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  • Electron microscopy  (597)
  • Life Sciences  (515)
  • 1975-1979  (1,112)
  • 1
    Electronic Resource
    Electronic Resource
    Time sequence and site of fluid accumulation in experimental neurogenic pulmonary edema (1976)
    Springer
    Research in experimental medicine 168 (1976), S. 219-227 
    Details
    ISSN: 1433-8580
    Keywords: Initial phase of neurogenic pulmonary edema ; Perfusion fixation ; HRP ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The initial phase of pulmonary edema development following intracranial pressure elevation was studied by means of transmission electron microscopy. Using perfusion fixation and application of a blood tracer (HRP horseradish peroxidase) the time sequence and site of fluid leakage out of pulmonary vessels was demonstrated: - passage of edema fluid through intercellular clefts of alveolar capillary endothelium - edema accumulation in alveolar interstitial tissue - draining of edema fluid from the alveolar septum to the interstitium of terminal bronchioli and to lymphatic vessels. An early interepithelial fluid leakage out of the alveolar wall remains questionable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851320
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  • 2
    Electronic Resource
    Electronic Resource
    Light and electron microscopic findings in five cases of cryoglobulinemic glomerulonephritis (1979)
    Springer
    Virchows Archiv 384 (1979), S. 29-44 
    Details
    ISSN: 1432-2307
    Keywords: Glomerulonephritis ; Cryoglobulinemia ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Renal tissue from five patients with cryoglobulinemia was studied by light and electron microscopy and immunofluorescence. None of the histologic features observed at the light microscopic level seems to be specific for cryoglobulinemia. Electron microscopic investigations have shown very large electron dense deposits in almost every examined lobule in all cases. The deposits displayed two main patterns; a homogeneous texture in two cases and tubular or annular structures in three cases. The patients with typically structured deposits had IgG-IgM cryoglobulinemia (2 cases) or monoclonal IgM cryoglobulinemia (1 case). The presence of IgM in cryoglobulinemia may be the cause of the peculiar structure of the deposits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427149
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  • 3
    Electronic Resource
    Electronic Resource
    Freeze fracture aspects of the spiral limbus (1978)
    Springer
    European archives of oto-rhino-laryngology and head & neck 221 (1978), S. 157-162 
    Details
    ISSN: 1434-4726
    Keywords: Gefrierbruchmethode ; Elektronenmikroskopie ; Zonulae occludentes ; Gap junctions ; Limbus spiralis ; Freeze fracture ; Electron microscopy ; Zonulae occludentes ; Gap junctions ; Spiral limbus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Replicas of the freeze fractured spiral limbus are studied in chinchillas. Zonulae occludentes are demonstrated between the interdental cells and the inner sulcus cells; gap junctions are described connecting interdental cells and inner sulcus cells to one another and they are found between the perilymphatic cells in the spiral limbus.
    Notes: Zusammenfassung Untersuchung des Limbus spiralis an Chinchillas mit Hilfe der Gefrierbruchmethode. Zonulae occludentes werden zwischen den Interdentalzellen und den Zellen des inneren Sulcus demonstriert; gap junctions verbinden die Interdentalzellen und die Zellen des inneren Sulcus miteinander, weiter finden sie sich zwischen den perilymphatischen Zellen des Limbus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01886290
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  • 4
    Electronic Resource
    Electronic Resource
    Adrenergic innervation of the eighth nerve and vestibular end organs in man (1979)
    Springer
    European archives of oto-rhino-laryngology and head & neck 224 (1979), S. 17-23 
    Details
    ISSN: 1434-4726
