In:
International Journal of Oral Science, Springer Science and Business Media LLC, Vol. 10, No. 2 ( 2018-04-23)
Abstract:
β-Catenin plays a critical role in cartilage formation and development. To further understand the role of β-catenin in osteoarthritis (OA) development in temporomandibular joint (TMJ), we have generated β-catenin conditional activation mice ( β-cat ( ex3 ) Agc1CreER ) by breeding Agc1-CreER mice with β-catenin flox ( ex3 )/ + mice. Results of histologic analysis showed the progressive TMJ defects in 3- and 6-month-old β-cat ( ex3 ) Agc1CreER mice (tamoxifen induction was performed at 2 weeks of age), including decreased chondrocyte numbers in the superficial layer associated with less Alcian blue staining, increased numbers of hypertrophic chondrocytes in deep layers, and rough articular surface. Compared to the TMJ phenotype of β-cat ( ex3 ) Col2CreER mice, β-cat ( ex3 ) Agc1CreER mice showed much severe morphological defects in the superficial layer of TMJ. This may reflect that Agc1-CreER mice could efficiently target cells in the superficial layer of TMJ. Results of immunostaining showed significantly increased expression of MMP13, Col-X, Adamts4, and Adamts5 in TMJ of β-cat ( ex3 ) Agc1CreER mice. Results of proliferating cell nuclear antigen (PCNA), Ki67, and terminal deoxinucleotidyl transferase-mediated dUTP-fluorescein nick end labeling (TUNEL) staining further demonstrated that cell proliferation was decreased and cell apoptosis was increased in condylar cartilage of β-cat ( ex3 ) Agc1CreER mice. Our findings indicate that abnormal upregulation of β-catenin in TMJ leads to defects assembling to OA-like phenotype, further demonstrating that β-catenin plays a critical role in TMJ pathogenesis.
Type of Medium:
Online Resource
ISSN:
2049-3169
DOI:
10.1038/s41368-018-0016-z
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2018
detail.hit.zdb_id:
2569849-7
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