In:
Epigenomics, Future Medicine Ltd, Vol. 13, No. 10 ( 2021-05), p. 793-808
Abstract:
Aim: To reveal transcriptome-wide N6-methyladenosine (m 6 A) methylome of coronary artery disease (CAD). Materials & methods: The m 6 A levels of RNA from peripheral blood mononuclear cells measured by colorimetry were significantly decreased in CAD cases. Transcriptome-wide m 6 A methylome profiled by methylated RNA immunoprecipitation sequencing (MeRIP-seq) identified differentially methylated m 6 A sites within both mRNAs and lncRNAs between CAD and control group. Results: Bioinformatic analysis indicated that differentially methylated genes were involved in the pathogenesis of atherosclerosis. MeRIP-quantitative real-time PCR assay confirmed the reliability of MeRIP-seq data. Finally, the rat carotid artery balloon injury model was performed to confirm the role of m 6 A demethylase FTO in neointima formation. Conclusion: Our study provided a resource of differentially methylated m 6 A profile for uncovering m 6 A biological functions in the pathogenesis of CAD.
Type of Medium:
Online Resource
ISSN:
1750-1911
,
1750-192X
DOI:
10.2217/epi-2020-0372
Language:
English
Publisher:
Future Medicine Ltd
Publication Date:
2021
SSG:
12
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