    Keywords: Eighth nerve ; Inner ear ; Human ; Fluorescence microscopy ; Electron microscopy ; Menière ; Neurilemoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fluorescence microscopy was used to examine the adrenergic, bloodvessel-independent innervation of the 8th nerve and inner ear specimens in patients with otoneurological diseases. Specifically fluorescent adrenergic axons were scantily seen among the myelinated nerve fibers in the 8th nerve and in the subepithelial regions of the end organs but not in the endolymphatic sac.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455219
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  • 5
    Electronic Resource
    Electronic Resource
    Metabolism and ultrastructure in ovaries of alloxan-diabetic juvenile rats (1976)
    Springer
    Archives of gynecology and obstetrics 220 (1976), S. 199-208 
    Details
    ISSN: 1432-0711
    Keywords: Rat ovaries ; Diabetes mellitus ; Alloxan ; ATP/ADP ; Lactate/pyruvate ; NADP/NADPH ; Glucose ; Protein content ; Hydrogen-coveying enzymes ; alkal. phosphatase ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wurde der Einfluß eines alloxan-induzierten Diabetes mellitus auf den Stoffwechsel und die Ultrastruktur juveniler Rattenovarien untersucht. Der Diabetes mellitus bewirkte folgende Änderungen imStoffwechsel: Abfall der Konzentration von ATP und NADPH, Anstieg des Lactat/Pyruvat-Quotienten über 40, Abfall des ATP/ADP-Quotienten unter 1, Aktivitätsminderung der wasserstoffübertragenden Enzyme G-6-DH, Isocitrat-DH, Malat-DH, Aktivitätszunahme der alkalischen Phosphatase, Erniedrigung des Proteingehaltes.Ultrastruktur: Nahezu vollständiges Verschwinden des rauhen endoplasmatischen Reticulums, Schrumpfung der Mitochondrien, Reduktion der cristae und Kondensation der Matrix. Das glatte endoplasmatische Reticulum bleibt unverändert, der Golgi-Komplex ist in seiner Ausdehnung reduziert. Leichte Extrahierbarkeit der Lipiddepots. Die Befunde sprechen für eine Hemmung der oxydativen Phosphorylierung sowie für eine Beeinträchtigung des Steroid- und Proteinstoffwechsels.
    Notes: Summary Tests were carried out on the influence of alloxan-induced diabetes mellitus on the metabolism and the ultrastructure of ovaries of juvenile rats. The diabetes mellitus caused the following changes in themetabolism: reduction in the concentration of ATP and NADPH, increase in the lactate/pyruvate quotient to above 40, reduction in the ATP/ADP quotient to below 1, reduction in the level of activity of the hydrogen-conveying enzymes G-6-P-dehydrogenase, isocitrate dehydrogenase and malate dehydrogenase, increase in the level of activity of the alkaline phosphatase, reduction of the protein content. Ultrastructure almost complete disappearance of the rough endoplasmic reticulum, shrinkage of the mitochondria, reduction of the cristae and condensation of the matrix. The smooth endoplasmic reticulum remains unchanged, the extent of the Golgi-complex is reduced. Easy removal of the lipid deposits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00667748
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  • 6
    Electronic Resource
    Electronic Resource
    Feinstrukturelle Untersuchungen über das Nuclear-Channel-System im menschlichen Endometrium (1977)
    Springer
    Archives of gynecology and obstetrics 223 (1977), S. 195-204 
    Details
    ISSN: 1432-0711
    Keywords: Electron microscopy ; Human endometrium ; Nucleus ; Nucleolus ; Channel system ; Elektronenmikroskopie ; Menschliches Endometrium ; Nukleus ; Nukleolus ; Channel-System
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Das Nuclear-Channel-System ist eine runde bis ellipsoide Struktur, deren Tubuli querangeschnitten oder längsangeschnitten in eine elektronendichte Matrix eingebettet sind. Das NCS liegt meist der Kernmembran dicht an, die in diesem Bereich Einziehungen aufweist. Gelegentlich ist ein Kontakt der Kernmembran mit der Membran eines Tubulus zu beobachten. Im NCS sind Partikel festzustellen, die den 150 Å großen Partikeln des Nucleolus vergleichbar sind, was auf einen Gehalt des NCS an RNA schließen läßt. In hier vorliegenden Untersuchungen konnte das NCS in Zellkernen endometrialer Drüsenzellen während der mittleren und späten Proliferationsphase beobachtet werden. Diese Befunde sprechen gegen eine zwingende Abhängigkeit des Auftretens des NCS vom Zeitpunkt der Ovulation. Das NCS kann somit nicht als eine für postovulatorisches Endometrium typische Struktur angesehen werden. Offensichtlich bestehen zwischen den hormonellen Faktoren und der Ausbildung des NCS Beziehungen, deren Bedeutung bis heute nicht sicher beurteilt werden kann.
