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The influence of fullerenol on antioxidative enzyme activity in irradiated human erythroleukemic cell line (K562)

Bogdanović Višnja (Institut za onkologiju, Zavod za eksperimentalnu onkologiju, Sremska Kamenica)
Stankov Karmen (Medicinski fakultet, Departman za biohemiju, Novi Sad)
Nikolić Aleksandra (Institut za biološka istraživanja 'Dr Siniša Stanković', Beograd)
Ičević Ivana (Prirodno-matematički fakultet, Departman za hemiju, Novi Sad)
Šolajić Slavica (Institut za onkologiju, Zavod za eksperimentalnu onkologiju, Sremska Kamenica)
Bogdanović Gordana (Institut za onkologiju, Zavod za eksperimentalnu onkologiju, Sremska Kamenica)
Đorđević Aleksandar (Prirodno-matematički fakultet, Departman za hemiju, Novi Sad)

Cell culture K562 samples were treated with fullerenol (C6o(OH)24) at a concentration of 10 nmol/mL and thereafter irradiated with X-rays (24Gy). The activity of gamma-glutamyltransfrease (γ-GT), total superoxide-dismutase (SOD) and glutathion-peroxidase (GSH-Px) was determined 1, 24 and 48 hours after irradiation. Irradiation induces an increase in the activity of all the investigated enzymes. Fullerenol in the applied dose decreased the γ-GT activity 24 and 48 h after irradiation. The total SOD activity is increased in both pretreated groups except in the iradiated group at the 48th hour. Treatment with fullerenol before irradiation increased GSH-Px activity in irradiated groups and decreased it in the control groups.

Keywords: X-irradiation, Cell culture, Antioxidative enzymes