In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 60, No. 11 ( 2010-11-01), p. 2613-2617
Abstract:
Two halophilic archaea, strains RO1-4 T and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4 T and RO1-64 were able to grow at 25–55 °C (optimum 40–42 °C), at 2.1–5.1 M NaCl (optimum 3.9 M NaCl), at 0.05–0.7 M MgCl 2 (optimum 0.3 M MgCl 2 ) and at pH 6.0–8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12 % (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4 T and RO1-64 were closely related to Halogeometricum borinquense PR3 T (98.0 and 98.2 % similarity, respectively) and Halosarcina pallida BZ256 T (97.8 and 97.9 %). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4 T and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA–DNA hybridization value between strains RO1-4 T and RO1-64 was 83.0 % and both strains showed low DNA–DNA relatedness with Halogeometricum borinquense PR3 T (42.5 and 50.1 % relatedness, respectively) and Halosarcina pallida BZ256 T (37.6 and 42.1 % relatedness). It was concluded that strains RO1-4 T and RO1-64 represent a novel species of the genus Halogeometricum , for which the name Halogeometricum rufum sp. nov. is proposed. The type strain is RO1-4 T (=CGMCC 1.7736 T =JCM 15770 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.019463-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2010
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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