Expression of the galanin system in immune cells: hypothetical key players of the immune response.
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1
Paracelsus Medical University, Department of Pediatrics, Austria
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2
Paracelsus Medical University, Department of Pediatrics, Austria
Neuropeptides participate in innate immunity and tolerance. Galanin is a neuropeptide with a widespread distribution in the central and peripheral nervous systems. Several in vivo studies indicate that galanin plays a role in inflammation. The aim of the present study was to identify immune cells expressing components of the galanin system including, not only the peptide but also its three receptors (GalR1-3).
Quantitative RT-PCR analysis was performed on mRNA isolated from different types of human primary immune cells as well as human immune cell lines. We observed the expression of galanin in NK cells, skin mast cells, monocyte and macrophages. Accordingly, galanin expression was detected in the human mast cell line LDA2 and human monocyte cell line THP1.
The analysis of GalR1, GalR2 and GalR3 revealed variable expression, which did not correlate with each other. GalR1 was only expressed by skin mast cells and THP1. GalR2 was detected in polymorphonuclear cells (PMNs), monocytes, macrophages, skin mast cells, LDA2 and THP1. GalR3mRNA expression was observed in monocytes, LDA2 and THP1. Moreover, flow cytometric analyses (FACS) revealed expression of GalR3 on T cells, monocytes, B cells and a minority of PMNs.
Our results show the presence of galanin and GalRs in several types of immune cells. Experiments on different types of immune cells are under way to elucidate the functional role of galanin on immune cells expressing GalRs.
Funding: Austrian Research Promotion Agency (FFG: 822782/THERAPEP)
Keywords:
Neuropeptides,
immune cell expression,
Galanin,
galanin receptors,
RT-PCR
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Bianchini
R,
Koller
A,
Lang
A,
Wiesmayr
S,
Brodowicz
B,
Mueller
E and
Kofler
B
(2013). Expression of the galanin system in immune cells: hypothetical key players of the immune response..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00807
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Received:
24 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Rodolfo Bianchini, Paracelsus Medical University, Department of Pediatrics, Salzburg, Salzburg, A-5020, Austria, r.bianchini@salk.at