Description: Publication date: 15 January 2015 Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 418 Author(s): M. Lamentowicz , M. Gałka , Ł. Lamentowicz , M. Obremska , N. Kühl , A. Lücke , V.E.J. Jassey In this study, we present a record spanning the last 4000 years from a Baltic bog (Kusowskie Bagno) in northern Poland. Using numerous biotic and abiotic proxies, such as testate amoebae (depth to water table reconstructions), stable carbon isotopes ( 13 C), plant macrofossils (proxies for local vegetation and mire surface wetness), pollen and spores (proxies for regional vegetation and human impact), we reconstructed and identified the regional hydro-climatic signal of Kusowskie Bagno bog and compared it to other bog records around the Baltic Sea. Our aims were to: 1) combine the species traits of bryophytes and testate amoebae, and more common proxies (isotopes, plant micro-and macro-remains) to infer past peatland development, 2) compare the hydro-climatic signal of Kusowskie Bagno bog to existing records around the Baltic Sea. We found that Kusowskie Bagno bog was very wet during the last 4000 years, and even drainage and peat exploitation had not disturbed its hydrology in northern part in the last 200 years. Carbon isotopes and plant macrofossils were significantly related to specific traits of testate amoebae, which in turn reflected the water table changes over the last 4000 years. Kusowskie Bagno recorded at least the following wet shifts: AD 250, 550, 850, 1250 and 1700, while wet conditions occurred during the Migration period at ca AD 550. Furthermore, the testate amoeba-based quantitative wetness reconstruction in Kusowskie Bagno bog resembles the pattern observed in other sites around the Baltic, i.e., Estonia, Finland, Ireland, northern Britain and the 7500-year record from the Stążki bog, northern Poland. Our results provided statistically validated evidence that interactions between plant and microbe need to be more considered further to reconstruct past hydrological. This is the first study of past hydro-climatic changes in peatlands based upon a trait-based approach.