In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 74, No. 19_Supplement ( 2014-10-01), p. 516-516
Abstract:
Introduction: Most satellite repeats in pericentmeric heterochromatin are epigenetically silenced and rarely transcribed into RNAs. However, in some pathological conditions, satellite RNA are transcribed and may deregulate cellular homeostasis. Recently, human satellite repeat II (HSATII) RNA were reported to be highly and specifically detected in human pancreatic cancers. However, precise localization of HSATII RNA expression in pancreatic cancer tissues and the time-course of its expression during carcinogenesis are unknown, mainly due to the technical difficulties to detect repetitive RNA sequences, which are difficult to amplify by PCR-based method. In this study, we determined the precise localization of HSATII RNA expression in pancreatic carcinoma tissues and developed a new method to detect HSATII RNA from the patient serum. Methods: 1) Highly sensitive and specific custom-made locked nucleic acid-modified oligonucleotide probes were synthesized to detect HSATII RNA expression by Northern blotting and RNA in situ hybridization. Resected tissues containing pancreatic carcinoma lesions and those adjacent lesions in the same section from individual patients were used to determine the precise localization of HSATII RNA expression, along with tissue arrays. 2) Total RNAs were extracted from the serum of pancreatic cancer, intraductal papillary mucinous neoplasm (IPMN), and non-cancerous patients. Custom-made biotinylated RNA probes for HSATII RNA were hybridized with the patient RNA. Paired RNA was collected, and visualized by applying modified RNA protection method. Results: 1) While no HSATII RNA expression was detected in pancreatic tissues without carcinoma, it was expressed in almost all pancreatic carcinoma tissues. Interestingly, the expression levels were clearly higher in non-cancerous lesions adjacent to carcinoma rather than in cancerous lesions themselves. 2) HSATII RNA was detected from 200 µl of the serum of pancreatic cancer and IPMN patients, whereas not detected from non-cancer patients. Conclusion: HSATII RNA is highly expressed in the tissues surrounding human pancreatic cancerous lesions. This non-coding RNA could be detected in the serum of pancreatic cancer patients as well as patients with pre-cancerous lesions. These results may facilitate the development of novel methods to determine high-risk patients of pancreatic cancer. Citation Format: Takahiro Kishikawa, Motoyuki Otsuka, Takeshi Yoshikawa, Motoko Ohno, Kazuhiko Koike. Detection of human satellite RNA in the serum of high-risk patients of pancreatic cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 516. doi:10.1158/1538-7445.AM2014-516
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2014-516
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2014
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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