The 2023 MDPI Annual Report has
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20 pages, 10901 KiB  
Article
Modeling Pressure Gradient of Gas–Oil–Water Three-Phase Flow in Horizontal Pipes Downstream of Restrictions
by Denghong Zhou and Yilin Fan
Energies 2024, 17(12), 2849; https://doi.org/10.3390/en17122849 (registering DOI) - 10 Jun 2024
Abstract
Gas–oil–water three-phase slug flows in pipes commonly exist in the oil and gas industry as oil fields are becoming mature and water production is becoming inevitable. Although studies on multiphase flows in pipes have been ongoing for decades, most previous research has focused [...] Read more.
Gas–oil–water three-phase slug flows in pipes commonly exist in the oil and gas industry as oil fields are becoming mature and water production is becoming inevitable. Although studies on multiphase flows in pipes have been ongoing for decades, most previous research has focused on gas–liquid or oil–water two-phase flows, with limited studies on gas–liquid–liquid flows. This leads to limited modeling studies on gas–liquid–liquid flows. One factor contributing to the complexity of the gas–liquid–liquid flow is the mixing between the oil and water phases, which have closer fluid properties and low interfacial tension. Restrictions or piping components play a crucial role in altering phase mixing. Unfortunately, modeling studies that consider the effects of these restrictions are limited due to the scarcity of experimental research. To address this gap, we conducted experimental studies on a gas–liquid–liquid flow downstream of a restriction and developed a new mechanistic modeling approach to predict the pressure gradient. Our model focuses on the flow pattern where the oil and water phases are partially mixed. This work emphasizes the modeling approach. The model evaluation results show that the model outperforms other existing models, with an average absolute relative error of 6.71%. Additionally, the parametric study shows that the new modeling approach effectively captures the effects of restriction size, water cut, and gas and liquid flow rates on the three-phase slug flow pressure gradient in horizontal pipes. Most previous slug flow modeling work assumes either a stratified flow or fully dispersed flow between the oil and water phases. This work provides a novel perspective in modeling a three-phase slug flow in which the oil and water phases are partially mixed. In addition, this novel approach to modeling the restriction effects on the pressure gradient paves the way for future modeling for different types of piping components or restrictions. Full article
(This article belongs to the Section H1: Petroleum Engineering)
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15 pages, 6446 KiB  
Article
Study on Stamping–Bulging Process of Thin-Walled Superalloy Diaphragm for S-Shaped Bellows
by Zhubin He, Qingsong Zhao, Kun Zhang, Jian Ning, Yi Xu and Xianggang Ruan
Materials 2024, 17(12), 2829; https://doi.org/10.3390/ma17122829 (registering DOI) - 10 Jun 2024
Abstract
A combined stamping–bulging forming process was proposed to achieve high-precision forming of large-diameter, ultra-thin-walled, superalloy welded S-type corrugated diaphragms. The underlying principle is to enhance the diaphragm’s forming accuracy by increasing the plastic deformation region and reducing springback. Using the ABAQUS version 6.14 [...] Read more.
A combined stamping–bulging forming process was proposed to achieve high-precision forming of large-diameter, ultra-thin-walled, superalloy welded S-type corrugated diaphragms. The underlying principle is to enhance the diaphragm’s forming accuracy by increasing the plastic deformation region and reducing springback. Using the ABAQUS version 6.14 finite element analysis software, finite element models were constructed for the stamping, hydraulic bulging, and combined stamping–bulging forming processes of the welded S-type metal corrugated diaphragms. A comparative analysis was conducted on the forming processes of the welded S-type metal corrugated diaphragms under the three forming methods, focusing on equivalent stress, distribution of wall thickness, and forming accuracy. This analysis determined the optimal forming process and the corresponding process parameters for superalloy welded S-type metal corrugated diaphragms. The results show that under a constant drawing force, as the bulging pressure increases, the plastic deformation of the straight sections of the diaphragm becomes more pronounced, resulting in improved shape accuracy. The combined stamping–bulging forming process guarantees the highest degree of shape accuracy for the diaphragm. The optimal process parameters were identified as a 30 t force and a 5 MPa pressure, with a maximum shape error of 0.02 mm. Concerning a plate thickness of 0.3 mm, the maximum deviation rate was found to be 6.7%, which represents a 30% improvement over traditional stamping processes. The maximum wall thinning rate was found to be 3.3%, a 1% reduction compared to traditional stamping processes, confirming the process’s feasibility. Full article
(This article belongs to the Special Issue Advanced Sheet/Bulk Metal Forming)
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21 pages, 2537 KiB  
Article
The Present and Future of the Use Phase of Social Housing in Tucumán, Argentina: An LCA Perspective
by Vanesa Celina Saez, Alberto Quintana-Gallardo, Fernando Aitor Mendiguchía-Fontes, Beatriz Silvia Garzón and Ignacio Guillén-Guillamón
Sustainability 2024, 16(12), 4958; https://doi.org/10.3390/su16124958 (registering DOI) - 10 Jun 2024
Abstract
This research focuses on assessing the environmental impact of heating and cooling systems in public housing projects, built between 2000 and 2020, in Tucuman, Argentina. It considers current and projected climate change scenarios. The study compares existing conditions with improvements suggested by IRAM [...] Read more.
