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Isensee, Kirsten; Erez, Jonathan; Stoll, Heather M (2014): Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.836733, Supplement to: Isensee, K et al. (2014): Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii(Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system. Physiologia Plantarum, 150(2), 321-338, https://doi.org/10.1111/ppl.12096

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Abstract:
Accumulation of an intracellular pool of carbon (C(i) pool) is one strategy by which marine algae overcome the low abundance of dissolved CO2 (CO2 (aq) ) in modern seawater. To identify the environmental conditions under which algae accumulate an acid-labile C(i) pool, we applied a (14) C pulse-chase method, used originally in dinoflagellates, to two new classes of algae, coccolithophorids and diatoms. This method measures the carbon accumulation inside the cells without altering the medium carbon chemistry or culture cell density. We found that the diatom Thalassiosira weissflogii [(Grunow) G. Fryxell & Hasle] and a calcifying strain of the coccolithophorid Emiliania huxleyi [(Lohmann) W. W. Hay & H. P. Mohler] develop significant acid-labile C(i) pools. C(i) pools are measureable in cells cultured in media with 2-30 µmol/l CO2 (aq), corresponding to a medium pH of 8.6-7.9. The absolute C(i) pool was greater for the larger celled diatoms. For both algal classes, the C(i) pool became a negligible contributor to photosynthesis once CO2 (aq) exceeded 30 µmol/l. Combining the (14) C pulse-chase method and (14) C disequilibrium method enabled us to assess whether E. huxleyi and T. weissflogii exhibited thresholds for foregoing accumulation of DIC or reduced the reliance on bicarbonate uptake with increasing CO2 (aq) . We showed that the C(i) pool decreases with higher CO2 :HCO3 (-) uptake rates.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Other metabolic rates; Pelagos; Phytoplankton; Single species; Thalassiosira weissflogii
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-10-15.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TableTabJacobson, Andrew R
2FigureFigJacobson, Andrew R
3SpeciesSpeciesJacobson, Andrew R
4TreatmentTreatJacobson, Andrew R
5pHpHJacobson, Andrew RPotentiometricNBS scale
6Carbon incorporation rate per cellCi inc rate/cellpmol/#/hJacobson, Andrew R
7Temperature, waterTemp°CJacobson, Andrew R
8SalinitySalJacobson, Andrew R
9Alkalinity, totalATµmol/kgJacobson, Andrew RPotentiometric titration
10Carbon, inorganic, dissolvedDICµmol/kgJacobson, Andrew RCalculated using CO2SYSexternal
11Carbon dioxideCO2µmol/kgJacobson, Andrew RCalculated using CO2SYS
12Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmJacobson, Andrew RCalculated using CO2SYS
13Cell densityCells#/mlJacobson, Andrew R
14Cell biovolumeCell biovolµm3Jacobson, Andrew R
15Chlorophyll a per cellChl a/cellpg/#Jacobson, Andrew R
16Carbon, intracellular pool per cellC in pool/cellpmol/#Jacobson, Andrew R
17Carbon, intracellular pool per cell, standard deviationC in in pool std dev/cell±Jacobson, Andrew R
18Carbon, inorganic, dissolvedDICmmol/lJacobson, Andrew RCalculatedinternal
19RatioRatioJacobson, Andrew Rinternal Ci/external Ci
20Time in minutesTimeminJacobson, Andrew R
21ReplicateReplJacobson, Andrew R
22Carbon-14 incorporation per cell14C inc/cellpmol/#Jacobson, Andrew R
23Time in secondsTimesJacobson, Andrew R
24Carbon-14, organic14C orgdpmJacobson, Andrew R
25Carbon-14, organic, standard deviation14C org std dev±Jacobson, Andrew R
26Carbon dioxide/Bicarbonate uptake ratioCO2/[HCO3]- uptJacobson, Andrew R
27Carbon dioxide/Bicarbonate uptake ratio, standard deviationCO2/[HCO3]- upt std dev±Jacobson, Andrew R
28Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
30Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
7443 data points

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