Description: Understanding the distribution and structure of biotopes is essential for marine conservation according to international legislation, such as the European Marine Strategy Framework Directive (MSFD). The biotope ‘Sea Pen and Burrowing Megafuna Communities’ is included in the OSPAR list of threatened and/or declining habitats. Accordingly, the MSFD prescribes a monitoring of this biotope by the member states of the EU. In the German North Sea, however, the distribution and spatial extent of this biotope as well as the structuring of its benthic species inventory is unknown. We used an extensive geo-referenced dataset on occurrence, abundance and biomass of the benthic infauna of the south-eastern North Sea to estimate the distribution of the biotope and to characterize the associated infauna assemblages. Sediment preferences of the burrowing megafauna, comprising decapod crustaceans and echiurids, were identified and the core distribution areas of the burrowing megafauna were modelled using Random Forests. Clusters of benthic infauna inside the core distribution areas were identified by fuzzy clustering. The burrowing megafauna occurred on a wide range of sediments with varying mud contents. The core distribution area of the burrowing megafauna was characterized by elevated mud content and a water depth of 25–55 m. The analysis of the benthic communities and their relation to sedimentological conditions identified four infauna clusters of slightly varying species composition. The biotope type ‘Sea Pen and Burrowing Megafuna Communities’ is primarily located inside the paleo valley of the river Elbe and covers an area of 4980 km2. Dedicated monitoring will have to take into account the spatial extent and the structural variability of the biotope. Our results can provide a baseline for the evaluation of the future development of the environmental status of the biotope. The maps generated herein will facilitate the communication of information relevant for environmental management to authorities and policy makers.

Description: Offshore wind power generation requires large areas of sea to accommodate its activities, with increasing claims for exclusive access. As a result, pressure is placed on other established maritime uses, such as commercial fisheries. The latter sector has often been taking a back seat in the thrust to move energy production offshore, thus leading to disagreements and conflicts among the different stakeholder groups. In recognition of the latter, there has been a growing international interest in exploring the combination of multiple maritime activities in the same area (multi-use; MU), including the re-instatement of fishing activities within, or in close proximity to, offshore wind farms (OWFs). We summarise local stakeholder perspectives from two sub-national case studies (East coast of Scotland and Germany's North Sea EEZ) to scope the feasibility of combining multiple uses of the sea, such as offshore wind farms and commercial fisheries. We combined a desk-based review with 15 semi-structured qualitative interviews with key knowledge holders from both industries, regulators, and academia to aggregate key results. Drivers, barriers and resulting effects (positive and negative) for potential multi-use of fisheries and OWFs are listed and ranked (57 factors in total). Factors are of economic, social, policy, legal, and technical nature. To date, in both case study areas, the offshore wind industry has shown little interest in multi-use solutions, unless clear added value is demonstrated and no risks to their operations are involved. In contrast, the commercial fishing sector is proactive towards multi-use projects and acts as a driving force for MU developments. We provide a range of management recommendations, based on stakeholder input, to support progress towards robust decision making in relation to multi-use solutions, including required policy and regulatory framework improvements, good practice guidance, empirical studies, capacity building of stakeholders and improvements of the consultation process. Our findings represent a comprehensive depiction of the current state and key stakeholder aspirations for multi-use solutions combining fisheries and OWFs. We believe that the pathways towards robust decision making in relation to multi-use solutions suggested here are transferable to other international locations.

Description: The abundance of the alien, Indo-Pacific damselfish Neopomacentrus cyanomos on an oil-loading platform in the southwest Gulf of Mexico indicates that widely distributed platforms could facilitate the expansion of its geo- graphic range across the western and northern fringes of the Gulf. From there it likely will spread to other areas of the Greater Caribbean. The lionfish example demonstrates that it eventually happens, and can do so rapidly. Reduced temperature effects on the physiology of this species were examined to better predict its survivability in the northern Gulf during winter, when sea surface temperatures fall as low as 15 °C along the coast. Overall, our results show that when the degree of experimental temperature decline was large and rapid, no compensation occurred and the stress response observed mostly reflected cellular processes that minimized damage. Integrated biomarker response values were significantly different between fish rapidly exposed to colder vs. warmer temperatures (declines of −4 °C each day, from 26 to 14 °C), reflected in higher values of blood metabolites and routine metabolic rates observed in fish exposed to 14 and 18 °C respectively, and lower activity of all enzymes, lower protein carbonylation, and higher oxidative damage to lipids in fish exposed to 14 °C. While the phy- siological proxies responded to minimize damage during the rapid-decrease experiment, the same proxies re- flected the consequences of compensation when fish were thermally challenged after a 45 days acclimation at 18 °C. In this case, lower values of blood metabolites and high antioxidant levels and indicators of damages underpinned its pejus lower range. Based on the results of the present work, it seems clear that low winter SSTs in the northern Gulf will slow down the colonization of the inshore area of N. cyanomos. We suggest that the use of physiological cellular stress markers on specimens acquired at the beginning of an invasion should be im- plemented in new standardized experimental protocols, including both rapid increases/decreases of temperature and post-acclimation temperature challenges, to assess the invasiveness potential of aquatic species such as this.

