Skip to main content
SpringerLink
Log in

Determination of the Surface Tension of Block Copolymer Micelles by Phagocytosis

  • Published:
Pharmaceutical Research Aims and scope Submit manuscript

Abstract

Purpose. This work was carried out to determine the surface tension of block copolymer micelles of 14C labelled ABA poly (oxyethylene-bi-isoprene-b-oxyethylene) which have a long circulating half life in animals.

Methods. The method used was that of phagocytosis. The percentage of micelles phagocytosed by human mononuclear cells was determined in solutions of different surface tension.

Results. The values obtained were 72 mN/m which may be predicted for a particle with a long circulating half life in animals. The method also gave an estimate of the surface tension for the mononuclear cells.

Conclusions. This technique has the advantage of determining the surface tension of highly hydrated small particles including stable micelles in an environment similar to that in which they normally exist.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. D.D. Lasic, F.J. Martin, A. Gabizon, S.K. Huang, and D. Papahadjopoulos. Sterically stabilized liposomes: a hypothesis on the molecular origin of the extended circulation times, Biochim. Biophys. Acta 1070:187–192 (1991).

    Google Scholar 

  2. A. Rolland, J. O'Mullane, P. Goddard, L. Brookman, and K. Petrak. New Macromolecular Carriers for Drugs. 1. Prearation and Characterisation of Poly(oxyethylene-bi-isoprene-boxyethylene) Block Copolymer Aggregates, J.Appl.Polymer Sci. 44:1195–1203 (1992).

    Google Scholar 

  3. B.E. Lehnert, and C. Tech. Quantitative evaluation of opsonin independent phagocytosis by alveolar macrophages in monolayer using polystyrene microspheres, J.Immun. Methods 78:337–344 (1985).

    Google Scholar 

  4. C.J. van Oss. Interaction forces between biological and polar entities in water: How many different primary forces are there? J.Disper. Sci.Technol. 12;201–219 (1991).

    Google Scholar 

  5. S.I. Jeon, J.H. Lee, J.D. Andrade, and P.G. De Gennes, Protein-surface interactions in the presence of polyethylene oxide, J. Colloid Interf. Sci. 142:149–156 (1991).

    Google Scholar 

  6. M.J. Bridgett, M.C. Davies, and S.P. Denyer. Control of staphylococcal adhesion to polystyrene surfaces by polymer surface modification with surfactants, Biomaterials 13:411–416 (1992).

    Google Scholar 

  7. J. H. Lee, J. Kopecek, and J.D. Andrade. Protein resistant surfaces prepared by PEO containing block copolymer surfactants, J.Biomed. Mater. Res. 23:351–389 (1989).

    Google Scholar 

  8. D. Li and A.W. Neumann. Contact angles on hydrophobic solid surfaces and their interpretation, J. Colloid Interf. Sci. 148:190–200 (1992).

    Google Scholar 

  9. C.J. van Oss, C.F. Gillman, and A.W. Neumann. Thermodynamic consideration and interpretations. In H.D. Isenberg (eds), Phagocytic Engulfment and Cell Adhesiveness, Marcel Dekker Publishers, New York, 1975, pp. 84–99.

    Google Scholar 

  10. R.P. Smith, D.R. Absolom, J.K. Spelt, and A.W. Neumann. Approaches to determine the surface tension of small particles—Equation of state considerations, J.Colloid Interf. Sci. 110:521–532 (1986).

    Google Scholar 

  11. D.R. Absolom. Basic methods for the study of phagocytosis. In G. Di Sabato and J. Everse (eds), Methods Enzymology Vol 132, Academic Press, 1986, pp 95–224

  12. D.A. Bass, J.W. Parce, I.R. Dechatelet, P. Szejda, M.C. Seeds, and M. Thomas. Flow cytometric studies of oxidative product formation by neutrophils-A graded response to membrane stimulation, J. Immunol. 130:1910–1917 (1983).

    Google Scholar 

  13. C.A. Holden, C.J. Olliff, A.W. Lloyd, F. Paul, and P. Taylor. Analysis of the surface tension and zeta potential of modified small particles in relation to the extent of phagocytosis, J. Pharm. Pharmacol. 44:57P (1992).

    Google Scholar 

  14. E.I. Vargha-Butler, M. Foldvari, and M. Mezei. Study of the sedimentation behaviour of liposomal drug delivery system, Colloid Surface 42:375–389 (1989).

    Google Scholar 

  15. L. Illum, and S.S. Davis. The organ distribution and circulation time of intravenously injected colloidal carriers sterically stabilized with a block copolymer-poloxamine 908, Life Sci. 40:367–374 (1987).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre International Recherches Dermatologique, Cedex, France

    Alain Rolland

  2. SmithKline Beechams, Epsom, England

    Frank Paul

  3. Department of Pharmacy, University of Brighton, Brighton, BN2 4GJ, England

    Katherine M. Stott & Cedric J. Olliff

Authors
  1. Alain Rolland
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Frank Paul
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Katherine M. Stott
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cedric J. Olliff
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rolland, A., Paul, F., Stott, K.M. et al. Determination of the Surface Tension of Block Copolymer Micelles by Phagocytosis. Pharm Res 12, 1435–1438 (1995). https://doi.org/10.1023/A:1016214900055

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1016214900055

Search

Navigation