ISSN:
1573-4978
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Modification of 40S ribosomal subunits from Saccharomyces cerevisiae with dimethylmaleic anhydride (DMMA Abbreviation: DMMA, 2,3-dimethylmaleic anhydride. ), a reagent for protein amino groups, is accompanied by loss of polypeptide-synthesizing activity and by dissociation of proteins from the particles. The protein-deficient ribosomal particles, originated from 40S subunits by treatment with dimethylmaleic anhydride at a molar ratio of reagent to particle of 250, can partially reconstitute active subunits upon addition of the corresponding released proteins, and regeneration of the modified amino groups.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00419733
