In:
New Phytologist, Wiley, Vol. 209, No. 3 ( 2016-02), p. 1106-1119
Abstract:
Viral infections cause plant chlorosis, stunting, necrosis or other symptoms. The down‐regulation of chloroplast‐related genes (Ch RG s) is assumed to be responsible for chlorosis. We identified the differentially expressed genes ( DEG s) in Rice stripe virus ( RSV )‐infected Nicotiana benthamiana , and examined the contribution of 75 down‐regulated DEG s to RSV symptoms by silencing them one by one using Tobacco rattle virus ( TRV )‐induced gene silencing. Silencing of 11 of the 75 down‐regulated DEG s caused plant chlorosis, and nine of the 11 were Ch RG s. Silencing of a down‐regulated DEG encoding the eukaryotic translation initiation factor 4A ( eIF 4A) caused leaf‐twisting and stunting that were visible on RSV ‐infected N. benthamiana . A region of RSV RNA 4 was complementary to part of eIF 4A mRNA and virus‐derived small interfering (vsi RNA s) from that region were present in infected N. benthamiana . When expressed as artificial micro RNA s, those vsi RNA s could target Nbe IF 4A mRNA for regulation. We provide experimental evidence supporting the association of Ch RG s with chlorosis and show that eIF 4A is involved in RSV symptom development. This is also the first report demonstrating that si RNA derived directly from a plant virus can target a host gene for regulation.
Type of Medium:
Online Resource
ISSN:
0028-646X
,
1469-8137
DOI:
10.1111/nph.2016.209.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
208885-X
detail.hit.zdb_id:
1472194-6
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