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The influence of fullerenol on the cell number, cell area and colony forming unit ability in irradiated human erythroleukemic cell line

Ičević Ivana (Prirodno-matematički fakultet, Departman za hemiju, Novi Sad)
Bogdanović Višnja (Institut za onkologiju, Zavod za eksperimentalnu onkologiju, Sremska Kamenica)
Žikić Dragan (Poljoprivredni fakultet, Departman za stočarstvo, Novi Sad)
Šolajić Slavica (Institut za onkologiju, Zavod za eksperimentalnu onkologiju, Sremska Kamenica)
Bogdanović Gordana (Institut za onkologiju, Zavod za eksperimentalnu onkologiju, Sremska Kamenica)
Đorđević Aleksandar (Prirodno-matematički fakultet, Departman za hemiju, Novi Sad)

DET (dye exclusion test) cell count and cell area by computer analysis of the images were determined in cell lines of human eritroleukemia (K562), which were irradiated with X-rays in one dose of 24 Gy and pretreated with 10 nmol/mL fullerenol (Cgo(OH)24). Cell samples obtained using a citocentrifuge and May-Grünvald Giemsi (MGG) during, were analyzed. The cell colony formation ability was monitored using quantative CFU (colony forming unit) test. Irradiation decreases the number of K562 cells, but fullerenol significantly increases cell number on 24th and 48th hour of the experiment. Cell area is larger, and the number of formed cell colonies after irradiation is significantly smaller compared to pretreated groups during the whole experiment. Pretreatment with fullerenol maintains a smaller cell area, and the number of colony formed units was larger compared to the irradiated cells.

Keywords: fullerenol, X-Ray, cell lines