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  • Electronic books.  (165)
  • Oxford :Oxford University Press, Incorporated,  (165)
  • 2000-2004  (165)
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  • 1
    Online Resource
    Online Resource
    Oxford :Oxford University Press, Incorporated,
    Keywords: Population genetics.;Variation (Biology). ; Electronic books.
    Description / Table of Contents: Featuring a superb selection of papers from leading authors, this book summarizes the state of current understanding about the extent of genetic variation within wild populations and the ways to monitor such variation. It is a valuable resource for professionals and graduate students in genetics, biology, ecology, and evolution.
    Type of Medium: Online Resource
    Pages: 1 online resource (276 pages)
    Edition: 1st ed.
    ISBN: 9781602566576
    DDC: 576.5/4
    Language: English
    Note: Intro -- Contents -- Contributors -- 1. Quantitative Genetic Variation in Populations of Darwin's Finches -- 2. Adaptation, Natural Selection, and Optimal Life-History Allocation in the Face of Genetically Based Trade-offs -- 3. Natural Selection and the Evolution of Adaptive Genetic Variation in Northern Freshwater Fishes -- 4. Understanding Natural Selection on Traits That Are Influenced by Environmental Conditions -- 5. Adaptive Evolution and Neutral Variation in a Wild Leafminer Metapopulation -- 6. Reaching New Adaptive Peaks: Evolution of Alternative Bill Forms in an African Finch -- 7. Geographic Variation in Flower Size in Wild Radish: The Potential Role of Pollinators in Population Differentiation -- 8. Detecting Inheritance with Inferred Relatedness in Nature -- 9. Laboratory and Field Heritabilities: Some Lessons from Drosophila -- 10. Intra- and Interpopulation Genetic Variation: Explaining the Past and Predicting the Future -- 11. Adaptive Genetic Variation in the Wild -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- V -- W -- Y.
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  • 2
    Keywords: Geographic information systems. ; Electronic books.
    Description / Table of Contents: This volume presents a set of coherent, cross-referenced perspectives on incorporating the spatial representation and analytical power of GIS with agent-based modelling of evolutionary and non-linear processes and phenomena. Many recent advances in software algorithms for incorporating geographic data in modeling social and ecological behaviors, and successes in applying such algorithms, had not been adequately reported in the literature. This book seeks to serve as the standard guide to this broad area.
    Type of Medium: Online Resource
    Pages: 1 online resource (342 pages)
    Edition: 1st ed.
    ISBN: 9780198032748
    Series Statement: Santa Fe Institute Studies on the Sciences of Complexity Series
    DDC: 300.113
    Language: English
    Note: Intro -- Contents -- Preface -- 1 Integrating Geographic Information Systems and Agent-Based Technologies for Modeling and Simulating Social and Ecological Phenomena -- 2 Providing a Broad Spectrum of Agents in Spatially Explicit Simulation Models: The Gensim Approach -- 3 Spatial Units as Agents: Making the Landscape an Equal Player in Agent-Based Simulations -- 4 Geographic Information Systems and Agent-Based Modeling -- 5 Management Application of an Agent-Based Model: Control of Cowbirds at the Landscape Scale -- 6 Integrating Spatial Data into an Agent-Based Modeling System: Ideas and Lessons from the Development of the Across-Trophic-Level System Simulation -- 7 Models of Individual Decision Making in Agent-Based Simulation of Common-Pool-Resource Management Institutions -- 8 An Agent-Based Approach to Environmental and Urban Systems within Geographic Information Systems -- 9 Mobile Agents with Spatial Intelligence -- 10 Simulating Wildland Recreation Use and Conflicting Spatial Interactions using Rule-Driven Intelligent Agents -- 11 An Intelligent Agent-Based Model for Simulating and Evaluating River Trip Scenerios along the Colorado River in Grand Canyon National Park -- 12 Agent-Based Simulations of Household Decision Making and Land Use Change near Altamira, Brazil -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O -- P -- R -- S -- T -- U -- V -- W -- Z.
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  • 3
    Online Resource
    Online Resource
    Oxford :Oxford University Press, Incorporated,
    Keywords: Science -- Computer simulation. ; Science -- Philosophy. ; Electronic books.
    Description / Table of Contents: Computational methods such as computer simulations, Monte Carlo methods, and agent-based modeling have become the dominant techniques in many areas of science. Extending Ourselves contains the first systematic philosophical account of these new methods, and how they require a differentapproach to scientific method. Paul Humphreys draws a parallel between the ways in which such computational methods have enhanced our abilities to mathematically model the world, and the more familiar ways in which scientific instruments have expanded our access to the empirical world. Thisexpansion forms the basis for a new kind of empiricism, better suited to the needs of science than the older anthropocentric forms of empiricism. Human abilities are no longer the ultimate standard of epistemological correctness.Humphreys also includes arguments for the primacy of properties rather than objects, the need to consider technological constraints when appraising scientific methods, and a detailed account of how the path from computational template to scientific application is constructed. This last featureallows us to hold a form of selective realism in which anti-realist arguments based on formal reconstructions of theories can be avoided. One important consequence of the rise of computational methods is that the traditional organization of the sciences is being replaced by an organization foundedon computational templates.Extending Ourselves will be of interest to philosophers of science, epistemologists, and to anyone interested in the role played by computers in modern science.