This research focuses on assessing the environmental impact of heating and cooling systems in public housing projects, built between 2000 and 2020, in Tucuman, Argentina. It considers current and projected climate change scenarios. The study compares existing conditions with improvements suggested by IRAM 11603 for a thermo-energetic transition. Anticipating future energy consumption changes is vital for proposing sustainable retrofitting options to enhance affordability and energy efficiency, while ensuring occupants’ thermal comfort. A public housing prototype in Tucumán serves as the case study. The methodology combines energy simulation and Life Cycle Assessment (LCA) to analyze current and future energy demands. The results show climate change’s potential impact on housing thermal behavior and the necessity for improvements. In the base case, cooling demand exceeds 11 kWh/m2.year, while heating demand decreases by approximately 4 kWh/m2.year. Rehabilitation could reduce cooling demand by 57% and heating demand by 32.5%, considering future climate scenarios. Active architectural strategies are proposed for enhancing thermal performance and reducing energy consumption and carbon dioxide emissions. This study underscores the importance of analyzing future scenarios and implementing strategies for the thermo-energetic transition of existing social housing. Full article
19 pages, 4926 KiB  
Article
Effect of Subsequent Subgrade on Seismic Response of the High-Speed Railway Track–Bridge System
by Biao Wei, Shuaijie Yuan, Lizhong Jiang, Yujie Yu, Binqi Xiao, Jun Chen, Ruimin Zhang, Zhixing Yang and Shuaijun Li
Appl. Sci. 2024, 14(12), 5037; https://doi.org/10.3390/app14125037 (registering DOI) - 10 Jun 2024
Abstract
As an important part of the boundary conditions on both sides of the high-speed railway track–bridge system, the seismic response of the subgrade structure is different from that of the bridge structure. This difference has become increasingly significant with the widespread adoption of [...] Read more.
As an important part of the boundary conditions on both sides of the high-speed railway track–bridge system, the seismic response of the subgrade structure is different from that of the bridge structure. This difference has become increasingly significant with the widespread adoption of continuous welded rail technology in bridge construction. Therefore, investigating the seismic response of the bridge system, with a specific focus on the longitudinal constraint effects of the subsequent subgrade track structure, is of paramount importance. Utilizing finite element software, two distinct bridge models are developed: one incorporating the subsequent subgrade track structure and another excluding it. Through nonlinear time history analysis under varying seismic intensities, it is demonstrated that the longitudinal constraint of the subsequent subgrade track structure mitigates the longitudinal displacements and internal forces in critical components of the high-speed railway track–bridge system. Concurrently, acknowledging the heightened complexity and cost associated with post-earthquake repairs of the bridge structure compared to subgrade structure, this study uses a risk transfer connecting beam device. This device can redirect seismic damage from bridge structure to subgrade structure, thereby potentially reducing post-seismic repair expenses for the bridge. Full article
(This article belongs to the Special Issue Bridge Structural Analysis)
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21 pages, 4372 KiB  
Article
Effect of Chemical Treatment of Cotton Stalk Fibers on the Mechanical and Thermal Properties of PLA/PP Blended Composites
by Feng Xu, Jin Shang, Abdukeyum Abdurexit, Ruxangul Jamal, Tursun Abdiryim, Zhiwei Li, Jiangan You, Jin Wei, Erman Su and Longjiang Huang
Polymers 2024, 16(12), 1641; https://doi.org/10.3390/polym16121641 (registering DOI) - 10 Jun 2024
Abstract
Different chemical treatment methods were employed to modify the surface of cotton stalk fibers, which were then utilized as fillers in composite materials. These treated fibers were incorporated into polylactic acid/polypropylene melt blends using the melt blending technique. Results indicated that increasing the [...] Read more.
Different chemical treatment methods were employed to modify the surface of cotton stalk fibers, which were then utilized as fillers in composite materials. These treated fibers were incorporated into polylactic acid/polypropylene melt blends using the melt blending technique. Results indicated that increasing the surface roughness of cotton stalk fibers could enhance the overall mechanical properties of the composite materials, albeit potentially leading to poor fiber–matrix compatibility. Conversely, a smooth fiber surface was found to improve compatibility with polylactic acid, while Si-O-C silane coating increased fiber regularity and interfacial interaction with the matrix, thereby enhancing heat resistance. The mechanical properties and thermal stability of the composite materials made from alkali/silane-treated fibers exhibited the most significant improvement. Furthermore, better dispersion of fibers in the matrix and more regular fiber orientation were conducive to increasing the overall crystallinity of the composite materials. However, such fiber distribution was not favorable for enhancing impact resistance, although this drawback could be mitigated by increasing the surface roughness of the reinforcing fibers. Full article
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Technical Note
Modal Analysis of Cerebrovascular Effects for Digital Health Integration of Neurostimulation Therapies—A Review of Technology Concepts
by Marcel Stefanski, Yashika Arora, Mancheung Cheung and Anirban Dutta
Brain Sci. 2024, 14(6), 591; https://doi.org/10.3390/brainsci14060591 (registering DOI) - 10 Jun 2024
Abstract
Transcranial electrical stimulation (tES) is increasingly recognized for its potential to modulate cerebral blood flow (CBF) and evoke cerebrovascular reactivity (CVR), which are crucial in conditions like mild cognitive impairment (MCI) and dementia. This study explores the impact of tES on the neurovascular [...] Read more.
