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Analysis of sera from breast cancer patients by radioimmunoassays

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Summary

The antibody reactivity of human breast cancer sera was evaluated by means of radioimmunoassays and established breast cancer cell lines. When tested against the MDA-MB 231 cell line, 30 of 324 sera had detectable antibody reactivity. All the positive sera, however, reacted with other cell lines as well, generally including cultures initiated from sites other than breast cancers, and often including animal cell cultures. In competition radioimmunoassays the positive sera fell into various groups, indicating that a diversity of antigens was being detected. Two patients' sera identified antigens that were expressed on breast cancer cells but that were not expressed on an assortment of other cell types. Sera like these two, which identify potentially important tumor markers, could serve as valuable reagents for the analysis of the tumor-assiciated antigens of human breast cancer cells.

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Authors and Affiliations

  1. Department of Molecular Carcinogenesis and Virology, The University of Texas System Cancer Center, MD Anderson Hospital and Tumor Institute, Texas Medical Center, 77030, Houston, Texas, USA

    James W. Davis & James M. Bowen

  2. Department of Medicine, The University of Texas System Cancer Center, MD Anderson Hospital and Tumor Institute, Texas Medical Center, 77030, Houston, Texas, USA

    Matilde Olive & Relda Cailleau

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  1. James W. Davis
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  2. Matilde Olive
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  3. Relda Cailleau
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  4. James M. Bowen
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Davis, J.W., Olive, M., Cailleau, R. et al. Analysis of sera from breast cancer patients by radioimmunoassays. Cancer Immunol Immunother 11, 225–231 (1981). https://doi.org/10.1007/BF00199495

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  • DOI: https://doi.org/10.1007/BF00199495

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