    Type of Medium: Online Resource
    Pages: 1 online resource (183 pages)
    Edition: 1st ed.
    ISBN: 9780198036005
    DDC: 501/.13
    Language: English
    Note: Intro -- Contents -- 1 Epistemic Enhancers -- 1.1 Extrapolation, Conversion, and Augmentation -- 1.2 Science Neither by the People nor for the People -- 2 Scientific Empiricism -- 2.1 Empiricism and Reliable Detection -- 2.2 The Dilution Argument -- 2.3 Accuracy, Precision, and Resolution -- 2.4 The Overlap Argument -- 2.5 Property Cluster Realism -- 2.6 Bridgman Revisited -- 2.7 Instruments as Property Detectors -- 2.8 Know Thy Instruments -- 2.9 Properties -- 2.10 Epistemic Security -- 3 Computational Science -- 3.1 The Rise of Computational Science -- 3.2 Two Principles -- 3.3 Units of Analysis -- 3.4 Computational Templates -- 3.5 ''The Same Equations Have the Same Solutions'': Reorganizing the Sciences -- 3.6 Template Construction -- 3.7 Correction Sets, Interpretation, and Justification -- 3.8 Selective Realism -- 3.9 Further Consequences -- 3.10 Templates Are Not Always Built on Laws or Theories -- 3.11 The Role of Subject-Specific Knowledge in the Construction and Evaluation of Templates -- 3.12 Syntax Matters -- 3.13 A Brief Comparison with Kuhn's Views -- 3.14 Computational Models -- 4 Computer Simulations -- 4.1 A Definition -- 4.2 Some Advantages of Computer Simulations -- 4.3 The Microscope Metaphor and Technological Advances -- 4.4 Observable, Detectable, and Tractable -- 4.5 Other Kinds of Simulation -- 4.6 Agent-Based Modeling -- 4.7 Deficiencies of Simulations -- 5 Further Issues -- 5.1 Computer Simulation as Neo-Pythagoreanism -- 5.2 Abstraction and Idealization -- 5.3 Epistemic Opacity -- 5.4 Logical Form and Computational Form -- 5.5 In Practice, Not in Principle -- 5.6 Conclusion -- References -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W.
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  • 4
    Online Resource
    Online Resource
    Oxford :Oxford University Press, Incorporated,
    Keywords: Molecular dynamics -- Computer simulation. ; Electronic books.
    Description / Table of Contents: There is a continuing growth of interest in the computer simulation of materials at the atomic scale, using academic and commercial computer programs. Such programs work with very diverse models of the inter-atomic forces. This book explains how these are constructed, their scientific basis, and the approximations that are made in deriving them.
    Type of Medium: Online Resource
    Pages: 1 online resource (303 pages)
    Edition: 1st ed.
    ISBN: 9780191545290
    Series Statement: Oxford Series on Materials Modelling Series ; v.1
    DDC: 620.110113
    Language: English
    Note: Cover -- CONTENTS -- I: THE FRAMEWORK -- 1 Essential Quantum Mechanics -- 1.1 The Time-independent Schrödinger Equation -- 1.2 Wave-mechanics of Non-interacting Fermions -- 1.3 Basis Vectors and Representations -- 1.4 Periodic Boundary Conditions -- 1.5 Local Orbitals and Spherical Harmonics -- 1.6 The Variational Principle and the Schrödinger Equation -- 1.7 The Density Matrix and the Charge Density -- 1.8 The Density of States -- 1.9 Jellium -- 1.10 The Matrix Eigenvalue Problem -- 1.11 Pseudopotentials -- 2 Essential Density Functional Theory -- 2.1 What is a Functional? -- 2.2 Functional Derivatives -- 2.3 The Thomas-Fermi Model -- 2.4 The Kohn-Sham Equations -- 3 Exploiting the Variational Principle -- 3.1 The Hellmann-Feynman Theorem -- 3.2 Perturbation Theory with the Density -- 3.3 The Second-order HKS Functional -- 3.4 The Harris-Foulkes Functional and its Generalizations -- 4 Linear response theory -- 4.1 Definition of the Response Function Xe(r, r') -- 4.2 Relationship to HKS Density Functional -- 4.3 The Non-interacting Response Function -- 4.4 The Dielectric Function -- 4.5 The Error in the Harris-Foulkes Functional -- 4.6 Linear Response and the Green Function -- 4.7 Linear Response in Jellium -- 4.8 Electron-Electron Interactions in the Jellium Response -- 4.9 The Long Wavelength Limit of Response Functions in Jellium -- 4.10 Linear Response in a Perfect Crystal -- 4.11 Non-local Potentials -- II: MODELLING ATOMS WITHIN SOLIDS -- 5 Testing an interatomic force model -- 5.1 The Cohesive Energy and Crystal Structures -- 5.2 The Structural Energy Difference Theorem -- 5.3 Elastic Constants -- 5.4 Phonons -- 5.5 Point