CELF4 Regulates Translation and Local Abundance of a Vast Set of mRNAs, Including Genes Associated with Regulation of Synaptic Function
Figure 6
Celf4 genotype-dependent whole cell versus subcellular expression for four CELF4 targets.
A, B. Protein abundance in wildtype and Celf4 null hippocampal tissue extracts were examined by quantitative western blotting. Representative immunoblots are shown; each protein was assessed in extracts from three mice of the same genotype. Immunoblots were visualized with chemiluminescence and signal was captured with a cooled CCD camera. B. Quantification of protein abundance was performed using ImageJ. Each sample was normalized to actin. Relative mean OD values of the Celf4 null samples compared to wildtype samples are shown with standard deviation. C–E. Sagittal sections from wildtype and Celf4 null mutant mouse brains were examined for CELF4 target protein expression using immunostaining. C. Representative images from the CA3 region of the hippocampus is shown. D. For cell body, fluorescence was quantitated in ImageJ by measuring the mean intensity for each positive cell body. Background was subtracted and average mean fluorescence for Celf4 null and wildtype were calculated. Data are presented as mean fluorescence of Celf4 null relative to wildtype ± sem. E. For dendrites, fluorescence was quantitated in ImageJ by measuring the mean intensity for each positive dendrite in the stratum radiatum or positive region in the stratum lacunosum-moleculare. Background was subtracted and average mean fluorescence for Celf4 null and wildtype were calculated. Data are presented as mean fluorescence of Celf4 null relative to wildtype ± sem. For D and E, statistical significance was determined using Student's |t| test: ***p<0.01.