Overexpression of IRM1 Enhances Resistance to Aphids in Arabidopsis thaliana
Figure 1
Characteristics of mutant 3646.
(A) Phenotype of six week old Wassilewskija (WS) and activation tag mutant 3646; (B) Location of the T-DNA insert (inverted triangle) containing the transposon (grey square) between At5g65040 (IRM1) and At5g65050. Black triangles at the end of the genes indicate the gene orientation. The distance from a gene to the T-DNA is indicated below the horizontal line. Short lines above the genes represent the position of primers used for quantitative RT-PCR analysis. Diagram is not drawn to scale; (C) Quantitative RT-PCR analysis of the two genes flanking the T-DNA. Values are the means ± standard deviation of three biological replicates. The star indicates a significant difference between bars within a pair (Independent-samples t-test, P<0.05).