Defects -- 6 Pairwise Potentials in Simple Metals -- 6.1 Introduction -- 6.2 The Energy in Terms of Pseudopotentials -- 6.3 Periodic Boundary Conditions -- 6.4 The Effective Pairwise Interaction. , 6.5 Example: The Ashcroft Empty-core Potential -- 6.6 Asymptotic Forms of the Pair Potential -- 6.7 The Pseudoatom Picture -- 7 Tight Binding -- 7.1 Introduction -- 7.2 Non-self-consistent Tight Binding -- 7.3 Slater-Koster Parameters -- 7.4 The Repulsive Energy -- 7.5 The Tight-Binding Bond Model -- 7.6 Hellmann-Feynman Forces -- 7.7 Self-consistent Tight-Binding -- 7.8 Moments of the Density of States -- 7.9 The Recursion Method -- 7.10 Second-moment Models -- 7.11 Fourth-moment Models -- 7.12 Bond-order Potentials -- 8 Hybrid Schemes -- 8.1 Generalized Pseudopotential Theory -- 8.2 Effective Medium Theory -- 9 Ionic models -- 9.1 Introduction -- 9.2 The Rigid Ion Model Derived -- 9.3 Beyond the Rigid Ion Model -- Bibliography -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- V -- W -- Y.
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  • 5
    Online Resource
    Online Resource
    Oxford :Oxford University Press, Incorporated,
    Keywords: Artificial intelligence. ; Thought and thinking. ; Physics -- Philosophy. ; Science -- Philosophy. ; Computers. ; Electronic books.
    Description / Table of Contents: For many decades, the proponents of `artificial intelligence' have maintained that computers will soon be able to do everything that a human can do. In his bestselling work of popular science, Sir Roger Penrose takes us on a fascinating roller-coaster ride through the basic principles of physics, cosmology, mathematics, and philosophy to show that human thinking can never be emulated by a machine.
    Type of Medium: Online Resource
    Pages: 1 online resource (755 pages)
    Edition: 1st ed.
    ISBN: 9780191506406
    DDC: 006.32
    Language: English
    Note: Cover Page -- Title Page -- Copyright Page -- Note the Reader -- Acknowledgments -- Figure Acknowledgments -- Foreword -- Preface -- Contents -- Prologue -- 1 Can A Computer Have A Mind? -- Introduction -- The Turing test -- Artificial intelligence -- An AI approach to 'pleasure' and 'pain' -- Strong AI and Searle's Chinese room -- Hardware and software -- 2 Algorithms And Turing Machines -- Background to the algorithm concept -- Turing's concept -- Binary coding of numerical data -- The Church-Turing Thesis -- Numbers other than natural numbers -- The universal Turing machine -- The insolubility of Hilbert's problem -- How to outdo an algorithm -- Church's lambda calculus -- 3 Mathematics And Reality -- The land of Tor'Bled-Nam -- Real numbers -- How many real numbers are there? -- 'Reality' of real numbers -- Complex numbers -- Construction of the Mandelbrot set -- Platonic reality of mathematical concepts? -- 4 Truth, Proof, And Insight -- Hilbert's programme for mathematics -- Formal mathematical systems -- Gödel's theorem -- Mathematical insight -- Platonism or intuitionism? -- Gödel-type theorems from Turing's result -- Recursively enumerable sets -- Is the Mandelbrot set recursive? -- Some examples of non-recursive mathematics -- Is the Mandelbrot set like non-recursive mathematics? -- Complexity theory -- Complexity and computability in physical things -- 5 The Classical World -- The status of physical theory -- Euclidean geometry -- The dynamics of Galileo and Newton -- The mechanistic world of Newtonian dynamics -- Is life in the billiard-ball world computable? -- Hamiltonian mechanics -- Phase space -- Maxwell's electromagnetic theory -- Computability and the wave equation -- The Lorentz equation of motion -- runaway particles -- The special relativity of Einstein and Poincaré -- Einstein's general relativity. , Relativistic causality and determinism -- Computability in classical physics: where do we stand? -- Mass, matter, and reality -- 6 Quantum Magic And Quantum Mystery -- Do philosophers need quantum theory? -- Problems with classical theory -- The beginnings of quantum theory -- The two-slit experiment -- Probability amplitudes -- The quantum state of a particle -- The uncertainty principle -- The evolution procedures U and R -- Particles in two places at once? -- Hilbert space -- Measurements -- Spin and the Riemann sphere of states -- Objectivity and measurability of quantum states -- Copying a quantum state -- Photon