Transcranial electrical stimulation (tES) is increasingly recognized for its potential to modulate cerebral blood flow (CBF) and evoke cerebrovascular reactivity (CVR), which are crucial in conditions like mild cognitive impairment (MCI) and dementia. This study explores the impact of tES on the neurovascular unit (NVU), employing a physiological modeling approach to simulate the vascular response to electric fields generated by tES. Utilizing the FitzHugh–Nagumo model for neuroelectrical activity, we demonstrate how tES can initiate vascular responses such as vasoconstriction followed by delayed vasodilation in cerebral arterioles, potentially modulated by a combination of local metabolic demands and autonomic regulation (pivotal locus coeruleus). Here, four distinct pathways within the NVU were modeled to reflect the complex interplay between synaptic activity, astrocytic influences, perivascular potassium dynamics, and smooth muscle cell responses. Modal analysis revealed characteristic dynamics of these pathways, suggesting that oscillatory tES may finely tune the vascular tone by modulating the stiffness and elasticity of blood vessel walls, possibly by also impacting endothelial glycocalyx function. The findings underscore the therapeutic potential vis-à-vis blood-brain barrier safety of tES in modulating neurovascular coupling and cognitive function needing the precise modulation of NVU dynamics. This technology review supports the human-in-the-loop integration of tES leveraging digital health technologies for the personalized management of cerebral blood flow, offering new avenues for treating vascular cognitive disorders. Future studies should aim to optimize tES parameters using computational modeling and validate these models in clinical settings, enhancing the understanding of tES in neurovascular health. Full article
18 pages, 606 KiB  
Article
Antiparasitic Evaluation of Aquiluscidin, a Cathelicidin Obtained from Crotalus aquilus, and the Vcn-23 Derivative Peptide against Babesia bovis, B. bigemina and B. ovata
by Edwin Esaú Hernández-Arvizu, Masahito Asada, Shin-Ichiro Kawazu, Carlos Agustín Vega, Angelina Rodríguez-Torres, Rodrigo Morales-García, Aldo J. Pavón-Rocha, Gloria León-Ávila, Bruno Rivas-Santiago and Juan Mosqueda
Pathogens 2024, 13(6), 496; https://doi.org/10.3390/pathogens13060496 (registering DOI) - 10 Jun 2024
Abstract
Babesiosis is a growing concern due to the increased prevalence of this infectious disease caused by Babesia protozoan parasites, affecting various animals and humans. With rising worries over medication side effects and emerging drug resistance, there is a notable shift towards researching babesiacidal [...] Read more.
Babesiosis is a growing concern due to the increased prevalence of this infectious disease caused by Babesia protozoan parasites, affecting various animals and humans. With rising worries over medication side effects and emerging drug resistance, there is a notable shift towards researching babesiacidal agents. Antimicrobial peptides, specifically cathelicidins known for their broad-spectrum activity and immunomodulatory functions, have emerged as potential candidates. Aquiluscidin, a cathelicidin from Crotalus aquilus, and its derivative Vcn-23, have been of interest due to their previously observed antibacterial effects and non-hemolytic activity. This work aimed to characterize the effect of these peptides against three Babesia species. Results showed Aquiluscidin's significant antimicrobial effects on Babesia species, reducing the B. bigemina growth rate and exhibiting IC50 values of 14.48 and 20.70 μM against B. ovata and B. bovis, respectively. However, its efficacy was impacted by serum presence in culture, and it showed no inhibition against a B. bovis strain grown in serum-supplemented medium. Conversely, Vcn-23 did not demonstrate babesiacidal activity. In conclusion, Aquiluscidin shows antibabesia activity in vitro and its efficacy is affected by the presence of serum in the culture medium. Nevertheless, this peptide represents a candidate for further investigation of its antiparasitic properties and provides insights into potential alternatives for the treatment of babesiosis. Full article
(This article belongs to the Collection Advances in Tick Research)
14 pages, 8021 KiB  
Article
Comparative Analysis of Transcriptomes to Identify Genes during Bud Dormancy of Pyrus pyrifolia ‘Huanghua’
by Huiquan Wang, Chunying Liu, Qinghua Ye, Yunyu Shen, Shaohua Wu and Lizhong Lin
Horticulturae 2024, 10(6), 619; https://doi.org/10.3390/horticulturae10060619 (registering DOI) - 10 Jun 2024
Abstract
The study of pear dormancy mechanisms is currently a major research area in pear production and has high economic significance for agricultural production. We selected the southern sand pear cultivar Pyrus pyrifolia ‘Huanghua’ as the test material to study the pear dormancy process [...] Read more.
