinomadura . However, DNA–DNA relatedness, physiological and biochemical data showed that strain NEAU-Ht49 T could be distinguished from its closest relatives. Therefore, strain NEAU-Ht49 T represents a novel species of the genus Actinomadura , for which the name Actinomadura harenae sp. nov. is proposed, with strain NEAU-Ht49 T (=CGMCC 4.7499 T =JCM 32659 T ) as the type strain.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.003819
Language:
English
Publisher:
Microbiology Society
Publication Date:
2020
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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