spin -- Objects with large Spin -- Many-particle systems -- The 'paradox' of Einstein, Podolsky, and Rosen -- Experiments with photons: a problem for relativity? -- Schrödinger's equation -- Dirac's equation -- Quantum field theory -- Schrödinger's cat -- Various attitudes in existing quantum theory -- Where does all this leave us? -- 7 Cosmology And The Arrow Of Time -- The flow of time -- The inexorable increase of entropy -- What is entropy? -- The second law in action -- The origin of low entropy in the universe -- Cosmology and the big bang -- The primordial fireball -- Does the big bang explain the second law? -- Black holes -- The structure of space-time singularities -- How special was the big bang? -- 8 In Search Of Quantum Gravity -- Why quantum gravity? -- What lies behind the Weyl curvature hypothesis? -- Time-asymmetry in state-vector reduction -- Hawking's box: a link with the Weyl curvature hypothesis? -- When does the state-vector reduce? -- 9 Real Brains And Model Brains -- What are brains actually like? -- Where is the seat of consciousness? -- Split-brain experiments -- Blindsight -- Information processing in the visual cortex -- How do nerve signals work? -- Computer models -- Brain plasticity. , Parallel computers and the 'oneness' of consciousness -- Is there a role for quantum mechanics in brain activity? -- Quantum computers -- Beyond quantum theory? -- 10 Where Lies The Physics Of Mind? -- What are minds for? -- What does consciousness actually do? -- Natural selection of algorithms? -- The non-algorithmic nature of mathematical insight -- Inspiration, insight, and originality -- Non-verbality of thought -- Animal consciousness? -- Contact with Plato's world -- A view of physical reality -- Determinism and strong determinism -- The anthropic principle -- Tilings and quasicrystals -- Possible relevance to brain plasticity -- The time-delays of consciousness -- The strange role of time in conscious perception -- Conclusion: a child's view -- Epilogue -- References -- Index -- Footnotes.
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  • 6
    Keywords: Astronomy. ; Dynamics. ; Motion. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (775 pages)
    Edition: 1st ed.
    ISBN: 9780195351101
    DDC: 531/.11
    Language: English
    Note: Intro -- Contents -- Introduction to Volumes 1 and 2 -- 1 Preliminaries -- 1.1 Newtons's laws and their conceptual framework -- 1.2 Invariance properties of Newtonian dynamics -- 1.3 Why it took so long to find the laws of motion -- 1.4 Why the first breakthrough occurred in astronomy -- 1.5 General comments on the absolute/relative debate -- 1.6 Was dynamics discovered or invented? -- 2 Aristotle: first airing of the absolute/relative problem -- 2.1 Brief review of the period up to Aristotle -- 2.2 Aristotle: the man and his vision -- 2.3 Pre-Aristotelian geometrism -- 2.4 Aristotle's natural motions -- 2.5 The corruptible and the quintessential -- 2.6 The concept of place and the self-contained universe -- 2.7 Time in Aristotelian physics -- 3 Hellenistic astronomy: the foundations are laid -- 3.1 Historical: the Hellenistic period -- 3.2 Purely geometrical achievements and the development of trigonometry -- 3.3 Astronomical frames of reference -- 3.4 Manifestations of the law of inertia in the heavens -- 3.5 The 'flaw' from which dynamics developed -- 3.6 Kepler's laws of planetary motions -- 3.7 The zero-eccentricity and small-eccentricity forms of Kepler's laws -- 3.8 Hipparchus's theory of the apparent solar motion -- 3.9 The epicycle-deferent theory -- 3.10 First application of the epicycle-deferent theory: alternative form of Hipparchus's theory -- 3.11 Second application of the epicycle-deferent theory: the motion of the outer planets -- 3.12 Epicycle-deferent theory for the inner planets -- 3.13 The theory of the moon -- 3.14 Ptolemy and the small-eccentricity planetary system -- 3.15 Time in Ptolemaic astronomy -- 3.16 The achievement of Ptolemy and Hellenistic astronomy -- 4 The Middle Ages: first stirrings of the scientific revolution -- 4.1 Introduction -- 4.2 Kinematics -- 4.3 Dynamics. , 4.4 Cosmology and early ideas about relativity -- 5 Copernicus: the flimsy arch -- 5.1 How Copernicus came to make his discovery -- 5.2 What Copernicus did: first approximation -- 5.3 Kinematic relativity in De Revolutionibus -- 5.4 Preliminary