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  • Ma, Kyung-Ho  (2)
  • Raveendar, Sebastin  (2)
  • Biodiversity Research  (2)
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  • Biodiversity Research  (2)
  • 1
    Online Resource
    Online Resource
    Comparative efficacy of four candidate DNA barcode regions for identification of Vicia species
    Cambridge University Press (CUP) ; 2017
    In:  Plant Genetic Resources Vol. 15, No. 4 ( 2017-08), p. 286-295
    Details
    In: Plant Genetic Resources, Cambridge University Press (CUP), Vol. 15, No. 4 ( 2017-08), p. 286-295
    Abstract: The genus Vicia L., one of the earliest domesticated plant genera, is a member of the legume tribe Fabeae of the subfamily Papilionoideae ( Fabaceae ). The taxonomic history of this genus is extensive and controversial, which has hindered the development of taxonomic procedures and made it difficult to identify and share these economically important crop resources. Species identification through DNA barcoding is a valuable taxonomic classification tool. In this study, four DNA barcodes (ITS2, matK , rbcL and psbA-trnH ) were evaluated on 110 samples that represented 34 taxonomically best-known species in the Vicia genus. Topologies of the phylogenetic trees based on an individual locus were similar. Individual locus-based analyses could not discriminate closely related Vicia species. We proposed a concatenated data approach to increase the resolving power of ITS2. The DNA barcodes matK , psbA-trnH and rbcL were used as an additional tool for phylogenetic analysis. Among the four barcodes, three-barcode combinations that included psbA-trnH with any two of the other barcodes (ITS2, matK or rbcL ) provided the best discrimination among Vicia species. Species discrimination was assessed with bootstrap values and considered successful only when all the conspecific individuals formed a single clade. Through sequencing of these barcodes from additional Vicia accessions, 17 of the 34 known Vicia species could be identified with varying levels of confidence. From our analyses, the combined barcoding markers are useful in the early diagnosis of targeted Vicia species and can provide essential baseline data for conservation strategies, as well as guidance in assembling germplasm collections.
    Type of Medium: Online Resource
    ISSN: 1479-2621 , 1479-263X
    URL: Article
    DOI: 10.1017/S1479262115000623
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2017
    detail.hit.zdb_id: 2180556-8
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Comparison of flavonoid contents and antioxidant activities of Vicia species
    Cambridge University Press (CUP) ; 2017
    In:  Plant Genetic Resources Vol. 15, No. 2 ( 2017-04), p. 119-126
    Details
    In: Plant Genetic Resources, Cambridge University Press (CUP), Vol. 15, No. 2 ( 2017-04), p. 119-126
    Abstract: A total of 27 accessions from ten Vicia species were investigated for flavonoid contents, total polyphenol contents, and DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2′-azinobis(3-ethylbenzothiazoline 6-sulfonic acid)] free radical-scavenging activities. The results revealed that NAC17 ( V. monantha ) and NAC14 ( V. hyrcanica ) had the highest total flavonoid content (1.42 ± 0.09 mg/g) and total polyphenol content [124.2 ± 0.5 μg/gallic acid equivalents (GAE) mg], respectively. Among four flavonoids, naringenin was detected at high concentrations in Vicia species. The DPPH and ABTS assays showed values in the range of 57.2 (IC 50 ) (NAC13, V. faba ) to 6530.0 (IC 50 ) (NAC24, V. sativa subsp . nigra ) and 19.1 μg/Trolox mg (NAC7, V. cracca ) to 253.4 μg/Trolox mg (NAC13, V. faba ), respectively. Among ten Vicia species, V. monantha and V. hyrcanica had the highest flavonoid content (1.31 ± 0.09 mg/g) and total polyphenol content (116.5 ± 2.0 μg/GAE mg), respectively. The highest antioxidant activity was detected in V. faba . These results will expand the flavonoid database and provide valuable information on Vicia species for the development of functional foods or feed-additive resources.
    Type of Medium: Online Resource
    ISSN: 1479-2621 , 1479-263X
    URL: Article
    DOI: 10.1017/S147926211500043X
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2017
    detail.hit.zdb_id: 2180556-8
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
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