In:
Chemistry & Biodiversity, Wiley, Vol. 20, No. 2 ( 2023-02)
Abstract:
Panax vietnamensis is a valuable medicinal resource with promising preclinical applications. Ginsenosides, which are triterpenoids, are the primary active components in P. vietnamensis . Oxidosqualene cyclases (OSCs) catalyze the formation of the basic skeleton of triterpenes from 2,3‐oxidosqualene, which is a crucial step in the biosynthesis of triterpenoids. The OSCs involved in triterpenoid biosynthesis in P. vietnamensis have not yet been characterized. Four OSC genes ( PvOSC 1–4) were cloned from P. vietnamensis and functionally characterized via heterologous expression in yeast. Transgenic yeast expressing PvOSC 1, Pv OSC 3, and Pv OSC 4 produced the corresponding products β‐amyrin, cycloartenol, and dammarenediol‐II, respectively. PvOSC1, PvOSC3, and PvOSC4 are monofunctional OSCs. In this study, we characterized three PvOSC genes, providing a better understanding of the biosynthesis of triterpenoids in P. vietnamensis and the multiple choices of plant OSCs for metabolic engineering in yeast and other hosts.
Type of Medium:
Online Resource
ISSN:
1612-1872
,
1612-1880
DOI:
10.1002/cbdv.202200874
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
2139001-0
SSG:
12
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