evaluation of the significance of Copernicus's discovery -- 5.5 What Copernicus did: second approximation -- 5.6 Copernicus's concept of place and the ultimate frame of reference -- 5.7 Copernicus's concept of motion -- 5.8 The significance of the Copernican revolution: second evaluation -- 6 Kepler: the dominion of the sun -- 6.1 Brahe and Kepler -- 6.2 The dethronement of the usurper -- 6.3 The Zeroth Law, the vicarious hypothesis and the demise of the old order -- 6.4 The halving of the eccentricity of the earth's orbit -- 6.5 The First and Second Laws -- 6.6 Kepler's physics and his Third Law -- 6.7 Kepler's anticipation of Mach's Principle -- 6.8 A last look at the astronomy and evaluation of Brahe and Kepler's achievement -- 7 Galileo: the geometrization of motion -- 7.1 Brief scientific biography and general comments -- 7.2 Galileo's cosmology, overall concepts of motion, and the influence of Copernicus -- 7.3 The primordial motions: circular inertia and free fall -- 7.4 Compound motions. Parabolic motions of projectiles -- 7.5 Rotation of the earth, different forms of the law of inertia and Galilean invariance -- 7.6 Galileo and absolute motion -- 7.7 At the threshold of dynamics -- 8 Descartes and the new world -- 8.1 Introduction -- 8.2 The new world -- 8.3 The Cartesian concept of substance and the divide between materialism and idealism -- 8.4 The stone that put the stars to flight -- 8.5 The discovery of inertial motion: Descartes and Galileo compared -- 8.6 The intervention of the Inquisition -- 8.7 Descartes' early conception of motion -- 8.8 Descartes' revised concept of motion. , 9 Huygens: relativity and centrifugal force -- 9.1 Introduction -- 9.2 Collisions and relativity: general comments -- 9.3 Descartes' theory of collisions -- 9.4 Huygens' theory of collisions -- 9.5 Collisions in the centre-of-mass frame -- 9.6 The enigma of relativity -- 9.7 Centrifugal force: the work done prior to Huygens -- 9.8 Huygens' treatment of centrifugal force -- 9.9 Why Huygens failed to win the greatest prize -- 10 Newton I: the discovery of dynamics -- 10.1 Introduction -- 10.2 A comment on the significance of Newton's early work -- 10.3 Three types of force -- 10.4 Collisions -- 10.5 Centrifugal force: the paradigm of a continuously acting force -- 10.6 Newton's early applications of the formula for centrifugal force -- 10.7 The development of Newtonian dynamics -- 10.8 The Hooke-Newton correspondence of 1679 -- 10.9 The area law, Newton's treatment of time, and the solution to the Kepler problem -- 10.10 The genesis of the Principia: Ulysses draws forth Achilles -- 10.11 The Principia: its structure, fundamental concepts and most important results -- 11 Newton II: absolute or relative motion? -- 11.1 General introduction: the period up to Newton -- 11.2 Newton: general comments -- 11.3 Newton's early discussion of motion and De gravitatione -- 11.4 De gravitatione: Newton's discussion of space and body -- 11.5 The Scholium on absolute space, time, and motion -- 11.6 Comments on the Scholium -- 11.7 The absolute/relative problem in the remainder of the Principia -- 12 Post-Newtonian conceptual clarification of Newtonian dynamics -- 12.1 Introduction -- 12.2 Neumann and Body Alpha -- 12.3 Lange and the concept of inertial systems -- 12.4 Determination of the earth's polar motion from satellite observations -- 12.5 Back to the Scholium -- 12.6 Huygens and absolute motion -- 12.7 Mach's operational definition of dynamical mass. , 12.8 Synoptic overview of the discovery of dynamics -- Abbreviations for works quoted frequently in the References -- References -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- Y -- Z.
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  • 7
    Online Resource
    Online Resource
    Oxford :Oxford University Press, Incorporated,
    Keywords: RNA viruses-Research-Methodology. ; Electronic books.
    Description / Table of Contents: RNA Viruses: A Practical Approach provides a broad treatment of the principles and practice of RNA virus research to ensure the widest possible audience. RNA Viruses: A Practical Approach will be of interest to all laboratories involved in virus culture, including both academic and industrial research groups and public health laboratories.
    Type of Medium: Online Resource
    Pages: 1 online resource (287 pages)
    Edition: 1st ed.