    Notes: Summary The Nuclear Channel System (NCS) is a distinct endometrial pattern as observed by transmission electron microscopy. The NCS was identified in the present study in cell nuclei of endometrial glands during mid and late proliferation. These observations do not support the concept that the NCS develops at the ovulation, and is therefore not a typical post ovulatory structure. It is suggested that there are relations between hormonal factors and the development of the NCS which are as yet no clarified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00667389
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastrukturell-morphometrische Untersuchungen an Leberbiopsien — Der Einfluß von Ovulationshemmern auf die menschliche Leber (1977)
    Springer
    Archives of gynecology and obstetrics 223 (1977), S. 205-211 
    Details
    ISSN: 1432-0711
    Keywords: Electron microscopy ; Stereology ; Human liver ; Oral contraceptives ; Elektronenmikroskopie ; Stereologie ; Menschliche Leber ; Orale Kontrazeptiva
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wurden Leberbiopsien von 12 Frauen feinstrukturell-morphometrisch untersucht, die über einen Zeitraum von 2 Monaten bis zu 8 Jahren ein Kombinationspräparat zur hormonellen Antikonzeption eingenommen hatten. Die stereologischen Daten wurden bereits vorher erstellten Basiswerten für normale Lebern (Roessner et al., 1977) gegenübergestellt und auf signifikante Unterschiede hin überprüft (p〈0,001). Eine deutliche Volumenzunahme lysosomaler Zelleinschlüsse pro ml Hepatocyten und eine leichte Cytoplasmavergrößerung bei gleichzeitiger Verkleine-rung der Nuclei kennzeichnen die Veränderungen der Hepatocyten. Alle übrigen ermittelten Parameter lassen den Schluß zu, daß wesentliche Alterationen der Ultrastruktur der menschlichen Leber nach hormoneller Kontrazeption nicht auftreten. Ob damit auch funktionelle Alterationen auszuschließen sind, kann hier nicht beantwortet werden.
    Notes: Summary Liver biopsies from 12 healthy women were investigated morphometrically — according to the methods of Weibel (1969). All women had taken an oral contraceptive agent during 2 til 76 months. The evaluated stereological datas were compared with base-line datas for normal human liver biopsies, which were elaborated in a previous investigation (Roessner et al., 1977). Statistical analysis was performed on an IBM 360/50 computer system, including F- and student's t-test. In the liver biopsy-specimen from the women, which were on oral contraceptives, the volume-density of lysosomes per ml hepatocytes shows a pronounced, significant (p〈0.001) increase. A moderate increase of cytoplasm is combined with decrease of nuclei-volume. The surface-densities of the rough and smooth endoplasmatic reticulum do not differ significantly from the datas calculated from normal human liver biopsies. All other datas too demonstrate that there are no striking changes in the ultrastructure of human liver, caused by oral contraceptives. Functional alterations however can not be excluded.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00667390
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  • 8
    Electronic Resource
    Electronic Resource
    Studies on the development of cell contacts and of the intercellular matrix during decidualization in the rat (1979)
    Springer
    Archives of gynecology and obstetrics 227 (1979), S. 103-117 
    Details
    ISSN: 1432-0711
    Keywords: Decidua ; Interzelluläre Verbindungen ; Gefrierätzen ; Elektronenmikroskopie ; Fibrinoid ; Decidua ; Intercellular junctions ; Freeze-fracturing ; Electron microscopy ; Fibrinoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Cell contacts between developing decidual cells were examined in thin sections and in replicas of freeze-cleaved tissue of rat uterus. At the time of implantation only puncta adhaerentia were found between stromal cells. Gap junctions appeared 24 h after decidualization and thereafter increased in number and extent. Thus, there is little morphological evidence for a propagation of the initial stimulus for decidualization via gap junctions. An increase in amorphous, extracellular material is noted after decidualization with a concomitant decrease of collagen fibers. This amorphous material probably represents fibrinoid.
