Granulocyte/Macrophage Colony-Stimulating Factor Influences Angiogenesis by Regulating the Coordinated Expression of VEGF and the Ang/Tie System
Figure 6
Time course of changes in MMP-2 and MMP-9 expression in the wounds.
(A) MMP-2 mRNA levels in the control and GM-CSF treatment groups were assessed by qRT-PCR. (B) Immunohistochemical staining of MMP-2 protein in the two groups at day 3 after the burn event. Scale bar = 100 mm. (C) Representative Western blots showing MMP-2 protein levels in the two groups during the healing process. (D) Statistical analysis of MMP-2 protein levels. (E) qRT-PCR analysis of MMP-9 mRNA expression levels in the two groups. (F) Immunohistochemical staining for MMP-9 protein at day 3 after the burn event. Scale bar = 100 mm. (G) Representative Western blots showing MMP-9 protein levels during the healing process. (H) Statistical analysis of MMP-9 protein levels. All data above are expressed as the mean±SD (*P<0.05).