    ISBN: 9780191565854
    Series Statement: Practical Approach Series ; v.226
    DDC: 579.25
    Language: English
    Note: Intro -- Contents -- List of protocols -- Abbreviations -- 1 Investigation of RNA virus genome structure -- 1 Introduction-the nature of the virus genome -- Properties of the genomes of RNA viruses -- Properties of the virions of RNA viruses -- Paradigm for analysis of RNA virus genome structure -- 2 Growth, assay, and purification of RNA viruses -- Source of virus: in vivo versus in vitro methods -- Assay of virus yield -- Harvesting and concentrating of virus -- Purification of virus -- Radiolabelling -- 3 RNA extraction -- Genome RNA extraction from virions -- Genome RNA extraction without virion purification -- 4 Fractionation of RNA, and analysis by Northern (RNA) blotting -- Fractionation of RNA -- Northern blotting and detection with labelled probes -- 5 Further analysis -- Characterization by restriction-endonuclease digest patterns -- Characterization by ribonuclease protection -- References -- 2 Mutagenesis of RNA virus genomes -- 1 Introduction -- 2 Generation of RNA virus mutants -- Selection of mutants from the RNA quasispecies -- Recombination of RNA virus genomes -- Reassortment of segmented RNA virus genomes -- Generation of defective RNA virus populations by passage in culture -- Recovery of RNA virus mutants from infectious cDNA -- 3 Determining the genotype of RNA virus mutants -- 4 Analysis of the frequency of RNA virus mutants -- References -- 3 Analysis of transcriptional control In RNA virus Infections -- 1 Introduction -- 2 Analysis of virus mRNAs in virus-infected cells -- General considerations -- Radiolabelling and extraction of virus RNAs -- Gel electrophoresis of RNAs -- 3 Analysis of virus mRNA structure -- General considerations -- Separation of virus RNA in preparative agarose gels -- Extraction of virus RNA from gel slices -- One-dimensional oligonucleotide fingerprinting -- Northern blot hybridization. , 4 The use of RNA reporter constructs for transcriptional assays -- RNA transcription in vitro -- RNA transfection of DI RNA construct and helper virus infection -- DNA transfection and vaccinia virus infection -- CAT reporter assay system -- Detection and quantitation of minute amount of virus RNAs -- References -- 4 Analysis of RNA virus-encoded proteinases -- 1 Introduction -- 2 Defining the proteinase type -- Inhibitor studies -- Sequence analysis -- 3 Delimiting the proteinase (domain) -- Deletion/truncation analysis -- Screening for proteolytic activity using translation systems in vitro -- 4 Bacterial expression -- Expression of inactive proteinase -- Affinity 'tagging' -- Insolubility of expressed proteinases -- 5 Substrate specificity -- 6 Artificial 'reporter' polyproteins -- References -- 5 Detection and analysis of host gene targets for oncogenic retroviruses -- 1 Introduction -- 2 Insertional mutagenesis -- Analysis of virus integration patterns -- Cloning of virus integration sites -- Analysis of host DNA flanking the provirus integration sites -- Location of genes at integration sites -- 3 Transduction of virus oncogenes -- Properties of transducing viruses -- Biological methods of detection -- Molecular methods of detection -- References -- 6 Analysis of RNA virus quasispecies -- 1 Virus quasispecies -- 2 Choice of analytical method -- 3 Nucleic acid extraction -- Reverse transcription of virus RNA -- The polymerase chain reaction as a tool for quasispecies analysis -- Polyacrylamide gel electrophoresis -- Methods for labelling and staining DNA -- 4 Single-stranded conformation polymorphism analysis -- Heteroduplex and quantitative heteroduplex tracking analyses -- Length polymorphism analysis (LPA) -- Point mutation assays -- 5 Sequence analysis -- Sequence manipulation and phylogenetic analyses -- References. , 7 In vitro replication of RNA viruses -- 1 Introduction -- 2 De-novo synthesis of poliovirus -- Isolation of poliovirus genomic RNA -- HeLa S10 extract preparation -- Translation initiation-factor preparation -- Coupled transcription and translation -- Poliovirus plaque assay -- 3 Expression of virus proteins with enzymatic and RNA binding activities -- 4 RNA binding assays -- In vitro transcription for labelled probe preparation -- Labelled nucleoprotein complex formation -- Northwestern analysis -- Western analysis -- 5 Membrane binding of virus proteins -- Indirect immunofluorescence -- In vitro membrane binding assay -- Acknowledgements -- References -- 8 Packaging of segmented and non-segmented RNA virus genomes -- 1 Introduction -- Assembly and packaging defined -- Genome selection -- 2 Genome packaging in non-segmented, negative-strand RNA viruses -- Investigation of genome packaging: analysis of nucleocapsid RNA polarity before and after packaging into virions -- 3 Introduction of site-specific mutations in the genome of negative-strand viruses -- 4 Influenza virus genome packaging -- References -- 9 RNA virus reverse genetics -- 1 Introduction -- 2 Model systems for the manipulation of RNA viruses -- Transient expression system -- Transfection and electroporation techniques -- Choice of expression system -- Generation of RNAs containing authentic 5' and 3' termini -- 3 Synthesis of RNA templates -- 4 Purification/synthesis of virus proteins required for replication -- 5 Rescue of infectious virus -- 6 Creation of mutant viruses -- 7 Analysis of mutant phenotypes -- Genomic studies of the virus mutants -- Phenotypic studies -- 8 Technical and ethical issues -- 9 Perspectives -- References -- 10 Development of RNA virus vectors for gene delivery -- 1 Introduction -- 2 Vectors based on murine retroviruses. , Design and choice of MoMLV retrovirus vectors -- MoMLV packaging cell lines -- MoMLV vector production -- Titration of MoMLV virus vector stocks -- Concentration of MoMLV virus vector stocks -- Detection of replication-competent MoMLV (RCR) vector contamination -- Infection of target cells in vitro with MoMLV vectors -- Direct transduction of target cells in vivo with MoMLV vectors -- 3 Retrovirus gene transfer vectors based on lentiviruses -- Lentivirus vector sources and design -- Packaging and pseudotyping constructs for lentivirus vectors -- Production and concentration of recombinant vector -- Ex vivo and in vivo transduction -- Safety considerations in the use of lentivirus vectors -- References -- A1 List of suppliers -- Index -- A -- B -- C -- D -- F -- G -- H -- I -- L -- M -- N -- O -- P -- R -- S -- U -- V -- W.