Description: The Antarctic krill, Euphausia superba, has evolved seasonal rhythms of physiology and behaviour to survive under the extreme photoperiodic conditions in the Southern Ocean. However, the molecular mechanisms generating these rhythms remain far from understood. The aim of this study was to investigate seasonal differences in gene expression in three different latitudinal regions (South Georgia, South Orkneys/Bransfield Strait, Lazarev Sea) and to identify genes with potential regulatory roles in the seasonal life cycle of Antarctic krill. The RNA-seq data were analysed (a) for seasonal differences between summer and winter krill sampled from each region, and (b) for regional differences within each season. A large majority of genes showed an up-regulation in summer krill in all regions with respect to winter krill. However, seasonal differences in gene expression were less pronounced in Antarctic krill from South Georgia, most likely due to the milder seasonal conditions of the lower latitudes of this region, with a less extreme light regime and food availability between summer and winter. Our results suggest that in the South Orkneys/Bransfield Strait and Lazarev Sea region, Antarctic krill entered a state of metabolic depression and regressed development (winter quiescence) in winter. Moreover, seasonal gene expression signatures seems to be driven by a photoperiodic timing system that may adapt the flexible behaviour and physiology of Antarctic krill to the highly seasonal environment according to the latitudinal region. However, at the lower latitude South Georgia region, food availability might represent the main environmental cue influencing seasonal physiology.

Description: Introduced bioengineering organisms may fundamentally change native coastal ecosystems by modifying existing benthic habitat structures and thereby habitat-specific species interactions. The introduction of the Pacific oyster Magallana gigas into the sedimentary coastal area of the south-eastern North Sea and its preferred settlement on native blue mussel shells caused a large-scale shift from monospecific Mytilus edulis beds to current mixed reefs of mussels and oysters. To investigate whether the newly developed biotic habitat affects the occurrence of associated native key organisms and their ecological functions, we studied the long-term density trajectory of the gastropod Littorina littorea and its grazing activity on barnacles attached to Pacific oyster reefs in the northern Wadden Sea. We found no significant correlation between oyster and snail densities on blue mussel beds in the last two decades, which spans a time-period from the beginning of Pacific oyster establishment to today's oyster dominance. A manipulative field experiment revealed that snail density significantly affects the recruitment success of barnacles Semibalanus balanoides on oyster shells with the highest number of barnacle recruits at snail exclusion. Thus, density and grazing activity of the snail L. littorea may control barnacle population dynamics on epibenthic bivalve beds in the Wadden Sea. This interspecific interaction was already known for blue mussel beds before the oyster invasion and, therefore, we conclude that despite the strong modifications that non-native ecosystem engineers cause in native biotic habitats, the ecological functions of associated key species can remain unchanged.

Description: Harmful algal blooms (HABs) are occurring more frequently in the world’s oceans, probably as a consequence of climate change. HABs have not been considered a serious concern in the Arctic, even though the Arctic warms faster than any other region. While phycotoxins and toxin-producing phytoplankton have been found in Arctic waters on several occasions, there is a lack of information on seasonal succession of species and whether the occurrence of harmful species correlates with the presence of their respective phycotoxins. Hence, there is no baseline to assess future changes of HABs in this area. Here, we investigated two periods, from winter to spring and from the spring bloom until summer, in Disko Bay, West Greenland and followed the succession of toxins and their producers using metabarcoding, as well as analyses of particulate and dissolved toxins. We observed a typical seasonal succession with a spring bloom dominated by diatoms, followed by dinoflagellates in summer, with the two most important potentially toxic taxa found being Pseudo-nitzschia spp. and Alexandrium ostenfeldii. The Pseudo-nitzschia spp. peak correlated with a clear increase in particulate domoic acid, reaching 0.05 pg/L. Presence of Alexandrium ostenfeldii could be linked to an increase in spirolides, up to 56.4 pg/L in the particulate phase. Generally, the majority of detected dissolved toxins followed the succession pattern of the particulate toxins with a delay in time. Our results further show that Arctic waters are a suitable habitat for various toxin producers and that the strong seasonality of this environment is reflected by changing abundances of different toxins that pose a potential threat to the ecosystem and its beneficiaries.