The study of pear dormancy mechanisms is currently a major research area in pear production and has high economic significance for agricultural production. We selected the southern sand pear cultivar Pyrus pyrifolia ‘Huanghua’ as the test material to study the pear dormancy process through microscopic observation of pear flower buds. The endodormancy period is abbreviated as D_bud. Similarly, the endodormancy release initiation period and the ecodormancy period are referred to as DB_bud and G_bud, respectively. Meanwhile, RNA sequencing was used to obtain the gene expression profile of Pyrus pyrifolia ‘Huanghua’ flower buds. The RNA sequencing results indicated that there were 224 differentially expressed genes (DEGs) in endodormancy (D) vs. endodormancy release initiation period (DB), while 975 were identified between endodormancy and ecodormancy (G). Finally, a total of 868 DEGs were found in the DB vs. G comparison. The expression levels of the genes Pbr009498.1 (LAX1-1), Pbr012348.1 (LAX1-2), Pbr021158.1 (GH35), and Pbr031621.1 (LAX2) encoding IAA were significantly higher during the DB_bud than during the D_bud. The expression level of Pbr025864.2 (IAA13) during the D_bud was significantly higher than the DB_bud and G_bud. The Pbr041942.1 (GID1B) gene also showed a significant increase during ecodormancy. Taken together, these results suggest that these genes, annotated as LAX1, GH35, LAX2, IAA13, and GID1C, are involved in endodormancy maintenance and in the transition from endodormancy to ecodormancy in Pyrus pyrifolia ‘Huanghua’. Full article
(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))
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21 pages, 2593 KiB  
Article
Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation
by Camylla Janiele Lucas Tenório, Thainá dos Santos Dantas, Lucas Silva Abreu, Magda Rhayanny Assunção Ferreira and Luiz Alberto Lira Soares
Molecules 2024, 29(12), 2761; https://doi.org/10.3390/molecules29122761 (registering DOI) - 10 Jun 2024
Abstract
The content of chemical constituents in Eugenia uniflora leaf extracts correlates positively with biological activities. The experimental objective was to carry out the phytochemical screening and purification of the major polyphenols from the leaves of E. uniflora. In addition, the anti-Candida [...] Read more.
The content of chemical constituents in Eugenia uniflora leaf extracts correlates positively with biological activities. The experimental objective was to carry out the phytochemical screening and purification of the major polyphenols from the leaves of E. uniflora. In addition, the anti-Candida activity of the hydroalcoholic extract, fraction, subfractions and polyphenols purified were evaluated. After partitioning of the extract with ethyl acetate, the fractions were chromatographed on Sephadex® LH-20 gel followed by RP-flash chromatography and monitored by TLC and RP-HPLC. The samples were characterized by mass spectrometry (LC-ESI-QTOF-MS2) and subjected to the microdilution method in 96-well plates against strains of C. albicans, C. auris, and C. glabrata. Myricitrin (93.89%; w/w; m/z 463.0876), gallic acid (99.9%; w/w; m/z 169.0142), and ellagic acid (94.2%; w/w; m/z 300.9988) were recovered. The polyphenolic fraction (62.67% (w/w) myricitrin) and the ellagic fraction (67.86% (w/w) ellagic acid) showed the best antifungal performance (MIC between 62.50 and 500 μg/mL), suggesting an association between the majority constituents and the antifungal response of E. uniflora derivatives. However, there is a clear dependence on the presence of the complex chemical mixture. In conclusion, chromatographic strategies were effectively employed to recover the major polyphenols from the leaves of the species. Full article
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13 pages, 1422 KiB  
Article
Utilizing COVID-19 as a Model for Diagnostics Using an Electrochemical Sensor
by Ava Gevaerd, Emmanuelle A. Carneiro, Jeferson L. Gogola, Diego R. P. Nicollete, Erika B. Santiago, Halanna P. Riedi, Adriano Timm, João V. Predebon, Luis F. Hartmann, Victor H. A. Ribeiro, Carlos Rochitti, Gustavo L. Marques, Maira M. O. N. Loesch, Bernardo M. M. de Almeida, Sérgio Rogal-Junior and Marcus V. M. Figueredo
Sensors 2024, 24(12), 3772; https://doi.org/10.3390/s24123772 (registering DOI) - 10 Jun 2024
Abstract
This paper reports a rapid and sensitive sensor for the detection and quantification of the COVID-19 N-protein (N-PROT) via an electrochemical mechanism. Single-frequency electrochemical impedance spectroscopy was used as a transduction method for real-time measurement of the N-PROT in an immunosensor system based [...] Read more.
This paper reports a rapid and sensitive sensor for the detection and quantification of the COVID-19 N-protein (N-PROT) via an electrochemical mechanism. Single-frequency electrochemical impedance spectroscopy was used as a transduction method for real-time measurement of the N-PROT in an immunosensor system based on gold-conjugate-modified carbon screen-printed electrodes (Cov-Ag-SPE). The system presents high selectivity attained through an optimal stimulation signal composed of a 0.0 V DC potential and 10 mV RMS−1 AC signal at 100 Hz over 300 s. The Cov-Ag-SPE showed a log response toward N-PROT detection at concentrations from 1.0 ng mL−1 to 10.0 μg mL−1, with a 0.977 correlation coefficient for the phase (θ) variation. An ML-based approach could be created using some aspects observed from the positive and negative samples; hence, it was possible to classify 252 samples, reaching 83.0, 96.2 and 91.3% sensitivity, specificity, and accuracy, respectively, with confidence intervals (CI) ranging from 73.0 to 100.0%. Because impedance spectroscopy measurements can be performed with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing, even in places with limited resources, as an alternative to the common diagnostics methods. Full article
(This article belongs to the Special Issue Advances in Electrochemical Sensors for Biomedical Applications)
15 pages, 3734 KiB  
Article
Effective Denoising Algorithms for Converting Indoor Blueprints Using a 3D Laser Scanner
by Sehyeon Yoon, Sanghyun Choi and Jhonghyun An
Electronics 2024, 13(12), 2275; https://doi.org/10.3390/electronics13122275 (registering DOI) - 10 Jun 2024
Abstract
This paper focuses on converting complex 3D maps created by LiDAR and SLAM technology into simple 2D maps to make them easier to understand. While 3D maps provide a lot of useful details for robots and computer programs, they can be difficult to [...] Read more.