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  • 8
    Online Resource
    Online Resource
    Oxford :Oxford University Press, Incorporated,
    Keywords: Watson, James D.,-1928-. ; Molecular biologists-United States-Biography. ; Electronic books.
    Description / Table of Contents: Genes, Girls and Gamow is an autobiographical account of Jim Watson's life, following on from The Double Helix, the story of his and Francis Crick's discovery of the structure of DNA (published in 1968). Here is Watson adjusting to new-found fame, carrying out tantalizing experiments on the role of RNA in biology, and falling in love, in a tale of heartbreak, scientific excitement and ambition, laced with travelogue and '50s atmosphere.
    Type of Medium: Online Resource
    Pages: 1 online resource (304 pages)
    Edition: 1st ed.
    ISBN: 9780191590627
    DDC: 572.8092
    Language: English
    Note: Cover -- CONTENTS -- FOREWORD -- LIST OF ILLUSTRATIONS -- CAST OF CHARACTERS -- PREFACE -- ACKNOWLEDGMENTS -- PROLOGUE -- 1 Cambridge (England): April 1953 -- 2 Cambridge (England): April-May 1953 -- 3 Cold Spring Harbor: June 1953 -- 4 Cambridge (England): July-August 1953 -- 5 New Haven, Northern Indiana, and Pasadena: September 1953 -- 6 Pasadena, Northern Indiana, and the East Coast: October 1953-January 1954 -- 7 Bethesda, Oak Ridge National Laboratory, and Pasadena: January-February 1954 -- 8 Pasadena: February 1954 -- 9 Pasadena, Berkeley, Urbana, Gatlinburg, and the East Coast: March-April 1954 -- 10 Pasadena: May 1954 -- 11 Woods Hole: June 1954 -- 12 Woods Hole: July 1954 -- 13 Woods Hole: August 1954 -- 14 Woods Hole, New Hampshire, and Cambridge (Mass.): August 1954 -- 15 Northern Indiana and Pasadena: September 1954 -- 16 Pasadena: October 1954 -- 17 Pasadena and Berkeley: November-December 1954 -- 18 Northern Indiana, Cambridge (Mass.), and Washington D.C.: December 1954-January 1955 -- 19 Pasadena and Berkeley: February-March 1955 -- 20 The East Coast, Pasadena, and Woods Hole: March-June 1955 -- 21 Cambridge (England): July 1955 -- 22 The Continent: August 1955 -- 23 Cambridge (England), and Scotland: September 1955 -- 24 Cambridge (England): October 1955 -- 25 Tübingen, Munich, and Cambridge (England): November-December 1955 -- 26 English Lake District and Scotland: December 1955-January 1956 -- 27 Cambridge (England): January-February 1956 -- 28 Cambridge (England): February 1956 -- 29 Cambridge (England), Israel, and Egypt: March-April 1956 -- 30 Cambridge (England): May-June 1956 -- 31 Baltimore, Cold Spring Harbor, and Cambridge (Mass.): June-September 1956 -- EPILOGUE: October 1956-March 1968.
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  • 9
    Online Resource
    Online Resource
    Oxford :Oxford University Press, Incorporated,
    Keywords: Galton, Francis, -- 1822-1911.;Geneticists -- England -- Biography.;Eugenics -- History.;Genetics -- History. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (429 pages)
    Edition: 1st ed.
    ISBN: 9781602569102
    DDC: 576.5/092 B
    Language: English
    Note: Intro -- Contents -- Preface -- Prologue: Francis Galton in Perspective -- I. ANTECEDENTS AND BEGINNINGS -- 1 An Enviable Pedigree -- 2 Metamorphosis: From Birth to Medical School -- 3 A Poll Degree from Cambridge -- 4 Drifting -- II. GEOGRAPHY AND EXPLORATION -- 5 South Africa -- 6 Making Peace with Jonker Afrikaner -- 7 Expedition to Ovampoland -- 8 Fame and Marriage -- 9 Riding High with the Royal Geographical Society -- I. The Great Lakes of Africa -- 10 Riding High with the Royal Geographical Society -- II. Stanley Faces Off with the Geographers -- 11 Weather Maps and the Anticyclone -- III. THE TRIUMPH OF THE PEDIGREE -- 12 Hereditary Talent and Character -- 13 Gemmules, Rabbits, Germs, and Stirps -- 14 Nature and Nurture -- 15 Sweet Peas and Anthropometrics -- 16 Probing the Mind -- 17 Fingerprints -- 18 The Birth of Biometrics -- 19 Galton's Disciples -- 20 Evolution by Jumps -- 21 The Mendelians Trump the Biometricians -- 22 The Triumph of the Pedigree: Eugenics -- Epilogue: Out of Pandora's Box: The First International Congress of Eugenics -- Notes -- Bibliography -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- X -- Y -- Z.
