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Microextraction of Nuclear Proteins from Single Maize Embryos (1997)

Busk, Peter K. ; Pagès, Montserrat

Springer

Plant molecular biology reporter 15 (1997), S. 371-376

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ISSN: 1572-9818

Keywords: band shift assay ; DNA binding protein ; maize ; nuclear protein extraction

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The analysis of DNA binding proteins can be difficult when only small quantities of tissue expressing the desired protein are available. We present a protocol for the preparation of nuclear extracts from as little as 100 mg of tissue. This protocol is well suited for extraction of DNA binding proteins from tissues that are difficult to obtain in large quantities such as maize embryos.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007428802474

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