In:
Skeletal Muscle, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2023-01-06)
Abstract:
The dystrophin-glycoprotein complex (DGC) is a critical adhesion complex of the muscle cell membrane, providing a mechanical link between the extracellular matrix (ECM) and the cortical cytoskeleton that stabilizes the sarcolemma during repeated muscle contractions. One integral component of the DGC is the transmembrane protein, sarcospan (SSPN). Overexpression of SSPN in the skeletal muscle of mdx mice (murine model of DMD) restores muscle fiber attachment to the ECM in part through an associated increase in utrophin and integrin adhesion complexes at the cell membrane, protecting the muscle from contraction-induced injury. In this study, we utilized transcriptomic and ECM protein-optimized proteomics data sets from wild-type, mdx , and mdx transgenic ( mdx TG ) skeletal muscle tissues to identify pathways and proteins driving the compensatory action of SSPN overexpression. Methods The tibialis anterior and quadriceps muscles were isolated from wild-type, mdx , and mdx TG mice and subjected to bulk RNA-Seq and global proteomics analysis using methods to enhance capture of ECM proteins. Data sets were further analyzed through the ingenuity pathway analysis (QIAGEN) and integrative gene set enrichment to identify candidate networks, signaling pathways, and upstream regulators. Results Through our multi-omics approach, we identified 3 classes of differentially expressed genes and proteins in mdx TG muscle, including those that were (1) unrestored (significantly different from wild type, but not from mdx ), (2) restored (significantly different from mdx , but not from wild type), and (3) compensatory (significantly different from both wild type and mdx ). We identified signaling pathways that may contribute to the rescue phenotype, most notably cytoskeleton and ECM organization pathways. ECM-optimized proteomics revealed an increased abundance of collagens II, V, and XI, along with β -spectrin in mdx TG samples. Using ingenuity pathway analysis, we identified upstream regulators that are computationally predicted to drive compensatory changes, revealing a possible mechanism of SSPN rescue through a rewiring of cell-ECM bidirectional communication. We found that SSPN overexpression results in upregulation of key signaling molecules associated with regulation of cytoskeleton organization and mechanotransduction, including Yap1, Sox9, Rho, RAC, and Wnt. Conclusions Our findings indicate that SSPN overexpression rescues dystrophin deficiency partially through mechanotransduction signaling cascades mediated through components of the ECM and the cortical cytoskeleton.
Type of Medium:
Online Resource
ISSN:
2044-5040
DOI:
10.1186/s13395-022-00311-x
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2023
detail.hit.zdb_id:
2595637-1
