In:
GCB Bioenergy, Wiley, Vol. 9, No. 1 ( 2017-01), p. 262-269
Abstract:
In this study, we used ELISA for quantification of PPDK in photosynthesizing leaves of miscanthus. We cloned a fragment of the gene encoding PPDK , purified the resulting protein by affinity chromatography, identified it using MALDI mass spectrometry, and obtained monoclonal antibodies by immunizing BALB /c mice. Selectivity of monoclonal antibodies was assessed by Western blot using the protein extracts of Soranovskii. The presence of PPDK was again verified by MALDI mass spectrometry. Therefore, we developed and tested the method for determining PPDK quantity in miscanthus using ELISA .
Type of Medium:
Online Resource
ISSN:
1757-1693
,
1757-1707
DOI:
10.1111/gcbb.2017.9.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2495051-8
SSG:
12
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