Description:    Nitrous oxide (N 2 O) emissions from grazed grasslands are estimated to be approximately 28% of global anthropogenic N 2 O emissions. Estimating the N 2 O flux from grassland soils is difficult because of its episodic nature. This study aimed to quantify the N 2 O emissions, the annual N 2 O flux and the emission factor (EF), and also to investigate the influence of environmental and soil variables controlling N 2 O emissions from grazed grassland. Nitrous oxide emissions were measured using static chambers at eight different grasslands in the South of Ireland from September 2007 to August 2009. The instantaneous N 2 O flux values ranged from -186 to 885.6 μg N 2 O-N m −2  h −1 and the annual sum ranged from 2 ± 3.51 to 12.55 ± 2.83 kg N 2 O-N ha −1  y −1 for managed sites. The emission factor ranged from 1.3 to 3.4%. The overall EF of 1.81% is about 69% higher than the Intergovernmental Panel on Climate Change (IPCC) default EF value of 1.25% which is currently used by the Irish Environmental Protection Agency (EPA) to estimate N 2 O emission in Ireland. At an N applied of approximately 300 kg ha −1  y −1 , the N 2 O emissions are approximately 5.0 kg N 2 O-N ha −1 y −1 , whereas the N 2 O emissions double to approximately 10 kg N ha −1 for an N applied of 400 kg N ha −1  y −1 . The sites with higher fluxes were associated with intensive N-input and frequent cattle grazing. The N 2 O flux at 17°C was five times greater than that at 5°C. Similarly, the N 2 O emissions increased with increasing water filled pore space (WFPS) with maximum N 2 O emissions occurring at 60–80% WFPS. We conclude that N application below 300 kg ha −1  y −1 and restricted grazing on seasonally wet soils will reduce N 2 O emissions. Content Type Journal Article Pages 1-20 DOI 10.1007/s10021-011-9434-x Authors Rashad Rafique, Department of Civil and Environmental Engineering, Centre for Hydrology, Micrometeorology and Climate Change, University College Cork, Cork, Ireland Deirdre Hennessy, Department of Animals &, Grassland Science Research, Teagasc-Moorpark, Fermoy, Ireland Gerard Kiely, Department of Civil and Environmental Engineering, Centre for Hydrology, Micrometeorology and Climate Change, University College Cork, Cork, Ireland Journal Ecosystems Online ISSN 1435-0629 Print ISSN 1432-9840