In:
Nematology, Brill, Vol. 6, No. 2 ( 2004), p. 279-285
Abstract:
Pine wood wilt disease caused by the pine wood nematode, Bursaphelenchus
xylophilus , has been a serious problem in the southern regions of Korea. Efficient diagnosis of B. xylophilus from infected pine wood specimens is
critical for the management of this pest. Traditional microscopic examination often results in an erroneous identification because a closely
related non-pathogenic species, B. mucronatus, has a great degree of morphological similarity to B. xylophilus. In an attempt to search for
reliable molecular markers for the discrimination of these species, we have cloned the 5S rRNA genomic DNA fragments containing both coding and
intergenic spacer (IGS) regions from B. xylophilus and B. mucronatus through a homology-probing PCR strategy. Sequence analyses revealed that coding
sequences of the 5S rRNA gene from the two species are almost identical (98.3% homology) but that the IGS sequences differ substantially between the
species. Based on the IGS sequence differences (69.7% homology), we designed species-specific primer sets and developed a PCR-based diagnosis protocol
for the identification and discrimination of the two nematode species on a molecular basis.
Type of Medium:
Online Resource
ISSN:
1388-5545
,
1568-5411
DOI:
10.1163/1568541041217915
Language:
Unknown
Publisher:
Brill
Publication Date:
2004
detail.hit.zdb_id:
2019332-4
SSG:
12
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