    Notes: Zusammenfassung Die Zellkontakte zwischen sich entwickelnden Deciduazellen wurden sowohl mit der Dünnschnittechnik als auch mit der Gefrierätzmethode im Rattenuterus untersucht. Zum Zeitpunkt der Implantation wurden nur Puncta adhaerentia zwischen Stromazellen gefunden. Nexus (gap junctions) erschienen 24 h nach Beginn der Decidualisierung und nahmen danach an Zahl und Größe zu. Diese morphologischen Befunde widersprechen der Ansicht, daß sich der initiale Reiz für die Decidualisierung der Stromazellen über Nexus ausbreitet. Nach der Decidualisierung wurde eine Zunahme von amorpher, extrazellulärer Substanz gleichzeitig mit einer Abnahme von Kollagenfasern gesehen. Diese amorphe Substanz stellt wahrscheinlich Fibrinoid dar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02103284
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  • 9
    Electronic Resource
    Electronic Resource
    Ultrastructural study of testis in testicular feminization (1976)
    Springer
    Archives of gynecology and obstetrics 221 (1976), S. 277-287 
    Details
    ISSN: 1432-0711
    Keywords: Electron microscopy ; Endocrinology ; Pathology ; Testicular feminization ; Testis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Testicular tissue was investigated by electron microscopy in a case of testicular feminization. The seminiferous tubules were lined by spermatogonia and by Sertoli cells. Spermatocytic maturation was not observed. Leydig cells were numerous and contained well developed, abundant vesicular smooth-surfaced endoplasmic reticulum and large mitochondria with tubulovesicular cristae. Reinke crystalloids were absent. The ultrastructural findings were consistent with the assumption that Leydig cells were under adequate stimulation and were in a stage of active secretion. Hence, the results seem to indicate that testicular endocrine function was maintained in the studied case.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00667021
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  • 10
    Electronic Resource
    Electronic Resource
    The ultrastructure of granuloma annulare (1977)
    Springer
    Archives of dermatological research 259 (1977), S. 225-234 
    Details
    ISSN: 1432-069X
    Keywords: Granuloma annulare ; Necrobiosis of collagen ; Palisading granuloma ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Neun Fälle von Granuloma annulare in verschiedenen Entwicklungsstadien wurden elektronenmikroskopisch untersucht, insbesondere Kollagenveränderungen, das entzündliche Infiltrat und Gefäßalterationen. Die nekrobiotischen Veränderungen des Kollagens bestehen in Auflockerung der Bündel vom Rande her, Schwellung der Fibrillen mit Verlust ihrer Querstreifung, völliger Desintegration des Kollagens und Niederschlägen von mucinösen Substanzen, Fibrin und Glykogen. In 2 Fällen waren sogenannte “filamentöse quergestreifte Aggregationen” (= sog. “long-spacing collagen”) nachweisbar. In späteren Stadien zeigte sich Kollagenneubildung. Im Infiltrat sind vor allem Histiocyten/Makrophagen mit Übergängen zu Epitheloidzellen und Riesenzellen, daneben auch Lymphocyten und später Neutrophile identifizierbar. Gefäßveränderungen bestehen in Endothelschwellung und Basalmembranverdoppelungen. Es besteht ein perivasculäres Infiltrat, jedoch keine echte Vaskulitis. Die Ergebnisse sprechen dafür, daß die Nekrobiose des Kollagens ohne primäre Vaskulitis ein initiales Ereignis bei Granuloma annulare darstellt. Pathogenetisch wird ein Immungeschehen vom verzögerten Typ diskutiert.
    Notes: Summary Nine cases of granuloma annulare at different stages of development were investigated electron microscopically. Special attention was given to collagen changes, to the cellular infiltrate, and to vascular changes. The necrobiotic changes of the collagen were: loosening of the fibers at the borders of the bundles; swelling and loss of cross-striation of the fibrils; and complete degradation of the collagen, with precipitation of mucinous and fibrin material and of glycogen. In 2 cases “cross-banded filamentous aggregations” were present. In later stages new collagen synthesis occurred. In the infiltrate, histiocytes/macrophages were seen developing into epithelioid and giant cells. In addition, numerous active lymphocytes and, at later stages, some neutrophils were found. Vascular changes consisted of endothelial swelling and duplications of the basal lamina. A perivascular infiltrate was present, but no distinct vasculitis was seen. Our results indicate that the necrobiosis of the collagen without primary vasculitis may be an initial event in granuloma annulare. A delayed hypersensitivity mechanism is discussed with regard to pathogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00561450
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