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  • OceanRep  (2)
  • OceanRep: Article in a Scientific Journal - peer-reviewed  (2)
  • 2015-2019  (2)
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  • OceanRep  (2)
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  • OceanRep: Article in a Scientific Journal - peer-reviewed  (2)
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  • 1
    Publication Date: 2021-01-08
    Description: In recent years, there has been a widespread deployment of submersible fluorescence sensors by water utilities. They are used to measure diagnostic pigments and estimate algae and cyanobacteria abundance in near real-time. Despite being useful and promising tools, operators and decision-makers often rely on the data provided by these probes without a full understanding of their limitations. As a result, this may lead to wrong and misleading estimations which, in turn, means that researchers and technicians distrust these sensors. In this review paper, we list and discuss the main limitations of such probes, as well as identifying the effect of environmental factors on pigment production, and in turn, the conversion to cyanobacteria abundance estimation. We argue that a comprehensive calibration approach to obtain reliable readings goes well beyond manufacturers’ recommendations, and should involve several context-specific experiments. We also believe that if such a comprehensive set of experiments is conducted, the data collected from fluorescence sensors could be used in artificial intelligence modelling approaches to reliably predict, in near real-time, the presence and abundance of different cyanobacteria species. This would have significant benefits for both drinking and recreational water management, given that cyanobacterial toxicity, and taste and odour compounds production, are species-dependent.
    Type: Article , PeerReviewed
    Format: text
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  • 2
    Publication Date: 2022-01-31
    Description: Previous studies have shown that under laboratory conditions, dissolved organic matter (DOM) leached from plants can be differentially more phytotoxic to cyanobacteria, compared to green algae. This study examined how DOM source and transformation processes (microbial and photochemical) affect its chemical composition and phytotoxicity towards a cultured species of cyanobacteria (Raphidiopsis raciborskii) using a factorial experimental design. To complement cyanobacterial bioassays, the chemical composition and associated changes in DOM were determined using spectroscopic (nuclear magnetic resonance (NMR) and absorbance) and elemental analyses. Sunlight exposed DOM from leaves of the terrestrial plants, Casuarina cunninghamiana and Eucalyptus tereticornis had the most phytotoxic effect compared to DOM not exposed to sunlight. This phytotoxic DOM was characterised by relatively low nitrogen content, containing highly coloured and relatively high molecular mass constituents. Both mixed effect model and PCA approaches to predict inhibition of photosynthetic yield indicated phytotoxicity could be predicted (P 〈 0.001) based upon the following parameters: C: N ratio; gilvin, and lignin-derived phenol content of DOM. Parallel proton-detected 1D and 2D NMR techniques showed that glucose anomers were the major constituents of fresh leachate. With ageing, glucose anomers disappeared and products of microbial transformation appeared, but there was no indication of the appearance of additional phytotoxic compounds. This suggests that reactive oxygen species may be responsible, at least partially, for DOM phytotoxicity. This study provides important new information highlighting the characteristics of DOM that link with phytotoxic effects.
    Type: Article , PeerReviewed
    Format: text
    Location Call Number Limitation Availability
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