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  • OceanRep  (3)
  • OceanRep: Article in a Scientific Journal - peer-reviewed  (3)
  • American Chemical Society  (2)
  • American Society of Agronomy  (1)
  • 1
    Publication Date: 2017-05-30
    Description: High-performance liquid chromatography-electrospray ionization mass spectrometry was applied to the detection of the iron(III) complexes of the hydroxamate siderophores rhodotoluric acid, deferrioxamine B, and deferrichrome. Separation of the iron(III) complexes was obtained using a polystyrene-divinylbenzene stationary phase. The retention and responses of ferrioxamine and ferrichrome were optimal when a gradient elution program with methanol and 0.1% (v/v) formic acid as the mobile phases was used. These conditions were also suitable for the retention and separation of the uncomplexed ligands. Retention of iron(III) rhodotoluate was improved when formic acid was replaced by the ion-pairing reagent heptafluorobutyric acid (0.1%). Detection limits for the ferric complexes, defined as 3 SD of the lowest determined standard, were 26 nM for iron(III) rhodotoluate, 0.23 nM for ferrioxamine, and 0.40 nM for ferrichrome. A protocol for the solid-phase extraction of these hydroxamate siderophores from seawater was developed and applied to the extraction of siderophores from enriched incubated seawater samples.
    Type: Article , PeerReviewed
    Format: text
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  • 2
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    American Society of Agronomy
    In:  Journal of Environment Quality, 30 (5). pp. 1738-1746.
    Publication Date: 2020-07-24
    Description: This paper describes the results of an export coefficient modeling approach to predict total phosphorus (TP) loading in the Frome catchment, Dorset, UK from point and diffuse sources on a seasonal (monthly) basis in 1998 and on an annual basis for 1990–1998. The model predicted an annual TP load of 25605 kg yr−1, compared with an observed (measured) value of 23400 kg yr−1 Monthly loads calculated using the export coefficient model agreed well with monthly observed values except in months of variable discharge, when observed values were low, probably due to infrequent, and therefore unrepresentative, sampling. Comparison between filterable reactive phosphorus (FRP) and TP concentrations observed in the period 1990–1997 showed that trends in FRP could be estimated from trends in TP. A sensitivity analysis (varying individual export coefficients by ±10%) showed that sewage treatment works (STWs) (3.5%), tilled land (2.7%), meadow–verge–seminatural (1.0%), and mown and grazed turf (0.6%) had the most significant effect (percent difference from base contribution) on model prediction. The model was also used to estimate the effect of phosphorus stripping at STWs in order to comply with a pending change in the European Union wastewater directive. Theoretical reduction of TP from the largest STW in the catchment gave a predicted reduction in TP loading of 2174 kg yr−1 This illustrates the value of this seasonal export coefficient model as a practical management tool.
    Type: Article , PeerReviewed
    Format: text
    Location Call Number Limitation Availability
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  • 3
    Publication Date: 2017-05-30
    Description: High-performance liquid chromatography-electrospray ionization mass spectrometry was applied to the detection of the iron(III) complexes of the hydroxamate siderophores rhodotoluric acid, deferrioxamine B, and deferrichrome. Separation of the iron(III) complexes was obtained using a polystyrene-divinylbenzene stationary phase. The retention and responses of ferrioxamine and ferrichrome were optimal when a gradient elution program with methanol and 0.1% (v/v) formic acid as the mobile phases was used. These conditions were also suitable for the retention and separation of the uncomplexed ligands. Retention of iron(III) rhodotoluate was improved when formic acid was replaced by the ion-pairing reagent heptafluorobutyric acid (0.1%). Detection limits for the ferric complexes, defined as 3 SD of the lowest determined standard, were 26 nM for iron(III) rhodotoluate, 0.23 nM for ferrioxamine, and 0.40 nM for ferrichrome. A protocol for the solid-phase extraction of these hydroxamate siderophores from seawater was developed and applied to the extraction of siderophores from enriched incubated seawater samples.
    Type: Article , PeerReviewed
    Format: text
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
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