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  • Grossart, Hans-Peter  (3)
  • Biodiversitätsforschung  (3)
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  • Biodiversitätsforschung  (3)
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  • 1
    Online-Ressource
    Online-Ressource
    Microbiology Society ; 2012
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 62, No. 1 ( 2012-01-01), p. 124-128
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 62, No. 1 ( 2012-01-01), p. 124-128
    Kurzfassung: A Gram-negative, motile, rod-shaped bacterial strain, HP15 T , was isolated from aggregates taken from surface waters of the German Wadden Sea (German Bight). Of 82 marine isolates, HP15 T was chosen for further study because of its high potential to induce production of transparent exopolymeric particles and aggregate formation while interacting with the diatom Thalassiosira weissflogii . HP15 T grew optimally at 34–38 °C and pH 7.0–8.5, and was able to tolerate salt concentrations of 0.5–20 % (w/v) NaCl. HP15 T was characterized chemotaxonomically by possessing ubiquinone-9 as the major respiratory lipoquinone, as well as C 16 : 0 , C 18 : 1 ω9 c and C 16 : 1 ω7 c /iso-C 15 : 0 2-OH as the predominant fatty acids. The DNA G+C content of strain HP15 T was 56.9 mol%. The closest relative based on 16S rRNA gene sequence analysis was the type strain of Marinobacter flavimaris , with 99 % similarity. Whole-genome relatedness values of HP15 T to the type strains of M. flavimaris , Marinobacter salsuginis , Marinobacter lipolyticus and Marinobacter algicola were less than 70 %, as determined by DNA–DNA hybridization. On the basis of phenotypic and chemotaxonomic properties as well as phylogenetic analyses, the isolate represents a novel species, Marinobacter adhaerens sp. nov.; the type strain is HP15 T ( = DSM 23420 T  = CIP 110141 T ).
    Materialart: Online-Ressource
    ISSN: 1466-5026 , 1466-5034
    Sprache: Englisch
    Verlag: Microbiology Society
    Publikationsdatum: 2012
    ZDB Id: 215062-1
    ZDB Id: 2056611-6
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Online-Ressource
    Online-Ressource
    American Society for Microbiology ; 2012
    In:  Applied and Environmental Microbiology Vol. 78, No. 19 ( 2012-10), p. 6900-6907
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 78, No. 19 ( 2012-10), p. 6900-6907
    Kurzfassung: Alga-bacterium interactions are crucial for aggregate formation and carbon cycling in aquatic systems. To understand the initiation of these interactions, we investigated bacterial chemotaxis within a bilateral model system. Marinobacter adhaerens HP15 has been demonstrated to attach to the diatom Thalassiosira weissflogii and induce transparent exopolymeric particle and aggregate formation. M. adhaerens possesses one polar flagellum and is highly motile. Bacterial cells were attracted to diatom cells, as demonstrated by addition of diatom cell homogenate or diatom culture supernatant to soft agar, suggesting that chemotaxis might be important for the interaction of M. adhaerens with diatoms. Three distinct chemotaxis-associated gene clusters were identified in the genome sequence of M. adhaerens , with the clusters showing significant sequence similarities to those of Pseudomonas aeruginosa PAO1. Mutations in the genes cheA , cheB , chpA , and chpB , which encode histidine kinases and methylesterases and which are putatively involved in either flagellum-associated chemotaxis or pilus-mediated twitching motility, were generated and mutants with the mutations were phenotypically analyzed. Δ cheA and Δ cheB mutants were found to be swimming deficient, and all four mutants were impaired in biofilm formation on abiotic surfaces. Comparison of the HP15 wild type and its chemotaxis mutants in cocultures with the diatom revealed that the fraction of bacteria attaching to the diatom decreased significantly for mutants in comparison to that for the wild type. Our results highlight the importance of M. adhaerens chemotaxis in initiation of its interaction with the diatom. In-depth knowledge of these basic processes in interspecies interactions is pivotal to obtain a systematic understanding of organic matter flux and nutrient cycling in marine ecosystems.
    Materialart: Online-Ressource
    ISSN: 0099-2240 , 1098-5336
    RVK:
    Sprache: Englisch
    Verlag: American Society for Microbiology
    Publikationsdatum: 2012
    ZDB Id: 223011-2
    ZDB Id: 1478346-0
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    In: Journal of Microbiological Methods, Elsevier BV, Vol. 87, No. 2 ( 2011-11), p. 176-183
    Materialart: Online-Ressource
    ISSN: 0167-7012
    Sprache: Englisch
    Verlag: Elsevier BV
    Publikationsdatum: 2011
    ZDB Id: 1483012-7
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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