ISSN:
1573-5176
Keywords:
picophytoplankton
;
C-phycocyanin
;
C-phycoerythrin
;
R-phycoerythrin
;
fluorescence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A sensitive and specific technique is described for the estimation of phycobiliprotein in freshwater and marine picophytoplankton. The method uses fluorescent properties to detect phycoerythrin concentrations as low as 40 ng L-1 from a 1 L water sample and is capable of distinguishing between R-phycoerythrin, C-phycocyanin and C-phycoerythrin. The application of the method to the study of natural picophytoplankton populations in marine and freshwater environments is described. Nitrate concentrations appear to influence picophytoplankton cellular C-phycoerythrin concentrations in surface waters and increasing cellular C-phycoerythrin fluorescence with water depth suggests that this pigment plays a role as a photosynthetic accessory pigment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008085719486