This paper focuses on converting complex 3D maps created by LiDAR and SLAM technology into simple 2D maps to make them easier to understand. While 3D maps provide a lot of useful details for robots and computer programs, they can be difficult to read for humans who are used to flat maps. We developed a new system to clean up these 3D maps and convert them into intuitive and accurate 2D maps. The system uses three steps designed to correct different kinds of errors found in 3D LiDAR scan data: clustering-based denoising, height-based denoising, and Statistical Outlier Removal. In particular, height-based denoising is the method we propose in this paper, an algorithm that leaves only indoor structures such as walls. The paper proposes an algorithm that considers the entire range of the point cloud, rather than just the points near the ceiling, as is the case with existing methods, to make denoising more effective. This makes the final 2D map easy to understand and useful for building planning or emergency preparedness. Our main goal is to map the interior of buildings faster and more effectively, creating 2D drawings that reflect accurate and current information. We want to make it easier to use LiDAR and SLAM data in our daily work and increase productivity. Full article
(This article belongs to the Special Issue Computer Vision Applications for Autonomous Vehicles)
25 pages, 4203 KiB  
Article
Prediction of Short- to Long-Term Cyclic Deformation Behavior and Fatigue Life of Polymers
by Thierry Barriere, Stani Carbillet, Xavier Gabrion and Sami Holopainen
Polymers 2024, 16(12), 1640; https://doi.org/10.3390/polym16121640 (registering DOI) - 10 Jun 2024
Abstract
The prediction of mechanical behavior and fatigue life is of major importance for design and for replacing costly and time-consuming tests. The proposed approach for polymers is a combination of a fatigue model and a governing constitutive model, which is formulated using the [...] Read more.
The prediction of mechanical behavior and fatigue life is of major importance for design and for replacing costly and time-consuming tests. The proposed approach for polymers is a combination of a fatigue model and a governing constitutive model, which is formulated using the Haward–Thackray viscoplastic model (1968) and is capable of capturing large deformations. The fatigue model integrates high- and low-cycle fatigue and is based on the concept of damage evolution and a moving endurance surface in the stress space, therefore memorizing the load history without requesting vague cycle-counting approaches. The proposed approach is applicable for materials in which the fatigue development is ductile, i.e., damage during the formation of microcracks controls most of the fatigue life (up to 90%). Moreover, damage evolution shows a certain asymptote at the ultimate of the low-cycle fatigue, a second asymptote at the ultimate of the high-cycle fatigue (which is near zero), and a curvature of how rapidly the transition between the asymptotes is reached. An interesting matter is that similar to metals, many polymers satisfy these constraints. Therefore, all the model parameters for fatigue can be given in terms of the Basquin and Coffin–Manson model parameters, i.e., satisfying well-defined parameters. Full article
(This article belongs to the Special Issue Computational and Experimental Approaches in Polymeric Materials)
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23 pages, 14231 KiB  
Article
Environmentally Relevant Antibiotic Concentrations Exert Stronger Selection Pressure on River Biofilm Resistomes than AMR-Reservoir Effluents
by Gabriela Flores-Vargas, Jordyn Bergsveinson and Darren R. Korber
Antibiotics 2024, 13(6), 539; https://doi.org/10.3390/antibiotics13060539 (registering DOI) - 10 Jun 2024
Abstract
Freshwater environments are primary receiving systems of wastewater and effluents, which carry low concentrations of antibiotics and antimicrobial-resistant (AMR) bacteria and genes. Aquatic microbial communities are thus exposed to environmentally relevant concentrations of antibiotics (ERCA) that presumably influence the acquisition and spread of [...] Read more.
Freshwater environments are primary receiving systems of wastewater and effluents, which carry low concentrations of antibiotics and antimicrobial-resistant (AMR) bacteria and genes. Aquatic microbial communities are thus exposed to environmentally relevant concentrations of antibiotics (ERCA) that presumably influence the acquisition and spread of environmental AMR. Here, we analyzed ERCA exposure with and without the additional presence of municipal wastewater treatment plant effluent (W) and swine manure run-off (M) on aquatic biofilm resistomes. Microscopic analyses revealed decreased taxonomic diversity and biofilm structural integrity, while metagenomic analysis revealed an increased abundance of resistance, virulence, and mobile element-related genes at the highest ERCA exposure levels, with less notable impacts observed when solely exposed to W or M effluents. Microbial function predictions indicated increased gene abundance associated with energy and cell membrane metabolism and heavy metal resistance under ERCA conditions. In silico predictions of increased resistance mechanisms did not correlate with observed phenotypic resistance patterns when whole communities were exposed to antimicrobial susceptibility testing. This reveals important insight into the complexity of whole-community coordination of physical and genetic responses to selective pressures. Lastly, the environmental AMR risk assessment of metagenomic data revealed a higher risk score for biofilms grown at sub-MIC antibiotic conditions. Full article
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23 pages, 2938 KiB  
Article
Monitoring and Evaluation of the Corrosion Behavior in Seawater of the Low-Alloy Steels BVDH36 and LRAH36
by Adrian Mazilu, Lidia Benea and Elena Roxana Axente
Int. J. Mol. Sci. 2024, 25(12), 6405; https://doi.org/10.3390/ijms25126405 (registering DOI) - 10 Jun 2024
Abstract
The purpose of this study is to evaluate the corrosion resistance in natural seawater (Năvodari area) of two types of low-alloy carbon steels BVDH36 and LRAH36 by electrochemical methods. The electrochemical methods used were the evolution of the free potential (OCP), electrochemical impedance [...] Read more.
