CELF4 Regulates Translation and Local Abundance of a Vast Set of mRNAs, Including Genes Associated with Regulation of Synaptic Function
Figure 7
Visualization of Celf4 genotype-dependent shift of CELF4 target mRNAs between polysome fractions and along the cell body/neuropil axis of CA1 hippocampal neurons.
Each chart shows the entire space examined in interaction ANOVA models described in the text and summarized on Table 2, for the hippocampal CA1 cell body vs. neuropil experiment (left panel) and the monosomes vs. polysomes experiment (right panel). Each square shows individual genes (dots), plotting the rank of their iCLIP occupancy score (X axis) against the rank of their interaction F-statistic (Y axis). Less likely CELF4 targets (below the top 2,000 occupancy scores) are de-emphasized by masking, and the most likely 2,000 CELF4 targets are unmasked on the right of each square. Density contouring (applied using a standard graphing in JMP software) reveals where CELF4 target rank is most associated with differential expression. The dashed line at the right of each divides the most likely CELF4 targets in half by F-statistic score—these two halves are compared to each other for the analysis shown in Figure 8. We note that the monosomes vs. polysomes experiment did show strong contouring in the corresponding upper-left quadrant. These entries correspond largely to mitochondrial mRNAs or nuclear or mitochondrial mRNAs that encode ribosomal proteins (data not shown).