In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 7 ( 2022-7-18), p. e1010308-
Abstract:
The conserved shelterin complex caps chromosome ends to protect telomeres and regulate telomere replication. In fission yeast Schizosaccharomyces pombe , shelterin consists of telomeric single- and double-stranded DNA-binding modules Pot1-Tpz1 and Taz1-Rap1 connected by Poz1, and a specific component Ccq1. While individual structures of the two DNA-binding OB folds of Pot1 (Pot1 OB1 -GGTTAC and Pot1 OB2 -GGTTACGGT) are available, structural insight into recognition of telomeric repeats with spacers by the complete DNA-binding domain (Pot1 DBD ) remains an open question. Moreover, structural information about the Tpz1-Ccq1 interaction requires to be revealed for understanding how the specific component Ccq1 of S . pombe shelterin is recruited to telomeres to function as an interacting hub. Here, we report the crystal structures of Pot1 DBD -single-stranded-DNA, Pot1 372-555 -Tpz1 185-212 and Tpz1 425-470 -Ccq1 123-439 complexes and propose an integrated model depicting the assembly mechanism of the shelterin complex at telomeres. The structure of Pot1 DBD -DNA unveils how Pot1 recognizes S . pombe degenerate telomeric sequences. Our analyses of Tpz1-Ccq1 reveal structural basis for the essential role of the Tpz1-Ccq1 interaction in telomere recruitment of Ccq1 that is required for telomere maintenance and telomeric heterochromatin formation. Overall, our findings provide valuable structural information regarding interactions within fission yeast shelterin complex at 3’ ss telomeric overhang.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010308
DOI:
10.1371/journal.pgen.1010308.g001
DOI:
10.1371/journal.pgen.1010308.g002
DOI:
10.1371/journal.pgen.1010308.g003
DOI:
10.1371/journal.pgen.1010308.g004
DOI:
10.1371/journal.pgen.1010308.g005
DOI:
10.1371/journal.pgen.1010308.s001
DOI:
10.1371/journal.pgen.1010308.s002
DOI:
10.1371/journal.pgen.1010308.s003
DOI:
10.1371/journal.pgen.1010308.s004
DOI:
10.1371/journal.pgen.1010308.s005
DOI:
10.1371/journal.pgen.1010308.s006
DOI:
10.1371/journal.pgen.1010308.s007
DOI:
10.1371/journal.pgen.1010308.s008
DOI:
10.1371/journal.pgen.1010308.s009
DOI:
10.1371/journal.pgen.1010308.s010
DOI:
10.1371/journal.pgen.1010308.s011
DOI:
10.1371/journal.pgen.1010308.s012
DOI:
10.1371/journal.pgen.1010308.s013
DOI:
10.1371/journal.pgen.1010308.s014
DOI:
10.1371/journal.pgen.1010308.s015
DOI:
10.1371/journal.pgen.1010308.r001
DOI:
10.1371/journal.pgen.1010308.r002
DOI:
10.1371/journal.pgen.1010308.r003
DOI:
10.1371/journal.pgen.1010308.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
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