The purpose of this study is to evaluate the corrosion resistance in natural seawater (Năvodari area) of two types of low-alloy carbon steels BVDH36 and LRAH36 by electrochemical methods. The electrochemical methods used were the evolution of the free potential (OCP), electrochemical impedance spectroscopy (EIS), polarization resistance (Rp) and corrosion rate (Vcorr), potentiodynamic polarization (PD), and cyclic voltammetry (CV). The studies were completed by ex situ characterization analyzes of the studied surfaces before and after corrosion such as: optical microscopy, scanning electron microscopy and X-ray diffraction analysis. The results of the study show us that the polarization resistance of the low-alloy carbon steel BVDH36 is higher compared to the polarization resistance of the low-alloy carbon steel LRAH36. It is also observed that with the increase in the immersion time of the samples in natural seawater, the polarization resistance of the BVDH36 alloy increases over time and finally decreases, and for the carbon steel LRAH36 the polarization resistance increases. Full article
(This article belongs to the Section Physical Chemistry and Chemical Physics)
20 pages, 24494 KiB  
Article
Formation of a Sacred Urban Landscape: Study on the Spatial Distribution of Pagodas in Mrauk-U, Myanmar
by Yan Zhou, Hong Jiang, Tianyang Lu and Xinjie Shen
Religions 2024, 15(6), 719; https://doi.org/10.3390/rel15060719 (registering DOI) - 10 Jun 2024
Abstract
Studying the correlation between religions and cities is conducive to understanding the role of worship in shaping human settlements. Mrauk-U, the capital of the Arakan Kingdom in Southeast Asia during the 15th–18th centuries, was once a regional Buddhist center, and the pagodas distributed [...] Read more.
Studying the correlation between religions and cities is conducive to understanding the role of worship in shaping human settlements. Mrauk-U, the capital of the Arakan Kingdom in Southeast Asia during the 15th–18th centuries, was once a regional Buddhist center, and the pagodas distributed throughout this city demonstrate the visible influence of Buddhism. The purpose of this study is to gain a more comprehensive understanding of the significance of Buddhism in Mrauk-U by exploring the correlation between the pagodas and the urban space. Based on first-hand spatial data, historical maps, and archaeological studies, the spatial distribution characteristics of the pagodas in Mrauk-U were analyzed using the methods of spatial and literature analysis. Their relationships with the urban structure, mountains, water systems, and open space were visualized and examined using the GIS platform; then, the hidden historical mechanisms were investigated. This study concludes that Mrauk-U’s pagodas, as urban images, have shaped its sacred urban landscape system, revealing that Buddhism played an important role in shaping Mrauk-U’s physical space and social and cultural characteristics. Full article
(This article belongs to the Special Issue Space for Worship in East Asia)
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15 pages, 1401 KiB  
Article
An Aeromagnetic Compensation Strategy for Large UAVs
by Liwei Ye, Zhentao Yu, Yaxun Zhang, Cheng Chi, Pu Cheng and Jie Chen
Sensors 2024, 24(12), 3775; https://doi.org/10.3390/s24123775 (registering DOI) - 10 Jun 2024
Abstract
Aeromagnetic surveys are widely used in geological exploration, mineral resource assessment, environmental monitoring, military reconnaissance, and other areas. It is necessary to perform magnetic compensation for interference in these fields. In recent years, large unmanned aerial vehicles (UAVs) have been more suitable for [...] Read more.
Aeromagnetic surveys are widely used in geological exploration, mineral resource assessment, environmental monitoring, military reconnaissance, and other areas. It is necessary to perform magnetic compensation for interference in these fields. In recent years, large unmanned aerial vehicles (UAVs) have been more suitable for magnetic detection missions because of the greater loads they can carry. This article proposes some methods for the magnetic compensation of large multiload UAVs. Because of the interference of the large platform and instrument noise, the standard deviations (stds) of the compensation data used in this paper are larger. At the beginning of this article, using the traditional T-L model, we avoid the shortcomings of the anti-magnetic interference ability of triaxial magnetic gate magnetometers. The direction cosine information is obtained by using an inertial navigation system, the global positioning system, and a triaxial magnetic gate magnetometer. Then, we increase the amplitude of the maneuvers in the compensation process; this reduces the multicollinearity problems in the compensation matrix to a certain extent, but it also results in greater magnetic field interference. Lastly, we employ the method of Lasso regularization Newton iteration (LRNM). Compared to the traditional methods of least squares (LS) and singular value decomposition (SVD), LRNM provides improvements of 34% and 27%, respectively. In summary, this series of schemes can be used to perform effective compensation for large multi-load UAVs and improve the actual use of large UAVs, making them more accurate in the measurement of aeromagnetic survey data. Full article
(This article belongs to the Section Vehicular Sensing)
16 pages, 8874 KiB  
Article
Recognition Model for Tea Grading and Counting Based on the Improved YOLOv8n
by Yuxin Xia, Zejun Wang, Zhiyong Cao, Yaping Chen, Limei Li, Lijiao Chen, Shihao Zhang, Chun Wang, Hongxu Li and Baijuan Wang
Agronomy 2024, 14(6), 1251; https://doi.org/10.3390/agronomy14061251 (registering DOI) - 10 Jun 2024
Abstract
Grading tea leaves efficiently in a natural environment is a crucial technological foundation for the automation of tea-picking robots. In this study, to solve the problems of dense distribution, limited feature-extraction ability, and false detection in the field of tea grading recognition, an [...] Read more.