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  • 10
    Online Resource
    Online Resource
    Oxford :Oxford University Press, Incorporated,
    Keywords: Science. ; Science -- History. ; Science -- Philosophy. ; Electronic books.
    Description / Table of Contents: How do scientific conjectures become laws? Why do some laws turn out to be wrong? Why does proof mean different things in different sciences? Experience shows that disentangling scientific knowledge from opinion is harder than one might expect. Full of illuminating examples and quotations, this book brings alive issues at the heart of all science.
    Type of Medium: Online Resource
    Pages: 1 online resource (306 pages)
    Edition: 1st ed.
    ISBN: 9780191527432
    DDC: 500
    Language: English
    Note: Intro -- Contents -- 1 Perception and Language -- 1.1 Preamble -- 1.2 Light and Vision -- Introduction -- The Perception of Colour -- Interpretation and Illusion -- Disorders of the Brain -- The World of a Bat -- What Do We See? -- 1.3 Language -- Physiological Aspects of Language -- Social Aspects of Language -- Objects, Concepts, and Existence -- Numbers as Social Constructs -- Notes and References -- 2 Theories of the Mind -- 2.1 Preamble -- 2.2 Mind-Body Dualism -- Plato -- Mathematical Platonism -- The Rotation of Triangles -- Descartes and Dualism -- Dualism in Society -- 2.3 Varieties of Consciousness -- Can Computers Be Conscious? -- Gödel and Penrose -- Discussion -- Notes and References -- 3 Arithmetic -- Introduction -- Whole Numbers -- Small Numbers -- Medium Numbers -- Large Numbers -- What Do Large Numbers Represent? -- Addition -- Multiplication -- Inaccessible and Huge Numbers -- Peano's Postulates -- Infinity -- Discussion -- Notes and References -- 4 How Hard can Problems Get? -- Introduction -- The Four Colour Problem -- Goldbach's Conjecture -- Fermat's Last Theorem -- Finite Simple Groups -- A Practically Insoluble Problem -- Algorithms -- How to Handle Hard Problems -- Notes and References -- 5 Pure Mathematics -- 5.1 Introduction -- 5.2 Origins -- Greek Mathematics -- The Invention of Algebra -- The Axiomatic Revolution -- Projective Geometry -- 5.3 The Search for Foundations -- 5.4 Against Foundations -- Empiricism in Mathematics -- From Babbage to Turing -- Finite Computing Machines -- Passage to the Infinite -- Are Humans Logical? -- 5.5 The Real Number System -- A Brief History -- What is Equality? -- Constructive Analysis -- Non-standard Analysis -- 5.6 The Computer Revolution -- Discussion -- Notes and References -- 6 Mechanics and Astronomy -- 6.1 Seventeenth Century Astronomy -- Galileo -- Kepler -- Newton. , The Law of Universal Gravitation -- 6.2 Laplace and Determinism -- Chaos in the Solar System -- Hyperion -- Molecular Chaos -- A Trip to Infinity -- The Theory of Relativity -- 6.3 Discussion -- Notes and References -- 7 Probability and Quantum Theory -- 7.1 The Theory of Probability -- Kolmogorov's Axioms -- Disaster Planning -- The Paradox of the Children -- The Letter Paradox -- The Three Door Paradox -- The National Lottery -- Probabilistic Proofs -- What is a Random Number? -- Bubbles and Foams -- Kolmogorov Complexity -- 7.2 Quantum Theory -- History of Atomic Theory -- The Key Enigma -- Quantum Probability -- Quantum Particles -- The Three Aspects of Quantum Theory -- Quantum Modelling -- Measuring Atomic Energy Levels -- The EPR Paradox -- Reflections -- Schrödinger's Cat -- Notes and References -- 8 Is Evolution a Theory? -- Introduction -- The Public Perception -- The Geological Record -- Dating Techniques -- The Mechanisms of Inheritance -- Theories of Evolution -- Some Common Objections -- Discussion -- Notes and References -- 9 Against Reductionism -- Introduction -- Biochemistry and Cell Physiology -- Prediction or Explanation -- Money -- Information and Complexity -- Subjective Consciousness -- The Chinese Room -- Zombies and Related Issues -- A Physicalist View -- Notes and References -- 10 Some Final Thoughts -- Order and Chaos -- Anthropic Principles -- From Hume to Popper -- Empiricism versus Realism -- The Sociology of Science -- Science and Technology -- Conclusions -- Notes and References -- Bibliography -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- X -- Y -- Z.
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