The Over-Expression of Two Transcription Factors, ABS5/bHLH30 and ABS7/MYB101, Leads to Upwardly Curly Leaves
Figure 6
A. Co-segregation analysis of abs7-1D. 16 randomly selected plants from the F2 progeny from a cross between wild type and abs7-1D were used for analysis. Southern blot analysis was performed as in Figure 2A. Plants with abs7-1D-like phenotypes were marked by asterisks. B. Schematic representation of the T-DNA insertion site in abs7-1D. Genes in the vicinity of the T-DNA insertion site were represented by white boxes. Arrows indicated the orientation of the ORFs of these genes. C. Phenotypes of representative three-week-old wild type and two ABS7 over-expression lines (OE-8 and OE-10). D. Expression levels of ABS7 gene in wild type and two ABS7 OE lines analyzed via semi-quantitative RT-PCR. E. Semi-quantitative RT-PCR analysis of ABS7 gene expression in roots, two-week-old seedlings, rosette leaves, stems, cauline leaves, siliques and flowers. The expression of Actin2 was used as a control. F. Nuclear localization of ABS7-GFP fusion protein in Arabidopsis leaf protoplasts. Protoplast transformation, nucleus staining and fluorescence microscopy was performed as in Figure 3B.