Grading tea leaves efficiently in a natural environment is a crucial technological foundation for the automation of tea-picking robots. In this study, to solve the problems of dense distribution, limited feature-extraction ability, and false detection in the field of tea grading recognition, an improved YOLOv8n model for tea grading and counting recognition was proposed. Firstly, the SPD-Conv module was embedded into the backbone of the network model to enhance the deep feature-extraction ability of the target. Secondly, the Super-Token Vision Transformer was integrated to reduce the model’s attention to redundant information, thus improving its perception ability for tea. Subsequently, the loss function was improved to MPDIoU, which accelerated the convergence speed and optimized the performance. Finally, a classification-positioning counting function was added to achieve the purpose of classification counting. The experimental results showed that, compared to the original model, the precision, recall and average precision improved by 17.6%, 19.3%, and 18.7%, respectively. The average precision of single bud, one bud with one leaf, and one bud with two leaves were 88.5%, 89.5% and 89.1%. In this study, the improved model demonstrated strong robustness and proved suitable for tea grading and edge-picking equipment, laying a solid foundation for the mechanization of the tea industry. Full article
(This article belongs to the Section Precision and Digital Agriculture)
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19 pages, 1628 KiB  
Article
A Multiscale Statistical Analysis of Rough Surfaces and Applications to Tribology
by Feodor M. Borodich, Andrey Pepelyshev and Xiaoqing Jin
Mathematics 2024, 12(12), 1804; https://doi.org/10.3390/math12121804 (registering DOI) - 10 Jun 2024
Abstract
Mathematical modelling of surface roughness is of significant interest for a variety of modern applications, including, but not limited to, tribology and optics. The most popular approaches to modelling rough surfaces are reviewed and critically examined. By providing counterexamples, it is shown that [...] Read more.
Mathematical modelling of surface roughness is of significant interest for a variety of modern applications, including, but not limited to, tribology and optics. The most popular approaches to modelling rough surfaces are reviewed and critically examined. By providing counterexamples, it is shown that approaches based solely on the use of the fractal geometry or power spectral density have many drawbacks. It is recommended to avoid these approaches. It is argued that the surfaces that cannot be distinguished from the original rough surfaces can be synthesised by employing the concept of the representative elementary pattern of roughness (REPR), i.e., the smallest interval (or area) of a rough surface that statistically represents the whole surface. The REPR may be extracted from surface measurement data by the use of the “moving window” technique in combination with the Kolmogorov–Smirnov statistic. Full article
(This article belongs to the Special Issue Multiscale Mathematical Modeling)
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22 pages, 1056 KiB  
Article
Common Attractors for Generalized F-Iterated Function Systems in G-Metric Spaces
by Talat Nazir and Sergei Silvestrov
Fractal Fract. 2024, 8(6), 346; https://doi.org/10.3390/fractalfract8060346 (registering DOI) - 10 Jun 2024
Abstract
In this paper, we study the generalized F-iterated function system in G-metric space. Several results of common attractors of generalized iterated function systems obtained by using generalized F-Hutchinson operators are also established. We prove that the triplet of F-Hutchinson [...] Read more.
In this paper, we study the generalized F-iterated function system in G-metric space. Several results of common attractors of generalized iterated function systems obtained by using generalized F-Hutchinson operators are also established. We prove that the triplet of F-Hutchinson operators defined for a finite number of general contractive mappings on a complete G-metric space is itself a generalized F-contraction mapping on a space of compact sets. We also present several examples in 2-D and 3-D for our results. Full article
(This article belongs to the Section General Mathematics, Analysis)
27 pages, 11804 KiB  
Article
The Effect of an Emotionalizing Sound Design on the Driver’s Choice of Headway in a Driving Simulator
by Manuel Petersen, Barbara Deml and Albert Albers
Acoustics 2024, 6(2), 541-567; https://doi.org/10.3390/acoustics6020029 (registering DOI) - 10 Jun 2024
Abstract
This study investigates the impact of emotionalizing sound design on driving behaviour, focusing on the effect of an acoustic stimulus that varies from positive to negative/threatening based on the vehicle’s time headway (THW). Our primary goal was to explore how this sound influences [...] Read more.
This study investigates the impact of emotionalizing sound design on driving behaviour, focusing on the effect of an acoustic stimulus that varies from positive to negative/threatening based on the vehicle’s time headway (THW). Our primary goal was to explore how this sound influences driving durations within specific THW ranges and the mean THW itself. The experiment utilized a control group and a within-participant setting across simulated driving scenarios. The statistical analysis showed mixed results. While participants in the control group setup did not demonstrate significant reductions in the durations of driving in lower THW ranges, a modest but significant increase in mean THW was observed when the emotionalizing sound was active. However, within-participant comparisons showed both a significant decrease in the duration of driving at lower THWs and an increase in mean THW when the negative stimulus was active, suggesting the stimulus’ effectiveness in promoting safer driving habits. These findings highlight the potential of emotionalizing sound design to influence driver behaviour towards maintaining safer distances, although the impact appears to diminish at higher THW ranges. Future research should further investigate the characteristics of sounds that effectively modify driving behaviour, aiming for broader applications in traffic safety. Full article
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14 pages, 6366 KiB  
Article
An Advanced Hall Element Array-Based Device for High-Resolution Magnetic Field Mapping
by Tan Zhou, Jiangwei Cai and Xin Zhu
Sensors 2024, 24(12), 3773; https://doi.org/10.3390/s24123773 (registering DOI) - 10 Jun 2024
Abstract
The precise mapping of magnetic fields emitted by various objects holds critical importance in the fabrication of industrial products. To meet this requirement, this study introduces an advanced magnetic detection device boasting high spatial resolution. The device’s sensor, an array comprising 256 unpackaged [...] Read more.
