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Monitoring defensive responses in macroalgae – limitations and perspectives

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Abstract

As part of an ongoing research program aiming at monitoring molecular changes in the tissues and metabolite trafficking in the hydrosphere of algae subjected to chemical stresses, we are discussing the various analytical techniques that have been employed to characterize, and sometimes to quantity these metabolites. High-field multinuclear and solid-state nuclear magnetic resonance (NMR) spectroscopies are powerful tools for metabolite characterization from extracts and in vivo, but quantification and kinetic aspects show some limitations. Modern MS (mass spectrometry) is extremely useful for fingerprinting samples against databases and when dealing with very low concentrations of metabolites, the limitations being set by the type of chromatographic separation and mode of detection coupled with the mass spectrometer. Regarding chemical communication, optimization in terms of resolution and efficiency of hydrosphere chemical analysis can theoretically be achieved in a system which integrates (i) a multiparametric incubation chamber, (ii) a gasphase or a liquid-phase separation system and (iii) mass spectrometer(s) equipped with one or two detectors responding to the analytical and quantitative needs. This text reviews some of the techniques that have been employed in various types of plant metabolic studies, which may serve as a basis towards an integrative analytical strategy directly applicable to the metabolomics of selected marine macrophytes.

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Abbreviations

APCI:

Atmospheric Pressure Chemical Ionization

cITP:

Capillary Isotachophoresis (cITP)

DAD:

Diode Array Detector

ECD:

Electron Capture Detection

EI-MS:

Electron-Impact Mass Spectroscopy

ESI:

Electro-Spray Ionization

GC:

Gas Chromatography

1H NMR:

Proton Nuclear Magnetic Resonance

HPLC:

High-Performance Liquid Chromatography

HR-MAS:

High-Resolution Magic Angle Spin

HVOC:

Halogenated Volatile Organic Compound

HXO:

Hypohalous Acid

ICP-MS:

Inductively Coupled Plasma Mass Spectrometry

LC-MS:

Liquid Chromatography coupled with Mass Spectroscopy

LMWHC:

Low-MolecularWeight Halocarbon

MAA:

Mycosporin-like Amino Acids

MIP-AED:

Microwave-Induced Plasma Atomic Emission Detection

MS:

Mass Spectroscopy

MS/MS:

refers to two tandem-coupled mass spectrometers

NMR:

Nuclear Magnetic Resonance

31P NMR:

Phosphorus Nuclear Magnetic Resonance

PAR:

Photosynthetic Active Radiation

SBSE:

Stir Bar Sorptive Extraction

SIM:

Single Ion Monitoring

SPME:

Solid-Phase Micro Extraction

UV:

Ultra-Violet

UVR:

Ultra-Violet Radiation

VHC:

Volatile HaloCarbon

VHOC:

Volatile Halogenated Organic Compounds

X-:

Halide Ions

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Authors and Affiliations

  1. UMR 7139 CNRS/GOEMAR/UPMC, Végétaux Marins et Biomolécules, Station Biologique, Roscoff Cedex, B.P. 74, France

    S. L. La. Barre, F. Weinberger & P. Potin

  2. Laboratoire de Résonance Magnétique Nucléaire, Faculté des Sciences, Université de Bretagne Occidentale, B.P. 809, 29285, Brest Cedex, France

    N. Kervarec

Authors
  1. S. L. La. Barre
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  2. F. Weinberger
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  3. N. Kervarec
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  4. P. Potin
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Correspondence to S. L. La. Barre.

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Barre, S.L.L., Weinberger, F., Kervarec, N. et al. Monitoring defensive responses in macroalgae – limitations and perspectives. Phytochem Rev 3, 371–379 (2004). https://doi.org/10.1007/s11101-005-1459-3

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