The precise mapping of magnetic fields emitted by various objects holds critical importance in the fabrication of industrial products. To meet this requirement, this study introduces an advanced magnetic detection device boasting high spatial resolution. The device’s sensor, an array comprising 256 unpackaged gallium arsenide (GaAs) Hall elements arranged in a 16 × 16 matrix, spans an effective area of 19.2 mm × 19.2 mm. The design maintains a 1.2 mm separation between adjacent elements. For enhanced resolution, the probe scans the sample via a motorized rail system capable of executing specialized movement patterns. A support structure incorporated into the probe minimizes the measurement distance to below 0.5 mm, thereby amplifying the magnetic signal and mitigating errors from nonparallel probe–sample alignment. The accompanying interactive software utilizes cubic spline interpolation to transform magnetic readings into detailed two- and three-dimensional magnetic field distribution maps, signifying field strength and polarity through variations in color intensity and amplitude sign. The device’s efficacy in accurately mapping surface magnetic field distributions of magnetic and magnetized materials was corroborated through tests on three distinct samples: a neodymium–iron–boron magnet, the circular magnetic array from a smartphone, and a magnetized 430 steel plate. These tests, focused on imaging quality and magnetic field characterization, underscore the device’s proficiency in nondestructive magnetic field analysis. Full article
(This article belongs to the Special Issue Advances in Magnetic Sensors and Their Applications)
23 pages, 5245 KiB  
Article
Study of the Myosin Relay Helix Peptide by Molecular Dynamics Simulations, Pump-Probe and 2D Infrared Spectroscopy
by Holly Freedman and Jack A. Tuszynski
Int. J. Mol. Sci. 2024, 25(12), 6406; https://doi.org/10.3390/ijms25126406 (registering DOI) - 10 Jun 2024
Abstract
The Davydov model was conjectured to describe how an amide I excitation created during ATP hydrolysis in myosin might be significant in providing energy to drive myosin’s chemomechanical cycle. The free energy surfaces of the myosin relay helix peptide dissolved in 2,2,2-trifluoroethanol (TFE), [...] Read more.
The Davydov model was conjectured to describe how an amide I excitation created during ATP hydrolysis in myosin might be significant in providing energy to drive myosin’s chemomechanical cycle. The free energy surfaces of the myosin relay helix peptide dissolved in 2,2,2-trifluoroethanol (TFE), determined by metadynamics simulations, demonstrate local minima differing in free energy by only ~2 kT, corresponding to broken and stabilized hydrogen bonds, respectively. Experimental pump-probe and 2D infrared spectroscopy were performed on the peptide dissolved in TFE. The relative heights of two peaks seen in the pump-probe data and the corresponding relative volumes of diagonal peaks seen in the 2D-IR spectra at time delays between 0.5 ps and 1 ps differ noticeably from what is seen at earlier or later time delays or in the linear spectrum, indicating that a vibrational excitation may influence the conformational state of this helix. Thus, it is possible that the presence of an amide I excitation may be a direct factor in the conformational state taken on by the myosin relay helix following ATP hydrolysis in myosin. Full article
(This article belongs to the Special Issue Conformational Studies of Proteins and Peptides)
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15 pages, 947 KiB  
Article
Theoretical Insight into Psittacofulvins and Their Derivatives
by Marcin Molski
Molecules 2024, 29(12), 2760; https://doi.org/10.3390/molecules29122760 (registering DOI) - 10 Jun 2024
Abstract
Psittacofulvins are polyenal dyes responsible for coloring parrot feathers and protecting them against photo-oxidation, harmful radicals, and bacterial degradation. To explain the unusual properties of these compounds, the thermodynamic and global chemical activity descriptors characterizing four natural and three synthetic psittacofulvins, as well [...] Read more.
Psittacofulvins are polyenal dyes responsible for coloring parrot feathers and protecting them against photo-oxidation, harmful radicals, and bacterial degradation. To explain the unusual properties of these compounds, the thermodynamic and global chemical activity descriptors characterizing four natural and three synthetic psittacofulvins, as well as their hydroxyl, carboxyl and dialdehyde derivatives, were determined. To this aim, the DFT method at the B3LYP/QZVP theory level and the C-PCM solvation model were used. The calculations enabled the selection of the projected compounds for the greatest bioactivity and potential applicability as multifunctional ingredients in medicines, cosmetics, supplements, and food, in which they may play a triple role as preservative, radical scavenger, and coloring agent. The results obtained provide arguments for the identification of a fifth psittacofulvin within the parrot feather pigment, characterized by ten conjugated double bonds (docosadecaenal). Full article
(This article belongs to the Special Issue Natural Antioxidants, Dyes and Their Synthetic Analogs)

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